##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920976_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1332234
Sequences flagged as poor quality	0
Sequence length	125
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.811214846641054	31.0	28.0	33.0	12.0	34.0
2	27.82898950184427	31.0	28.0	34.0	12.0	34.0
3	27.877037367309345	31.0	27.0	34.0	12.0	34.0
4	31.029012170534607	35.0	32.0	37.0	12.0	37.0
5	31.359004499209597	35.0	32.0	37.0	16.0	37.0
6	31.191846927791964	35.0	32.0	37.0	11.0	37.0
7	31.105197735532947	35.0	32.0	37.0	10.0	37.0
8	31.02296893788929	35.0	32.0	37.0	10.0	37.0
9	31.974795719070375	37.0	32.0	39.0	8.0	39.0
10-11	32.04634883961826	37.0	31.5	39.0	9.0	39.0
12-13	32.039397733431215	37.0	32.0	39.0	9.0	39.0
14-15	32.89787830065889	38.0	32.0	40.0	8.0	41.0
16-17	32.82880672614571	38.0	31.5	40.0	8.0	41.0
18-19	32.96633174052006	38.0	32.0	40.0	8.0	41.0
20-21	33.00143743516529	38.0	32.0	40.0	9.0	41.0
22-23	32.983378670714	38.0	31.5	40.0	10.0	41.0
24-25	32.94536958222054	38.0	31.0	40.0	9.0	41.0
26-27	32.795333627575936	38.0	31.0	40.0	8.0	41.0
28-29	32.71531765440606	38.0	31.0	40.0	7.5	41.0
30-31	32.54840853783945	38.0	30.0	40.0	2.0	41.0
32-33	32.366529078225	38.0	30.0	40.0	2.0	41.0
34-35	32.16122467974846	38.0	30.0	40.0	2.0	41.0
36-37	31.983517535207778	38.0	30.0	40.0	2.0	41.0
38-39	31.804312530681546	38.0	30.0	40.0	2.0	41.0
40-41	31.677976992029926	38.0	30.0	40.0	2.0	41.0
42-43	31.425807328142053	37.0	30.0	40.0	2.0	41.0
44-45	31.229304311404753	37.0	29.5	40.0	2.0	41.0
46-47	30.963540564195178	37.0	29.0	40.0	2.0	41.0
48-49	30.752960065574065	37.0	28.5	40.0	2.0	41.0
50-51	30.528715676074924	36.5	28.0	40.0	2.0	41.0
52-53	30.360864157497858	36.0	28.0	40.0	2.0	41.0
54-55	30.13102765730345	36.0	28.0	40.0	2.0	41.0
56-57	29.907395772814688	35.5	28.0	39.5	2.0	41.0
58-59	29.639932624448857	35.0	27.0	39.0	2.0	41.0
60-61	29.447505092949136	35.0	27.5	39.0	2.0	41.0
62-63	29.287443872472853	35.0	27.0	39.0	2.0	41.0
64-65	29.06335711293962	35.0	26.5	39.0	2.0	40.5
66-67	28.78947542248584	35.0	26.0	38.5	2.0	40.0
68-69	28.465625408149016	34.0	26.0	38.0	2.0	40.0
70-71	28.12012266613823	34.0	26.0	37.0	2.0	40.0
72-73	27.81285194642983	34.0	26.0	37.0	2.0	39.0
74-75	27.491328850637352	34.0	25.5	36.0	2.0	39.0
76-77	27.191617989031958	34.0	25.0	36.0	2.0	38.5
78-79	26.874701816647825	34.0	24.0	35.0	2.0	37.0
80-81	26.553235392581186	33.0	23.5	35.0	2.0	37.0
82-83	26.270236309837458	33.0	23.0	35.0	2.0	36.5
84-85	26.002272123365714	33.0	21.5	35.0	2.0	36.0
86-87	25.916391564845213	33.0	22.0	35.0	2.0	36.0
88-89	25.964464200733506	33.0	23.0	35.0	2.0	35.5
90-91	25.848814097223162	33.0	21.5	35.0	2.0	35.0
92-93	25.665360589806298	33.0	20.0	35.0	2.0	35.0
94-95	25.558231136572104	33.0	20.0	35.0	2.0	35.0
96-97	25.441438215808937	33.0	19.0	35.0	2.0	35.0
98-99	25.35561395370483	33.0	19.0	35.0	2.0	35.0
100-101	25.160308174089536	33.0	17.5	35.0	2.0	35.0
102-103	23.88081147906449	30.5	13.0	34.0	2.0	35.0
104-105	24.805207643702232	32.5	15.0	35.0	2.0	35.0
106-107	24.953136235826438	33.0	15.0	35.0	2.0	35.0
108-109	24.932765940517957	33.0	10.5	35.0	2.0	35.0
110-111	24.805667773078902	33.0	7.0	35.0	2.0	35.0
112-113	24.68229380123912	33.0	4.5	35.0	2.0	35.0
