##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920980_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3605921
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.578705135248388	31.0	30.0	34.0	26.0	34.0
2	29.616578677125762	31.0	30.0	34.0	26.0	34.0
3	29.757734293125115	31.0	30.0	34.0	26.0	34.0
4	33.28832051506397	35.0	33.0	37.0	28.0	37.0
5	32.74880287172126	35.0	33.0	37.0	26.0	37.0
6	32.51021528203197	35.0	33.0	37.0	25.0	37.0
7	32.32812893016791	35.0	32.0	37.0	23.0	37.0
8	32.23636263800566	35.0	32.0	37.0	23.0	37.0
9	33.50682807526842	37.0	33.0	39.0	22.0	39.0
10-11	33.390400261126075	37.0	33.0	39.0	21.0	39.0
12-13	33.262359741103595	37.0	33.0	39.0	19.5	39.0
14-15	34.08882793050652	38.0	33.0	40.0	18.0	41.0
16-17	34.06921047355169	38.0	33.0	40.0	18.0	41.0
18-19	34.10369750196968	38.0	33.0	40.0	18.0	41.0
20-21	34.08113807817753	38.0	33.0	40.0	18.0	41.0
22-23	34.051470900222164	38.0	33.0	40.0	18.0	41.0
24-25	34.0252885462549	38.0	33.0	40.5	18.0	41.0
26-27	33.87449475459945	38.0	33.0	40.0	18.0	41.0
28-29	33.75746404316678	38.0	33.0	40.0	17.0	41.0
30-31	33.591218027239094	38.0	32.0	40.0	16.0	41.0
32-33	33.45827723347239	38.0	32.0	40.0	16.0	41.0
34-35	33.33287945021536	38.0	32.0	40.0	15.0	41.0
36-37	33.189306559960684	38.0	32.0	40.0	11.5	41.0
38-39	33.05119662909975	38.0	32.0	40.0	9.0	41.0
40-41	32.865933280290946	38.0	31.5	40.0	9.0	41.0
42-43	32.719799185839065	38.0	31.0	40.0	8.0	41.0
44-45	32.50355498636826	38.0	31.0	40.0	7.5	41.0
46-47	32.3689832639151	38.0	31.0	40.0	2.0	41.0
48-49	32.16914874175003	37.5	31.0	40.0	2.0	41.0
50-51	31.970695281455143	37.0	30.5	40.0	2.0	41.0
52-53	31.73555590929474	37.0	30.0	40.0	2.0	41.0
54-55	31.561328853294345	37.0	30.0	40.0	2.0	41.0
56-57	31.439648012255397	37.0	30.0	40.0	2.0	41.0
58-59	31.382335469911848	36.0	30.0	40.0	2.0	41.0
60-61	31.12940660652299	36.0	29.5	40.0	2.0	41.0
62-63	30.885349124398456	36.0	29.0	39.0	2.0	41.0
64-65	30.636395251032955	35.0	29.0	39.0	2.0	41.0
66-67	30.34180795974177	35.0	29.0	39.0	2.0	41.0
68-69	30.042862558553004	35.0	28.5	38.5	2.0	40.0
70-71	29.698116375816333	35.0	28.0	37.5	2.0	40.0
72-73	29.421491347148205	35.0	28.0	37.0	2.0	39.5
74-75	29.161097400636343	34.0	28.0	37.0	2.0	39.0
76-77	28.829493352738453	34.0	28.0	36.0	2.0	39.0
78-79	28.496157015087128	34.0	27.5	36.0	2.0	38.5
80-81	28.223621371627388	34.0	27.0	35.0	2.0	37.0
82-83	27.954703250570383	34.0	26.0	35.0	2.0	37.0
84-85	27.74655212912318	34.0	26.0	35.0	2.0	36.0
86-87	27.50542940347279	34.0	26.0	35.0	2.0	36.0
88-89	27.367858447259383	34.0	26.0	35.0	2.0	36.0
90-91	27.166779305481178	33.5	26.0	35.0	2.0	35.0
92-93	27.29792610542494	34.0	26.0	35.0	2.0	35.0
94-95	27.233247899773733	34.0	26.0	35.0	2.0	35.0
96-97	27.16748273187349	34.0	26.0	35.0	2.0	35.0
98-99	27.112310142124578	34.0	26.0	35.0	2.0	35.0
100-101	26.975642422560007	34.0	25.0	35.0	2.0	35.0
102-103	25.702866618542117	31.5	23.5	34.0	2.0	35.0
104-105	26.74907201239295	33.5	25.0	35.0	2.0	35.0
106-107	26.94039553279176	34.0	25.0	35.0	2.0	35.0
108-109	26.919628438892587	34.0	25.0	35.0	2.0	35.0
110-111	26.856444026366635	34.0	25.0	35.0	2.0	35.0
112-113	26.749242703875098	34.0	25.0	35.0	2.0	35.0
114-115	26.659362476327132	34.0	25.0	35.0	2.0	35.0
116-117	26.525116884146936	34.0	25.0	35.0	2.0	35.0
118-119	26.39297962989206	34.0	25.0	35.0	2.0	35.0
120-121	26.252203112602857	34.0	25.0	35.0	2.0	35.0
122-123	26.014779719245098	34.0	24.0	35.0	2.0	35.0
124-125	24.915591606138904	32.5	19.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	155347.0
