##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920978_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3186809
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.336118669176596	31.0	28.0	33.0	16.0	34.0
2	28.343854934512862	31.0	28.0	34.0	16.0	34.0
3	28.361281143614193	31.0	28.0	34.0	16.0	34.0
4	31.721581996285312	35.0	32.0	37.0	17.0	37.0
5	31.824269669126704	35.0	32.0	37.0	18.0	37.0
6	31.769709135376484	35.0	32.0	37.0	17.0	37.0
7	31.615441653390587	35.0	32.0	37.0	17.0	37.0
8	31.605961951281046	35.0	32.0	37.0	17.0	37.0
9	32.71084994425458	37.0	32.0	39.0	16.0	39.0
10-11	32.673370917428684	37.0	32.0	39.0	16.0	39.0
12-13	32.64442001387595	37.0	32.0	39.0	15.0	39.0
14-15	33.545750623899956	38.0	32.0	40.0	14.0	41.0
16-17	33.436216917926366	38.0	32.0	40.0	12.0	41.0
18-19	33.554252231621035	38.0	32.0	40.5	13.0	41.0
20-21	33.57177979602794	38.0	32.0	40.5	13.0	41.0
22-23	33.52477289978785	38.0	32.0	40.0	13.0	41.0
24-25	33.45165618648623	38.0	32.0	40.5	12.0	41.0
26-27	33.30321381042918	38.0	32.0	40.0	10.0	41.0
28-29	33.22329891750651	38.0	32.0	40.0	10.0	41.0
30-31	33.08896030480647	38.0	32.0	40.0	9.0	41.0
32-33	32.97620990150335	38.0	31.5	40.0	9.0	41.0
34-35	32.79178215575517	38.0	31.0	40.0	8.0	41.0
36-37	32.64131581152181	38.0	31.0	40.0	7.5	41.0
38-39	32.51283666514058	38.0	31.0	40.0	2.0	41.0
40-41	32.42259796555112	38.0	31.0	40.0	2.0	41.0
42-43	32.19972753309031	38.0	30.0	40.0	2.0	41.0
44-45	32.03471685940387	38.0	30.0	40.0	2.0	41.0
46-47	31.828704512884205	37.0	30.0	40.0	2.0	41.0
48-49	31.65820072680854	37.0	30.0	40.0	2.0	41.0
50-51	31.422938745309178	37.0	30.0	40.0	2.0	41.0
52-53	31.235864778843037	37.0	29.5	40.0	2.0	41.0
54-55	31.01428890153128	36.0	29.0	40.0	2.0	41.0
56-57	30.806544257908143	36.0	29.0	40.0	2.0	41.0
58-59	30.533499968149957	35.5	28.5	39.0	2.0	41.0
60-61	30.333790007496525	35.0	28.5	39.0	2.0	41.0
62-63	30.206243769237503	35.0	28.0	39.0	2.0	41.0
64-65	29.966775699453592	35.0	28.0	39.0	2.0	40.5
66-67	29.698616547147946	35.0	28.0	38.5	2.0	40.0
68-69	29.39209692203078	35.0	28.0	38.0	2.0	40.0
70-71	29.061292189145945	34.0	28.0	37.0	2.0	40.0
72-73	28.77664946346016	34.0	27.5	36.5	2.0	39.0
74-75	28.475490372971834	34.0	27.0	36.0	2.0	39.0
76-77	28.195194315065635	34.0	26.5	36.0	2.0	38.5
78-79	27.88357397635064	34.0	26.0	35.0	2.0	37.0
80-81	27.573183865113975	34.0	26.0	35.0	2.0	37.0
82-83	27.314661782365995	33.5	26.0	35.0	2.0	36.5
84-85	27.08228419086302	33.0	25.5	35.0	2.0	36.0
86-87	27.01715415012321	33.0	25.5	35.0	2.0	36.0
88-89	27.110852266326596	34.0	26.0	35.0	2.0	35.5
90-91	27.0289011672805	34.0	26.0	35.0	2.0	35.0
92-93	26.878741399311977	34.0	25.0	35.0	2.0	35.0
94-95	26.798364602334182	34.0	25.0	35.0	2.0	35.0
96-97	26.703337413695017	34.0	25.0	35.0	2.0	35.0
98-99	26.63887873418206	33.5	25.0	35.0	2.0	35.0
100-101	26.46469524844445	33.0	25.0	35.0	2.0	35.0
102-103	25.172785849418652	31.5	21.5	34.0	2.0	35.0
104-105	26.15687008540518	33.0	24.0	35.0	2.0	35.0
106-107	26.338503970586252	33.0	24.0	35.0	2.0	35.0