114-115	24.521104400578277	33.0	2.0	35.0	2.0	35.0
116-117	24.357519024435646	33.0	2.0	35.0	2.0	35.0
118-119	24.13991723676171	33.0	2.0	35.0	2.0	35.0
120-121	23.884436217661463	33.0	2.0	35.0	2.0	35.0
122-123	23.62631752379837	33.0	2.0	35.0	2.0	35.0
124-125	22.51224409525654	31.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90926.0
3	11811.0
4	8823.0
5	9830.0
6	12604.0
7	12333.0
8	10812.0
9	11182.0
10	11824.0
11	12484.0
12	12710.0
13	13340.0
14	14135.0
15	12574.0
16	9544.0
17	8398.0
18	7704.0
19	7285.0
20	7180.0
21	7646.0
22	9052.0
23	11789.0
24	16743.0
25	20693.0
26	23025.0
27	24364.0
28	28305.0
29	31895.0
30	37626.0
31	44678.0
32	54707.0
33	67160.0
34	86670.0
35	118019.0
36	177180.0
37	223045.0
38	64101.0
39	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.318563232156976	20.231391827549412	12.044524304927213	23.405520635366397
2	15.167128503850227	22.501518394533328	38.200854505146026	24.130498596470417
3	18.556591895526118	28.340281891479364	28.69262747094257	24.410498742051953
4	11.875807081293733	18.642301570617736	36.047779212588964	33.43411213549957
5	11.943329412056162	41.45583233049235	33.40823047800794	13.192607779443549
6	30.029050346427255	39.41614058265837	16.97523589331692	13.579573177597453
7	26.642340423775064	36.18977772339696	20.396586319003067	16.77129553382491
8	25.81067711187412	37.29107208003855	19.226323003727785	17.671927804359544
9	26.271869015975913	19.032527569203562	18.91993127121971	35.775672143600815
10-11	23.385526450117403	30.053454218987397	26.33346544880225	20.22755388209295
12-13	24.124145877787097	28.27291374608948	26.210371638169338	21.392568737954086
14-15	22.521939256913935	28.677729561830382	23.968868061200972	24.831463120054707
16-17	21.42077286182414	31.48236625833346	25.540072864188645	21.55678801565375
18-19	21.747042936901476	29.95997700103736	27.6448431731963	20.64813688886487
20-21	22.580679061276452	28.936632257212175	27.4078361077082	21.074852573803177
22-23	22.19190524087613	28.764093017669758	26.777146417258415	22.266855324195703
24-25	22.01015228426396	29.35541439522599	26.80672190059769	21.82771141991236
26-27	21.60526099490362	28.984675573357094	27.323612963179116	22.086450468560173
28-29	21.62762671018354	28.706425106975026	27.951060029492492	21.714888153348944
30-31	22.12814780146965	29.108116192564363	27.2194634592873	21.54427254667868
32-33	21.16471280570831	29.339027528196997	27.490966301715762	22.00529336437893
34-35	21.3780357720599	29.442700822800916	27.601626535010514	21.57763687012867
36-37	22.28670023143022	28.778869662673372	26.858554323045965	22.075875782850442
38-39	21.710908542269983	29.408011577649972	26.924191313459765	21.95688856662028
40-41	21.499264014315877	29.358510223858246	27.542115570394536	21.60011019143134
42-43	21.73570363208776	29.190990302572644	26.978574683101524	22.094731382238074
44-45	21.905429602893506	29.09427052272346	27.054073844160648	21.946226030222384
46-47	21.856257866604903	29.43901745940522	27.14599480245695	21.558729871532922
48-49	21.615340616513112	29.558392796287652	26.826299615223515	21.99996697197572
50-51	21.31578325087652	29.720184834377605	26.629940511736443	22.33409140300943