3	47747.0
4	22883.0
5	17107.0
6	18162.0
7	21331.0
8	23676.0
9	25342.0
10	26959.0
11	27620.0
12	28182.0
13	29600.0
14	31034.0
15	26230.0
16	21544.0
17	19461.0
18	17931.0
19	17042.0
20	16824.0
21	17758.0
22	20294.0
23	26614.0
24	35776.0
25	46405.0
26	56504.0
27	66628.0
28	78919.0
29	91858.0
30	107877.0
31	128040.0
32	155735.0
33	191263.0
34	244510.0
35	329897.0
36	505389.0
37	683907.0
38	224155.0
39	370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.49522436495041	19.807454119276564	13.59044406857914	25.10687744719388
2	15.845872215028797	20.959051470125353	38.503409812261346	24.691666502584503
3	18.856722113448665	24.94483978856639	29.93416272401036	26.264275373974588
4	13.150407265952232	16.94526240089075	36.31821027890116	33.586120054255844
5	13.371640857118134	38.12254117730594	34.66923356846316	13.83658439711276
6	32.660873433364316	36.981116063603906	16.888049750736034	13.469960752295734
7	27.804851938606674	32.61521787242209	21.35601239581178	18.22391779315945
8	26.06396049632537	36.28401642140508	19.61992912014795	18.032093962121603
9	26.227637860735253	15.701465146088816	19.452443292474715	38.61845370070122
10-11	25.139856178046205	26.319131848070914	27.919602165914075	20.621409807968803
12-13	25.685606038542225	23.52063287863419	28.118383764081234	22.675377318742353
14-15	22.77408883477761	25.126056993822605	25.5144015798422	26.585452591557583
16-17	22.8524872908728	28.122608520875232	26.234185287693247	22.79071890055872
18-19	22.829975973045865	27.193557153036895	27.975400704703723	22.00106616921351
20-21	23.65345960805297	26.33621490113256	27.780188677021005	22.230136813793468
22-23	23.145302767900944	26.09673555201643	27.690007743204653	23.06795393687797
24-25	22.900449135015666	26.431853636129627	27.792240105271564	22.87545712358314
26-27	22.802901648108943	26.616609237925577	27.69908957704755	22.881399536917932
28-29	22.689089848753085	26.745247659802274	27.747786355671224	22.81787613577342
30-31	23.119782992458326	26.560788852039202	27.592706425123076	22.726721730379392
32-33	22.74242945815866	26.60390578905921	27.583515276726402	23.070149476055732
34-35	22.91265305805422	26.729821968458133	27.678247219150037	22.67927775433761
36-37	23.110977894467176	26.422084977023136	27.611089758535893	22.855847369973798
38-39	23.003399752248484	26.619376299214814	27.501472680408725	22.875751268127978
40-41	23.15108539546589	26.536921144842474	27.553504111134195	22.758489348557433
42-43	23.10110631541016	26.449818009020625	27.43956820382768	23.009507471741536
44-45	23.07943781089559	26.551412685150353	27.356648214045965	23.01250128990809
46-47	23.129791270031173	26.58836258465892	27.171030957508417	23.110815187801485
48-49	23.089714797968426	26.76350156340353	27.086917968023428	23.059865670604616
50-51	23.093355886421087	26.8756396278796	26.991793762405088	23.039210723294225
52-53	23.297821685005037	26.71224108141381	26.908988623317846	23.0809486102633
54-55	23.011316272235227	26.75892120775331	27.024718924081693	23.20504359592977
56-57	23.042559798681484	26.837434381677728	26.81023629877503	23.309769520865757
58-59	22.941229665699566	26.764811864637633	27.04173810816351	23.25222036149929
60-61	22.998171009565265	26.794647445967794	27.134255666090297	23.072925878376644
62-63	22.89355191930004	26.79850578448742	27.13180069006265	23.176141606149884
64-65	22.718035292926295	26.713452012190643	27.097343389546957	23.471169305336108
66-67	22.820217895676294	26.893770101224305	26.90592134593623	23.380090657163173