108-109	26.327670563249946	33.0	24.0	35.0	2.0	35.0
110-111	26.22058146566048	33.0	24.0	35.0	2.0	35.0
112-113	26.122686518081252	33.0	24.0	35.0	2.0	35.0
114-115	25.978879656734996	33.0	23.5	35.0	2.0	35.0
116-117	25.838520601642585	33.0	23.0	35.0	2.0	35.0
118-119	25.639461919431007	33.0	20.0	35.0	2.0	35.0
120-121	25.419001891861107	33.0	18.5	35.0	2.0	35.0
122-123	25.18231011020742	33.0	10.0	35.0	2.0	35.0
124-125	24.043743129883218	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	200051.0
3	27255.0
4	17223.0
5	16232.0
6	19278.0
7	20609.0
8	21086.0
9	22215.0
10	23103.0
11	23828.0
12	23875.0
13	24224.0
14	24887.0
15	22224.0
16	18573.0
17	16729.0
18	15634.0
19	15033.0
20	15196.0
21	16644.0
22	19688.0
23	26516.0
24	35896.0
25	45700.0
26	53564.0
27	59876.0
28	69652.0
29	81404.0
30	94801.0
31	113184.0
32	138631.0
33	171713.0
34	221134.0
35	302243.0
36	460561.0
37	555108.0
38	153077.0
39	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30693450029491	18.31855954200462	12.31666169132209	25.057844266378382
2	16.199897684724583	20.105234085423838	38.53108532654579	25.163782903305787
3	19.379921602746446	24.233501910340635	28.70868631400143	27.677890172911486
4	13.251466580543998	15.818794640433149	35.354736574169536	35.57500220485331
5	14.122083980361314	37.73128242724309	33.821277167329285	14.325356425066312
6	34.034570430300874	35.32862369118204	16.599668492406142	14.037137386110949
7	29.15990410021778	32.13650028556545	20.537616187481564	18.165979426735202
8	26.869328248749618	35.40406059575238	19.290057885675946	18.436553269822063
9	26.821818930379493	16.144944063157123	18.636472183458913	38.396764823004474
10-11	25.523534209339626	26.20241136563404	27.05808811046303	21.21596631456331
12-13	25.947076620653753	23.624374680102644	27.04768043623094	23.380868263012662
14-15	23.232999490386376	25.00988474698813	24.33114781054423	27.42596795208126
16-17	23.47275119581941	28.024254980109685	25.232252401915822	23.270741422155083
18-19	23.56702638391984	27.09144587759195	26.662631281421522	22.678896457066685
20-21	24.42409697080765	26.3208450395724	26.266933317350194	22.988124672269763
22-23	24.010244185085057	26.06421176713134	26.11907900238595	23.80646504539766
24-25	23.67744033880121	26.2381715880883	26.306297090262838	23.778090982847655
26-27	23.613529971802098	26.368254045956363	26.27515013672185	23.743065845519684
28-29	23.60508718464964	26.536414396838627	26.364152284163605	23.494346134348127
30-31	23.828232867908802	26.3963363508106	26.14081100349947	23.634619777781126
32-33	23.618347321465976	26.419943338295976	26.08934612795215	23.872363212285897
34-35	23.862638761860335	26.4332468937694	26.177223229820818	23.526891114549446
36-37	23.91224870346648	26.220825249939278	26.211379368563225	23.655546678031016
38-39	23.80110579414606	26.377092466500844	26.159285004092713	23.66251673526038
40-41	23.82571801228612	26.401165570179053	26.15930599245348	23.613810425081347
42-43	23.83617001814242	26.319953946529207	26.113943428852863	23.729932606475508
