FastQCFastQC Report
Mon 15 Aug 2016
SRR2920978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186809
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49840.1563946882288835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39660.12445050832980575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105650.038.97271
GGTATCA72150.036.3386081
TCAACGC128900.031.3322944
CAACGCA129700.031.102385
ATCAACG131500.030.802913
TATCAAC132650.030.7565292
AACGCAG136150.029.7562946
GTACATG135750.025.853981
ACGCAGA156150.025.6410687
CATGGGG86050.025.6133424
CGCAGAG158300.025.3683788
TACATGG137700.024.3084012
ACATGGG138000.024.3017713
GAGTACT82350.022.90481612-13
GCAGAGT172950.022.2910799
CGTAAAC2204.1404383E-621.660873
AGTACTT84150.021.42494412-13
GTACTTT94400.020.01257114-15
AGAGTAC159600.019.8325810-11
CAGAGTA164950.019.31557710-11
Produced by FastQC (version 0.11.5)