FastQCFastQC Report
Mon 15 Aug 2016
SRR2920981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414168
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33050.13690016601992902No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30800.12758018497469936No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27910.1156091870988266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25770.10674484957136371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99400.032.0822331
GGTATCA74600.025.9681931
TCAACGC121150.025.767944
ATCAACG121950.025.6960753
CAACGCA122600.025.5275335
AACGCAG124100.025.1658216
TATCAAC124400.025.1389832
CATGGGG59300.022.4038164
ACGCAGA139900.022.2395277
CGCAGAG141600.021.889418
GAGTACT64200.021.68764512-13
AGTACTT70400.021.63709312-13
GTACATG122350.020.7540471
TACATGG125050.019.9590152
GTGTAGC17250.019.3506761
GCAGAGT156650.019.3310729
ACATGGG125750.019.2344993
AGAGTAC137350.018.9509710-11
GTACTTT74400.018.39443614-15
TACTTTT87050.017.66946214-15
Produced by FastQC (version 0.11.5)