44-45	23.72267056947388	26.41726753705795	26.07731474479143	23.782747148676748
46-47	23.692816662748754	26.491508278929803	26.049589780495452	23.766085277825987
48-49	23.682857459797592	26.539181292605544	26.000821848556754	23.77713939904011
50-51	23.665959203553236	26.618380713904866	25.96073187440903	23.754928208132867
52-53	23.798687284724092	26.44121988098546	25.828433231500338	23.931659602790113
54-55	23.714751339546766	26.419324932130312	25.87341775329558	23.992505975027342
56-57	23.74481121588527	26.49644165160585	25.78786284617978	23.970884286329095
58-59	23.724458454511435	26.279472070131227	25.942921878272983	24.05314759708435
60-61	23.737749024330775	26.30972826235327	26.03506290191343	23.91745981140252
62-63	23.624629238194032	26.348593847162423	26.10057983435501	23.926197080288535
64-65	23.70333269470271	26.30237104564859	25.978505293651622	24.015790965997077
66-67	23.68163494055335	26.42353302223825	25.854421055307082	24.04041098190132
68-69	23.866356312560164	26.134608640804345	25.975948341260857	24.023086705374634
70-71	23.653982539712427	26.09710480565859	26.048746963905785	24.200165690723203
72-73	23.830153477285958	26.15818783510881	25.968196732115366	24.04346195548986
74-75	23.70777390254078	26.201012721861417	25.99021858732	24.10099478827781
76-77	23.837165607888736	25.986990281543566	26.115537307899235	24.060306802668467
78-79	23.902475072529754	26.082737345399167	25.97654916984789	24.038238412223187
80-81	23.83930696622148	26.00014215401619	26.058792453410433	24.101758426351903
82-83	23.787584815795608	26.096011653218838	26.06488284805974	24.05152068292582
84-85	23.86458096351327	26.095629594182206	25.86429854633603	24.175490895968498
86-87	23.859592027339822	25.86159145334042	26.08937098422067	24.18944553509909
88-89	23.772118128196578	25.884529006915695	26.080712712936354	24.262640151951373
90-91	23.71837827533484	25.96041317406138	26.116714533500378	24.204494017103404
92-93	23.778383971994767	25.876422680032228	26.130176939148093	24.215016408824912
94-95	23.601177176483585	25.805150806123628	26.258030482569556	24.335641534823235
96-97	23.74123047121081	25.75696882658358	26.209776433010788	24.29202426919482
98-99	23.746586470459484	25.845641390531316	26.0499706991489	24.357801439860296
100-101	23.529953598872687	25.890840445714446	26.165182991797813	24.41402296361505
102-103	23.435859077743736	25.753840616174372	26.380926423762514	24.42937388231938
104-105	23.412072650679047	25.99975961772314	26.10722555859851	24.480942172999296
106-107	23.284747107454617	26.08874134025297	26.140855175322802	24.485656376969608
108-109	23.36716139872324	26.083647732030002	26.15712947033522	24.392061398911537
110-111	23.294150730540792	26.212751693966123	26.09217052537391	24.400927050119176
112-113	23.343710240314227	26.297323881409856	26.048133764978353	24.31083211329756
114-115	23.341655738638707	26.307530633915587	26.041429164802608	24.30938446264309
116-117	23.287176014344897	26.295393452284195	26.08765099769726	24.329779535673648
