FastQCFastQC Report
Mon 15 Aug 2016
SRR2920976_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920976_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332234
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87090.6537139871824319No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51060.38326600281932455No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT48100.3610476838153057No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG26650.20003993292469643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25280.18975645419648499No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19560.14682105395898917No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC17620.1322590475847336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16640.12490298250907873No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG14580.10944023347249808No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC801.1977175E-437.1867376
CATGGGG41750.030.7860664
TATAGCG450.00953212426.443905116-117
ATGGGGA18800.026.2690645
ATATAGG7700.023.9576512
GTATAGG5550.023.5930711
ACAGACG3553.6379788E-1223.4643088
CTTACTA5650.023.1676679
CATATAG3103.3651304E-1023.0352463
GTACATG135300.023.0069331
GTACATA7850.022.7460731
GTATTAC2903.8980943E-922.5761281
ATGGGGG16900.022.1808515
GAGTACT25850.022.09418512-13
ATGGGAT12450.021.984315
TACATGG139200.021.8451462
CTATACC4101.8189894E-1221.7702984
ACATGGG136450.021.5004063
GAACAAA16400.021.4122831
GGTGTGC5250.020.3995828
Produced by FastQC (version 0.11.5)