68-69	23.063765784576482	26.60251515747925	26.972067352149647	23.36165170579462
70-71	22.75113061416304	26.50906682927019	27.109744615191705	23.630057941375068
72-73	22.881046765602704	26.584684195063407	26.980253573884795	23.554015465449094
74-75	22.76171009038317	26.66874890432876	26.885580855544482	23.683960149743584
76-77	22.729735082364456	26.423639657871583	27.072813794312435	23.77381146545153
78-79	22.810194553360315	26.464760704790063	27.022967552155137	23.702077189694485
80-81	22.840590379495993	26.525413785193138	26.934823197656854	23.699172637654016
82-83	22.789727015480217	26.55988181767741	26.92860511568551	23.721786051156855
84-85	22.638556920474684	26.57687732009978	26.913947962084546	23.870617797340994
86-87	22.589716086831626	26.512654912072183	26.90463809313637	23.992990907959825
88-89	22.556650553779363	26.422925147894112	26.831785617687647	24.188638680638874
90-91	22.455270970780976	26.53312087032895	26.932515439539312	24.079092719350758
92-93	22.486903696923044	26.34123203029105	27.082752546792328	24.08911172599358
94-95	22.228380037030103	26.364503803560158	27.150804052507855	24.256312106901884
96-97	22.375066412541184	26.11760639979053	27.159959121356014	24.34736806631227
98-99	22.227998037763754	26.168364807290178	27.028653115105516	24.57498403984055
100-101	22.10576055315024	26.266106885072286	27.147248346888915	24.48088421488856
102-103	22.012504121045062	26.10682220372616	27.217482856660585	24.663190818568193
104-105	22.00771858054233	26.18583787763168	26.97768169079798	24.828761851028013
106-107	21.854350556272994	26.180929066872576	27.03267354962967	24.93204682722476
108-109	21.839676001964314	26.136674571166225	27.061236133552807	24.96241329331666
110-111	21.742136387358638	26.321787446373822	27.00145111229984	24.9346250539677
112-113	21.594598874876542	26.36565375231142	27.015998741911947	25.023748630900094
114-115	21.58556139275984	26.38725284759017	26.929173518624893	25.098012241025103
116-117	21.446596587164	26.258609645632134	26.945744896687025	25.34904887051684
118-119	21.361768654335208	26.22943977053832	26.96291230182354	25.445879273302936
120-121	21.239417010852872	26.35229645057759	26.99302706596658	25.415259472602962
122-123	21.165947960875116	26.314712987761062	26.931291282596558	25.588047768767265
124-125	21.050855613148027	26.344177177008017	26.87395609979041	25.731011110053547
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	83.5
2	171.5
3	288.0
4	264.0
5	212.0
6	293.0
7	539.0
8	764.0
9	784.0
10	663.5
11	558.0
12	591.0
13	677.5
14	765.0
15	884.0
16	1058.0
17	1217.5
18	1423.5
19	1758.0
20	2076.5
21	2474.5
22	3148.0
23	4132.0
24	5488.0
25	7186.5
26	9791.0
27	13540.5
28	17780.5
29	23137.5
30	29540.0
31	37813.0
32	48309.5
33	56550.0
34	63777.0
35	72399.5
36	81786.5
37	90963.0
38	99072.0
39	105667.0
40	111539.0
41	118167.5
42	125366.0
43	132146.5
44	136044.0
45	139545.5
46	144540.5
47	147243.0
48	148699.0
49	150777.0
50	151405.5
51	147035.0
52	139128.5
53	133636.5
54	128494.5
55	118116.0
56	105446.5
57	94030.5
58	84335.0
59	74586.0
60	63445.0
61	53555.0
62	43622.5
63	32024.0
64	22719.0
65	17627.5
66	13576.5
67	10151.5
68	7570.0
69	5693.0
70	4549.5
71	3457.0
72	2557.5
73	2160.0
74	1682.0
75	1251.5
76	899.0
77	550.0
78	313.5
79	183.0
80	114.0
81	74.0
82	55.5
83	36.0
84	22.0
85	15.0
86	11.5
87	8.5
88	3.5
89	2.5
90	3.5
91	4.0
92	4.5
93	6.0
94	5.0
95	4.0
96	7.0
97	8.0
98	5.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04972377375987993
2	1.1092866427190168E-4