118-119	23.330345365278703	26.136833455744313	26.0072153701537	24.525605808823286
120-121	23.34332370385259	26.248690401602854	26.01334319346972	24.39464270107484
122-123	23.381385892653974	26.280340261891755	25.96134277699785	24.37693106845642
124-125	23.378504774131805	26.389970790997864	25.751504641948745	24.48001979292158
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1229.0
1	935.5
2	574.0
3	449.0
4	358.0
5	300.5
6	298.5
7	369.5
8	466.0
9	522.5
10	518.5
11	535.5
12	605.5
13	662.0
14	750.0
15	884.5
16	998.0
17	1148.0
18	1373.0
19	1625.5
20	2009.0
21	2603.5
22	3310.0
23	4097.5
24	5173.0
25	6804.5
26	9214.5
27	12430.5
28	15617.5
29	19100.5
30	23922.5
31	29422.5
32	35347.0
33	40379.5
34	44240.5
35	49708.0
36	56922.5
37	63118.5
38	68280.5
39	72939.0
40	78937.0
41	85391.5
42	91025.0
43	98593.5
44	105458.0
45	110579.0
46	116378.0
47	121370.0
48	126191.5
49	130966.0
50	134474.0
51	136108.0
52	134022.0
53	131343.5
54	128550.5
55	123656.0
56	116147.5
57	106481.5
58	97513.5
59	87328.5
60	74475.0
61	61577.0
62	50107.0
63	38892.5
64	28784.5
65	21635.5
66	16473.0
67	12845.5
68	9917.5
69	7579.5
70	5976.5
71	4587.5
72	3535.5
73	2953.5
74	2338.5
75	1749.0
76	1290.0
77	871.0
78	591.5
79	398.5
80	277.5
81	187.5
82	137.0
83	105.0
84	69.5
85	49.5
86	43.5
87	36.5
88	25.5
89	18.5
90	16.0
91	16.5
92	17.5
93	14.5
94	9.5
95	6.5
96	7.5
97	7.5
98	8.0
99	11.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034517286727883595
2	0.018168644559495094
3	0.022844167943544783
4	0.020584854630446944
5	0.013963811449007454
6	0.004863799493474506
7	0.004675523384049687
8	0.005742421337456998
9	0.0028241416413722943
10-11	0.0047069027356204905
12-13	0.007531044376992785
14-15	0.002541727477235065
16-17	0.002573106828805868
18-19	1.5689675785401636E-5
20-21	0.0037027634853547863
22-23	8.001734650554834E-4
24-25	0.0013650017933299421
26-27	0.0014748295238277537
28-29	0.0029967280750117123
30-31	0.001286553414402934
32-33	1.5689675785401636E-5
34-35	0.005946387122667219
36-37	0.007374147619138768
38-39	0.001553277902754762
40-41	0.001333622441759139
42-43	0.002463279098308057
44-45	0.0013493121175445406
46-47	0.018451058723632324
48-49	0.002839831317157696
50-51	0.0096020815806658
52-53	0.0017415540121795815
54-55	1.0982773049781144E-4
56-57	2.3534513678102453E-4
58-59	0.006636732857224891
60-61	6.903457345576719E-4
62-63	0.009272598389172366
64-65	0.009523633201738792
66-67	6.275870314160654E-5
68-69	0.0039694879737066134
70-71	0.004675523384049687
72-73	0.010794496940356325
74-75	0.0015846572543255651
76-77	0.0011767256839051226
78-79	4.236212462058442E-4
80-81	0.003906729270565007
82-83	0.0015689675785401636
84-85	0.0010668979534073112
86-87	0.01237915419468189
88-89	0.0
90-91	5.17759300918254E-4
92-93	0.008205700435765056
94-95	0.006307249665731457
96-97	0.004251902137843843
98-99	0.0013022430901883356
100-101	0.014560019128852719
102-103	0.007640872107490596
104-105	0.006840698642435113
106-107	0.016160366058963687
