FastQCFastQC Report
Mon 15 Aug 2016
SRR2920978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186809
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83400.033.966161
GTACATG138350.030.7561861
TACATGG137750.030.0213762
TCAACGC95350.028.2679584
CAACGCA95800.028.1338525
CATGGGG88600.027.9367964
ACATGGG143150.027.9328583
TATCAAC100750.027.699072
AACGCAG97550.027.6897036
ATCAACG99400.027.3569223
GTATAGG8400.025.5052031
GGTATCA61600.024.9255391
ACGCAGA110900.024.0882077
CGCAGAG110150.023.9821538
GCAGAGT122900.019.8482279
ATGGGGG53100.019.1601165
GTATATA15250.019.1219331
AGAGTAC111600.017.53980610-11
ATGGGGA49100.017.0857545
CATGGGT23850.016.9643614
Produced by FastQC (version 0.11.5)