FastQCFastQC Report
Mon 15 Aug 2016
SRR2920979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437492
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38570.15823641677593198No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35510.14568252941958373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30730.1260722086472489No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28750.11794910506372945No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27350.11220549646932174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA1401.0572949E-425.4968077
CATGGGG54800.022.9373994
GTACATG122400.022.8823951
ACATGGG115500.022.3325983
TACATGG121300.022.2451342
GTATCAA161050.020.7210771
AGTACTT77600.020.66351512-13
ATAGCAC13350.020.5262623
ACCGTAT2659.2589835E-720.2050178
GAGTACT74000.020.06069612-13
GTACCGT4254.1836756E-1119.5959386
TCTATAC8550.019.508563
GTACTTT77900.019.28550714-15
AGAGTAC143100.018.25197210-11
CAACGCA180000.018.1463455
TCAACGC181250.018.0770234
AACGCAG183400.017.9046846
TATCAAC184400.017.8310032
ATCAACG184750.017.830933
TAAGGTG11200.017.5301345
Produced by FastQC (version 0.11.5)