108-109	0.009507943525953392
110-111	0.017996058125855675
112-113	0.0026829345593036794
114-115	0.013508810851230808
116-117	0.005365869118607359
118-119	0.004377419544127056
120-121	0.007546734052778187
122-123	0.008268459138906663
124-125	0.05748697207771159
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3186809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.70117772553908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.81754851067217	33.3037928986902
2	13.522847547175054	12.901115084063349
3	5.968162406031884	8.540651268750235
4	3.2622022210646113	6.224435516946055
5	1.932166143586674	4.608330030524871
6	1.2637608740865045	3.6169729234429777
7	0.8261604771752676	2.7586179426087534
8	0.6364939984745527	2.4289210673978916
9	0.46609154594248897	2.000980410243654
>10	2.1861211576650263	17.739895254181697
>50	0.08630578815462084	2.767827446943401
>100	0.030807883668982447	2.4856975874204386
>500	9.320124119151775E-4	0.2960591215407133
>1k	3.994338901901035E-4	0.3267034472457628
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.1965546099562288E-4	0.0	0.0	0.0	0.0
3	2.1965546099562288E-4	0.0	0.0	0.0	0.0
4	2.1965546099562288E-4	0.0	0.0	0.0	0.0
5	5.020696251328523E-4	0.0	0.0	0.0	0.0
6	8.472424924116884E-4	0.0	0.0	0.0	0.0
7	0.0017886230395357864	0.0	0.0	0.0	0.0
8	0.001851381742677393	0.0	0.0	0.0	0.0
9	0.0018827610942481962	0.0	0.0	0.0	0.0
10-11	0.002055347527887614	0.0	0.0	1.5689675785401636E-5	0.0
12-13	0.0026672448835182777	0.0	0.0	6.275870314160654E-5	0.0
14-15	0.0032006938602219337	0.0	0.0	6.275870314160654E-5	0.0
16-17	0.0033732802938613517	0.0	0.0	1.4120708206861473E-4	0.0
18-19	0.0042989711652000485	0.0	0.0	1.8827610942481963E-4	0.0
20-21	0.00498931689975772	0.0	0.0	2.5103481256642617E-4	0.0
22-23	0.005428627821748966	0.0	0.0	7.060354103430736E-4	0.0
24-25	0.007185871509713949	0.0	0.0	0.0013806914691153439	0.0
26-27	0.007248630212855555	0.0	0.0	0.0020082785005314093	0.0
28-29	0.00902156357660594	0.0	0.0	0.005820869716384007	0.0
30-31	0.00997863379951544	0.0	0.0	0.01832554131734911	0.0
32-33	0.010135530557369456	0.0	0.0	0.04490385209781948	0.0
34-35	0.011437773647557792	0.0	0.0	0.0805664851580374	0.0
36-37	0.013210707011308177	0.0	0.0	0.14064225374034026	0.0
38-39	0.015077778429770972	1.5689675785401636E-5	0.0	0.23131289010417633	0.0
40-41	0.015218985511839585	3.137935157080327E-5	0.0	0.331773884158103	0.0
42-43	0.016552607953598725	3.137935157080327E-5	0.0	0.42550400730009236	0.0
44-45	0.018137265207924292	3.137935157080327E-5	0.0	0.5333548386489433	0.0
46-47	0.02082019976722797	3.137935157080327E-5	0.0	0.6609432821358293	0.0
48-49	0.021840028693279077	3.137935157080327E-5	0.0	0.7924855239206365	0.0
50-51	0.021840028693279077	3.137935157080327E-5	0.0	0.9315117410550805	0.0
52-53	0.021871408044849883	3.137935157080327E-5	0.0	1.071228303924082	0.0
54-55	0.022043994478489298	3.137935157080327E-5	0.0	1.220688155455818	0.0
56-57	0.02322072016239442	3.137935157080327E-5	0.0	1.3920507943839748	0.0
