FastQCFastQC Report
Mon 15 Aug 2016
SRR2920980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605921
Sequences flagged as poor quality0
Sequence length125
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85800.034.3351331
TCAACGC94350.030.7118134
CAACGCA95050.030.4221925
ATCAACG97050.030.1643493
AACGCAG98100.029.5345366
CATGGGG88350.028.2853224
TATCAAC103250.028.2381632
ACATGGG143100.027.5259783
TACATGG147700.027.313642
GTACATG152500.026.9667721
ACGCAGA111750.025.4478197
GGTATCA61950.024.9777221
CGCAGAG113800.024.8844978
GCAGAGT128900.020.3540049
ATGGGGA53650.019.851335
GTATAGC17500.018.7044721
GTATAGG10250.018.5800511
TATACTG21950.017.6191585
ATGGGGG53550.017.5551265
TAGCCCT35850.017.2608664