52-53	21.417108285418323	29.77830238248938	26.282933749680982	22.521655582411313
54-55	21.593603218657403	29.62737360032877	26.040912528406267	22.73811065260756
56-57	21.92204510926025	29.52244804208276	25.92953029202121	22.62597655663578
58-59	21.54675292308986	28.760644430965836	26.666814297467557	23.025788348476752
60-61	21.812681791739383	29.450092889714576	26.403835688415995	22.333389630130046
62-63	21.200741525622533	29.186705236808884	26.871595882798715	22.740957354769865
64-65	21.040138547012972	29.0133104523572	26.951057381854817	22.99549361877501
66-67	21.469338122287212	29.12689789507462	26.48531693979842	22.918447042839745
68-69	21.94409028964462	28.4393539565899	26.757100934055394	22.85945481971008
70-71	21.39287797342245	28.637973782763098	27.289842115574732	22.679306128239716
72-73	21.82141528672414	28.306447647513437	27.067079901853514	22.80505716390891
74-75	21.81262512038204	28.627956256375654	26.71317810837123	22.846240514871074
76-77	21.304348152446178	28.40253231258177	27.682118807615254	22.611000727356796
78-79	21.531221511592776	28.297355450126688	27.5187364348737	22.652686603406842
80-81	21.615779772576886	28.48876640019997	27.089412781168402	22.806041046054737
82-83	21.582607448964158	28.521860600926797	27.049774981787994	22.845756968321055
84-85	21.26316595407379	28.410687692159875	26.87911342236741	23.447032931398923
86-87	21.558700312336132	28.130521294672867	27.221814957826552	23.088963435164448
88-89	21.83655423897003	27.90283088406391	27.18306243497764	23.07755244198842
90-91	21.709097419025774	28.115763837987373	27.23393379293883	22.94120495004802
92-93	21.742009122993768	28.205274491162076	27.195874872431602	22.856841513412558
94-95	21.27399718427533	27.851878319558104	27.664288451512824	23.209836044653745
96-97	22.00457521614049	27.651833182890417	27.130130932251895	23.213460668717197
98-99	21.571401817036094	28.0675291589368	26.855271014757648	23.50579800926946
100-101	21.198546579281246	28.46043257283995	27.04015675322628	23.300864094652525
102-103	21.412021789009334	28.552978122786687	26.937193889979238	23.097806198224735
104-105	21.1455642658055	28.792655765467323	26.879428471482502	23.182351497244674
106-107	20.70820118640384	29.22533902749119	26.982898382405217	23.08356140369975
108-109	20.82161762867757	29.590763914616208	26.612563289203084	22.975055167503143
110-111	20.62399774172818	29.582272265899494	26.6062033405606	23.187526651811723
112-113	20.535700546352103	29.724977377637508	26.843201787720233	22.896120288290156
114-115	20.401510280424347	30.17377833981009	26.43981681149759	22.984894568267972
116-117	20.55356737371709	29.862537086837154	26.325358294971952	23.258537244473803
118-119	20.6532448117954	29.052181742639227	26.746938571762612	23.54763487380276
120-121	20.486686751984852	29.663352601676763	26.709144349681214	23.14081629665717
122-123	20.65201153933636	29.633769448983927	26.58033433448011	23.1338846771996
124-125	20.536045216070768	29.639681150244446	26.610330584874575	23.213943048810208
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6869.0
1	4900.5
2	2336.0
3	1423.0
4	934.0
5	724.0
6	659.0
7	730.0
8	933.5
9	931.5
10	796.0
11	731.5
12	749.5
13	825.0