3	0.0016916621301465006
4	0.12385185365957824
5	0.014892173178502803
6	0.002911877437137419
7	0.005185915054711404
8	0.010621419604034586
9	0.024626163468362175
10-11	0.01939865016454881
12-13	0.018857872926223287
14-15	0.02784309473224732
16-17	0.03678671828916939
18-19	0.03958766706203492
20-21	0.021228973125035185
22-23	0.022865170923045734
24-25	0.0213260357062731
26-27	0.020202882980520094
28-29	0.02006422215018022
30-31	0.024542966970158245
32-33	0.023905127150594813
34-35	0.02767670173583947
36-37	0.030491516591738974
38-39	0.030491516591738974
40-41	0.03322313494943456
42-43	0.029728882024869653
44-45	0.0280926842268591
46-47	0.038367451755043996
48-49	0.031212552909506336
50-51	0.022116402439210398
52-53	0.029049443956204256
54-55	0.03083816866758867
56-57	0.024612297385328184
58-59	0.02745484440729567
60-61	0.04086334670116178
62-63	0.04868381753233085
64-65	0.04204196375905074
66-67	0.03749388852390277
68-69	0.02821747897416499
70-71	0.03447108242249345
72-73	0.03799306751312633
74-75	0.02287903700607972
76-77	0.02010582039928218
78-79	0.029798212440039593
80-81	0.023572341157779106
82-83	0.03663419137579553
84-85	0.020577267222437762
86-87	0.03257142904683713
88-89	0.031781062313899835
90-91	0.03369458177259014
92-93	0.024848020796905977
94-95	0.020688195886709667
96-97	0.01706914821483887
98-99	0.023586207240813097
100-101	0.022865170923045734
102-103	0.026331691681542663
104-105	0.02368326982205101
106-107	0.013782886535783785
108-109	0.016375844063139487
110-111	0.02095165146435543
112-113	0.01247947473058894
114-115	0.013977011698259614
116-117	0.012326947817215076
118-119	0.007335157924979499
120-121	0.005366174134153244
122-123	0.0037161102531087066
124-125	0.06234190932080875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3605921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.93339743371168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84371679303273	36.0831118310085
2	13.845569261728013	14.104037638071498
3	5.957125127754035	9.102498650827403
4	3.12117748505285	6.358886932293981
5	1.815242053187963	4.622822246670466
6	1.1020867766920657	3.367981428221714
7	0.7539951184140719	2.68824731204837
8	0.5145862955249367	2.0967702643130406
9	0.3710427338776891	1.700862032653493
>10	1.5713328923851064	13.400894503843272
>50	0.06889407079316022	2.3684861802427077
>100	0.03287481821595611	2.956423233456702
>500	0.0017537290045898627	0.6153822342051349
>1k	6.028443368352358E-4	0.5335955121438044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	4.1598249101963134E-5
14-15	0.0	0.0	0.0	0.0	5.5464332135950846E-5
16-17	0.0	0.0	0.0	0.0	5.5464332135950846E-5
18-19	0.0	2.7732166067975423E-5	0.0	0.0	5.5464332135950846E-5
20-21	0.0	2.7732166067975423E-5	0.0	6.933041516993855E-5	8.319649820392627E-5
22-23	0.0	2.7732166067975423E-5	0.0	3.4665207584969277E-4	1.1092866427190169E-4
24-25	0.0	2.7732166067975423E-5	0.0	6.517059025974224E-4	1.1092866427190169E-4
26-27	0.0	2.7732166067975423E-5	0.0	0.0011370188087869923	1.1092866427190169E-4
28-29	0.0	2.7732166067975423E-5	0.0	0.0032307973469191367	1.1092866427190169E-4
30-31	0.0	2.7732166067975423E-5	0.0	0.011855500994059492	1.1092866427190169E-4
32-33	0.0	2.7732166067975423E-5	0.0	0.03139281198894818	1.1092866427190169E-4
34-35	0.0	2.7732166067975423E-5	0.0	0.057682905421388875	1.1092866427190169E-4
36-37	0.0	2.7732166067975423E-5	0.0	0.09853238603951667	1.1092866427190169E-4
38-39	0.0	2.7732166067975423E-5	0.0	0.1674329526354016	1.1092866427190169E-4
