FastQCFastQC Report
Mon 15 Aug 2016
SRR2920979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437492
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31970.13115940483086713No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29170.11967218764205176No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28120.11536448119624598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG56900.024.5736034
GTACATG117350.024.3954521
TACATGG117150.023.5173052
ACATGGG112800.023.4214523
GTATAAT15900.020.9622441
GTATCAA123550.018.9801831
TGTCGCG1151.4382758E-418.10723912-13
TAGGACG2351.6942664E-417.723254
CGTACAC4059.405994E-917.6305643
TATACTG16150.017.3145755
GGACCGT3808.740426E-817.2224756
TATCAAC134500.017.0328482
ATGGGGA33000.016.9472965
TCAACGC134200.016.8035074
CAACGCA134100.016.7706365
AACGCAG134250.016.707586
GTAGCAC8350.016.3900893
CATGGGT18550.016.358354
GTATTAG9100.016.3510461
CCTATAC7650.016.3341983
Produced by FastQC (version 0.11.5)