14	914.5
15	1004.0
16	1105.5
17	1316.0
18	1557.0
19	1788.0
20	2111.5
21	2517.5
22	3076.0
23	3672.5
24	4281.5
25	5524.5
26	8045.0
27	10872.0
28	11727.0
29	12902.0
30	16249.0
31	19779.5
32	24514.0
33	29285.0
34	34166.0
35	38524.0
36	42477.5
37	46155.5
38	44968.0
39	42114.5
40	39816.0
41	38196.0
42	37264.0
43	38438.5
44	40530.0
45	41197.0
46	41474.5
47	40743.0
48	41242.5
49	44718.5
50	52552.5
51	52560.5
52	44061.0
53	40451.5
54	39607.0
55	37746.0
56	34840.5
57	31474.5
58	28949.5
59	26134.5
60	22618.5
61	19900.5
62	17061.0
63	12980.5
64	8988.5
65	6645.5
66	4928.5
67	3652.0
68	2831.0
69	2277.0
70	1842.0
71	1384.5
72	1031.5
73	868.5
74	704.5
75	500.0
76	366.5
77	248.0
78	156.0
79	122.5
80	97.5
81	67.5
82	50.0
83	36.5
84	21.0
85	11.5
86	12.5
87	10.0
88	7.0
89	7.5
90	6.5
91	7.5
92	6.5
93	2.0
94	2.0
95	2.5
96	2.0
97	2.0
98	4.0
99	5.5
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03505390194215131
2	0.01763954380386629
3	0.022743752223708447
4	0.02056695745642282
5	0.013886449377511757
6	0.0048039608657337975
7	0.0048790227542608875
8	0.005704703528058885
9	0.0030775374296107137
10-11	0.004954084642787979
12-13	0.006868162800228788
14-15	0.0024770423213939896
16-17	0.0022143257115491725
18-19	0.0
20-21	0.003152599318137805
22-23	0.0010508664393792683
24-25	0.0016138306033324475
26-27	0.0010883973836428133
28-29	0.002289387600076263
30-31	0.0010883973836428133
32-33	0.0
34-35	0.005779765416585975
36-37	0.006230136747748518
38-39	9.00742662325087E-4
40-41	5.254332196896342E-4
42-43	0.0018765472131772646
44-45	7.130879410073606E-4
46-47	0.019403498184252917
48-49	0.0024019804328668987
50-51	0.009195081344568597
52-53	0.0010508664393792683
54-55	1.1259283279063587E-4
56-57	1.5012377705418117E-4
58-59	0.0061550748592214275
60-61	6.755569967438152E-4
62-63	0.008669648124878962
64-65	0.009232612288832142
66-67	1.1259283279063587E-4
68-69	0.0024395113771304437
70-71	0.004278527646044163
72-73	0.010771381003637498
74-75	0.00127605210496054
76-77	9.758045508521776E-4
78-79	2.627166098448171E-4
80-81	0.003565439705036803
82-83	6.3802605248027E-4
84-85	7.506188852709059E-4
86-87	0.013173361436504398
88-89	0.0
90-91	3.377784983719076E-4
92-93	0.007919029239608057
94-95	0.0054419869182140675
96-97	0.004240996701780618
98-99	7.130879410073606E-4
100-101	0.015312625259526478
102-103	0.007093348465810061
104-105	0.006530384301856881
106-107	0.016926455862858927
108-109	0.011597061777435495
110-111	0.01816497702355592
112-113	0.0025896351541846254
114-115	0.012272618774179312
116-117	0.004240996701780618
118-119	0.004015811036199346
120-121	0.006267667692012064
122-123	0.007130879410073606
124-125	0.05708456622485239
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1332234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.49602227669081
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.73509980219085	29.517391666380398
2	10.298840237240212	8.13526446868238
3	4.62845709984734	5.4841693416683475
4	2.5759934276536263	4.069659752128668
5	1.6833136119432601	3.3242095957983935
6	1.1304628502193703	2.6789271549141374
7	0.7941392734127313	2.1955739703453
8	0.64143811774493	2.02674033500579
9	0.45878004848795967	1.630798831366357