40-41	0.0	2.7732166067975423E-5	0.0	0.24322496249917847	1.1092866427190169E-4
42-43	0.0	2.7732166067975423E-5	0.0	0.3191833653593631	1.1092866427190169E-4
44-45	0.0	2.7732166067975423E-5	0.0	0.40785696636171453	1.1092866427190169E-4
46-47	0.0	2.7732166067975423E-5	0.0	0.5087049882679071	1.1092866427190169E-4
48-49	0.0	2.7732166067975423E-5	0.0	0.6181915799042741	1.1092866427190169E-4
50-51	0.0	2.7732166067975423E-5	0.0	0.7325590327686048	1.1092866427190169E-4
52-53	0.0	2.7732166067975423E-5	0.0	0.8441671351091717	1.386608303398771E-4
54-55	0.0	2.7732166067975423E-5	0.0	0.9717212329388247	1.386608303398771E-4
56-57	0.0	2.7732166067975423E-5	0.0	1.113585128459553	1.386608303398771E-4
58-59	0.0	2.7732166067975423E-5	0.0	1.2710067691444156	1.386608303398771E-4
60-61	0.0	2.7732166067975423E-5	0.0	1.4358744964185295	1.386608303398771E-4
62-63	0.0	2.7732166067975423E-5	0.0	1.603557038548543	1.9412516247582795E-4
64-65	0.0	2.7732166067975423E-5	0.0	1.779309641004337	1.9412516247582795E-4
66-67	0.0	2.7732166067975423E-5	0.0	1.9876614046730365	1.9412516247582795E-4
68-69	0.0	2.7732166067975423E-5	0.0	2.214884907350993	1.9412516247582795E-4
70-71	0.0	2.7732166067975423E-5	0.0	2.4604809700489834	1.9412516247582795E-4
72-73	0.0	2.7732166067975423E-5	0.0	2.7049816121872885	2.0799124550981566E-4
74-75	0.0	2.7732166067975423E-5	0.0	2.958495208297686	2.2185732854380338E-4
76-77	0.0	2.7732166067975423E-5	0.0	3.240364944212588	2.2185732854380338E-4
78-79	0.0	2.7732166067975423E-5	0.0	3.555610341990299	2.2185732854380338E-4
80-81	0.0	2.7732166067975423E-5	0.0	3.8863857527660755	2.2185732854380338E-4
82-83	0.0	2.7732166067975423E-5	0.0	4.2334122128576865	2.2185732854380338E-4
84-85	0.0	2.7732166067975423E-5	0.0	4.589451626921388	2.357234115777911E-4
86-87	0.0	2.7732166067975423E-5	0.0	4.969007917810734	2.495894946117788E-4
88-89	0.0	2.7732166067975423E-5	0.0	5.383797925689443	2.495894946117788E-4
90-91	0.0	2.7732166067975423E-5	0.0	5.837898278969506	2.495894946117788E-4
92-93	0.0	2.7732166067975423E-5	0.0	6.303673874164187	2.495894946117788E-4
94-95	0.0	2.7732166067975423E-5	0.0	6.773442901272658	2.495894946117788E-4
96-97	0.0	2.7732166067975423E-5	0.0	7.263608936524123	3.1891990978171733E-4
98-99	0.0	2.7732166067975423E-5	0.0	7.789868385913058	3.6051815888368046E-4
100-101	0.0	2.7732166067975423E-5	0.0	8.37303424007348	3.6051815888368046E-4
102-103	0.0	2.7732166067975423E-5	0.0	8.963507519992813	3.6051815888368046E-4
104-105	0.0	2.7732166067975423E-5	0.0	9.564643817765281	3.6051815888368046E-4
106-107	0.0	2.7732166067975423E-5	0.0	10.177607884365742	3.6051815888368046E-4
108-109	0.0	2.7732166067975423E-5	0.0	10.823600960753161	3.6051815888368046E-4
110-111	0.0	2.7732166067975423E-5	0.0	11.503995789147904	3.6051815888368046E-4
112-113	0.0	2.7732166067975423E-5	0.0	12.208309610776276	3.6051815888368046E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9785	0.0	40.24844	1
GGTATCA	6550	0.0	38.29563	1
TCAACGC	12030	0.0	31.786673	4
ATCAACG	12235	0.0	31.301895	3
CAACGCA	12250	0.0	31.133034	5
TATCAAC	12830	0.0	30.217861	2
AACGCAG	12805	0.0	29.87285	6
ACGCAGA	14635	0.0	26.017324	7
CATGGGG	9310	0.0	25.526949	4
GTACATG	15390	0.0	25.435202	1
ACATGGG	14580	0.0	25.409523	3
TACATGG	15250	0.0	25.227373	2
CGCAGAG	14965	0.0	25.166014	8
GCAGAGT	16390	0.0	21.998505	9
AGAGTAC	14985	0.0	20.8466	10-11
GAGTACT	8035	0.0	20.55147	12-13
GTACTTT	8225	0.0	19.714981	14-15
ATGGGGG	5195	0.0	19.355333	5
GTATAGC	1340	0.0	19.119305	1
AGTACTT	8950	0.0	18.616617	12-13
>>END_MODULE