FastQCFastQC Report
Mon 15 Aug 2016
SRR2920977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4634176
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62300.045.707691
GTATCAA99900.044.426311
TCAACGC123250.034.989694
CAACGCA125900.034.2594225
ATCAACG129200.033.4690783
AACGCAG131800.032.7663466
TATCAAC135400.031.8444142
GTACATG173350.028.0080721
ACGCAGA154600.027.398047
ACATGGG171900.027.130363
TACATGG176550.026.9183542
CGCAGAG160950.026.2081938
CATGGGG128900.025.4616834
GCAGAGT183400.022.1897629
AGAGTAC164800.021.53695710-11
CAGAGTA175100.019.47150610-11
ATGGGGA71550.018.3759775
GAGTACT89850.018.1802512-13
GTACTTT93750.017.7095914-15
AGTACTT100700.017.25555812-13
Produced by FastQC (version 0.11.5)