58-59	0.02331485821710683	3.137935157080327E-5	0.0	1.578161728550409	0.0
60-61	0.023456065299175446	3.137935157080327E-5	0.0	1.773796295918582	0.0
62-63	0.023722789787527274	3.137935157080327E-5	0.0	1.9662458591023184	0.0
64-65	0.02387968654538129	3.137935157080327E-5	0.0	2.1708235416681703	0.0
66-67	0.02529175736606744	3.137935157080327E-5	0.0	2.4149078278616636	0.0
68-69	0.025793826991200287	3.137935157080327E-5	0.0	2.6860568047849744	0.0
70-71	0.02632727596790394	3.137935157080327E-5	0.0	2.9748252876152916	0.0
72-73	0.02697055267510541	3.137935157080327E-5	0.0	3.2660884288954874	0.0
74-75	0.027394173921311257	3.137935157080327E-5	0.0	3.5626076115637932	0.0
76-77	0.027504001651809065	3.137935157080327E-5	0.0	3.8930478732801372	0.0
78-79	0.027598139706521477	3.137935157080327E-5	0.0	4.261394391694012	0.0
80-81	0.03109693740666604	3.137935157080327E-5	0.0	4.651941801344229	0.0
82-83	0.03147348962551568	6.275870314160654E-5	0.0	5.052122671926683	0.0
84-85	0.03219521471164416	6.275870314160654E-5	0.0	5.461011312570035	1.5689675785401636E-5
86-87	0.03307383655562665	6.275870314160654E-5	0.0	5.904636895402266	3.137935157080327E-5
88-89	0.03319935396190986	6.275870314160654E-5	0.0	6.399175476158126	3.137935157080327E-5
90-91	0.03534883954450989	6.275870314160654E-5	0.0	6.926081858059269	3.137935157080327E-5
92-93	0.03613332333377997	6.275870314160654E-5	0.0	7.455969278359638	3.137935157080327E-5
94-95	0.03616470268535077	1.2551740628321308E-4	0.0	8.001420857039125	3.137935157080327E-5
96-97	0.036211771712706974	1.8827610942481963E-4	0.0	8.56817901543519	3.137935157080327E-5
98-99	0.037655221884963924	1.8827610942481963E-4	0.0	9.176514814662568	3.137935157080327E-5
100-101	0.03922418946350409	1.8827610942481963E-4	0.0	9.83491636932116	3.137935157080327E-5
102-103	0.04095005379989827	1.8827610942481963E-4	0.0	10.508458461112667	3.137935157080327E-5
104-105	0.04548437010187934	1.8827610942481963E-4	0.0	11.176195372863576	4.7069027356204907E-5
106-107	0.04686506157099468	1.8827610942481963E-4	0.0	11.871828528160929	6.275870314160654E-5
108-109	0.04732006216877133	1.8827610942481963E-4	0.0	12.59673861847384	6.275870314160654E-5
110-111	0.04785351114547499	1.8827610942481963E-4	0.0	13.358425308827734	6.275870314160654E-5
112-113	0.04832420141903704	1.8827610942481963E-4	0.0	14.153750664065527	6.275870314160654E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8340	0.0	33.96616	1
GTACATG	13835	0.0	30.756186	1
TACATGG	13775	0.0	30.021376	2
TCAACGC	9535	0.0	28.267958	4
CAACGCA	9580	0.0	28.133852	5
CATGGGG	8860	0.0	27.936796	4
ACATGGG	14315	0.0	27.932858	3
TATCAAC	10075	0.0	27.69907	2
AACGCAG	9755	0.0	27.689703	6
ATCAACG	9940	0.0	27.356922	3
GTATAGG	840	0.0	25.505203	1
GGTATCA	6160	0.0	24.925539	1
ACGCAGA	11090	0.0	24.088207	7
CGCAGAG	11015	0.0	23.982153	8
GCAGAGT	12290	0.0	19.848227	9
ATGGGGG	5310	0.0	19.160116	5
GTATATA	1525	0.0	19.121933	1
AGAGTAC	11160	0.0	17.539806	10-11
ATGGGGA	4910	0.0	17.085754	5
CATGGGT	2385	0.0	16.964361	4
>>END_MODULE