FastQCFastQC Report
Mon 15 Aug 2016
SRR2920980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605921
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97850.040.248441
GGTATCA65500.038.295631
TCAACGC120300.031.7866734
ATCAACG122350.031.3018953
CAACGCA122500.031.1330345
TATCAAC128300.030.2178612
AACGCAG128050.029.872856
ACGCAGA146350.026.0173247
CATGGGG93100.025.5269494
GTACATG153900.025.4352021
ACATGGG145800.025.4095233
TACATGG152500.025.2273732
CGCAGAG149650.025.1660148
GCAGAGT163900.021.9985059
AGAGTAC149850.020.846610-11
GAGTACT80350.020.5514712-13
GTACTTT82250.019.71498114-15
ATGGGGG51950.019.3553335
GTATAGC13400.019.1193051
AGTACTT89500.018.61661712-13
Produced by FastQC (version 0.11.5)