FastQCFastQC Report
Mon 15 Aug 2016
SRR2920977_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920977_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4634176
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80200.044.0058061
TCAACGC90650.037.939474
GGTATCA51200.037.197151
CAACGCA93800.036.855295
AACGCAG97650.035.1562046
ATCAACG97750.035.124413
TATCAAC103700.033.625872
GTACATG179750.029.9646591
ACATGGG174750.029.5905173
ACGCAGA117450.028.8242267
TACATGG182250.028.7322622
CGCAGAG122350.027.523668
CATGGGG136450.026.774794
GTATAGG11150.026.688541
GCAGAGT143650.022.3649439
AGAGTAC122350.020.7151310-11
ATGGGGG76850.018.8143375
ATGGGGA73300.018.5079125
GTATCGC3253.2151002E-718.306916
CAGAGTA134100.018.14581910-11
Produced by FastQC (version 0.11.5)