>10	2.679588916801527	19.48815672804936
>50	0.2219893746104385	6.060772519290858
>100	0.1410057623493129	10.507211579476545
>500	0.007974117454226923	2.011275375440963
>1k	0.0025283787049987802	1.808634561156379
>5k	3.889813392305816E-4	1.0612141202962573
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8709	0.6537139871824319	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	5106	0.38326600281932455	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	4810	0.3610476838153057	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	2665	0.20003993292469643	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2528	0.18975645419648499	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	1956	0.14682105395898917	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	1762	0.1322590475847336	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1664	0.12490298250907873	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	1458	0.10944023347249808	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.506188852709059E-5	0.0
3	0.0	0.0	0.0	7.506188852709059E-5	0.0
4	0.0	0.0	0.0	7.506188852709059E-5	0.0
5	0.0	0.0	0.0	7.506188852709059E-5	0.0
6	0.0	0.0	0.0	7.506188852709059E-5	0.0
7	0.0	0.0	0.0	7.506188852709059E-5	0.0
8	0.0	0.0	0.0	7.506188852709059E-5	0.0
9	0.0	0.0	0.0	1.5012377705418117E-4	0.0
10-11	0.0	0.0	0.0	1.5012377705418117E-4	0.0
12-13	0.0	0.0	0.0	2.2518566558127177E-4	0.0
14-15	0.0	0.0	0.0	2.2518566558127177E-4	0.0
16-17	0.0	0.0	0.0	4.1284038689899823E-4	0.0
18-19	0.0	0.0	0.0	7.506188852709058E-4	0.0
20-21	0.0	0.0	0.0	0.00127605210496054	0.0
22-23	0.0	0.0	0.0	0.002664697042711716	0.0
24-25	0.0	0.0	0.0	0.005291863141159887	0.0
26-27	0.0	0.0	0.0	0.00821927679371642	0.0
28-29	0.0	0.0	0.0	0.01771460569239338	0.0
30-31	0.0	0.0	0.0	0.0425976217391239	0.0
32-33	0.0	0.0	0.0	0.08707179069142508	0.0
34-35	0.0	0.0	0.0	0.1443440116375952	0.0
36-37	0.0	0.0	0.0	0.2329545710438256	0.0
38-39	0.0	0.0	0.0	0.38270303865537136	0.0
40-41	0.0	0.0	0.0	0.5627389782875981	0.0
42-43	0.0	0.0	0.0	0.7056192830989151	0.0
44-45	0.0	0.0	0.0	0.8557055292088327	0.0
46-47	0.0	0.0	0.0	1.0326639314114487	0.0
48-49	0.0	0.0	0.0	1.2246722422637464	0.0
50-51	0.0	0.0	0.0	1.4419764095496737	0.0
52-53	0.0	0.0	0.0	1.6541763684157589	0.0
54-55	3.753094426354529E-5	0.0	0.0	1.8639368159047134	0.0
56-57	7.506188852709059E-5	0.0	0.0	2.095164963512416	0.0
58-59	7.506188852709059E-5	0.0	0.0	2.3588949088523488	0.0
60-61	7.506188852709059E-5	0.0	0.0	2.649234293675135	0.0
62-63	7.506188852709059E-5	0.0	0.0	2.9218215418612647	0.0
64-65	7.506188852709059E-5	0.0	0.0	3.2011268290705686	0.0
66-67	7.506188852709059E-5	0.0	0.0	3.5063659987659825	0.0
68-69	7.506188852709059E-5	0.0	0.0	3.8567173634661778	0.0
70-71	7.506188852709059E-5	0.0	0.0	4.27965357437207	0.0
72-73	7.506188852709059E-5	0.0	0.0	4.675755160129526	0.0
74-75	7.506188852709059E-5	0.0	0.0	5.043558413912271	0.0
76-77	7.506188852709059E-5	0.0	0.0	5.448930142902824	0.0
78-79	7.506188852709059E-5	0.0	0.0	5.912850144944507	0.0
80-81	7.506188852709059E-5	0.0	0.0	6.416177638462912	0.0
82-83	7.506188852709059E-5	0.0	0.0	6.947390623569133	0.0
84-85	7.506188852709059E-5	0.0	0.0	7.447753172490719	0.0
86-87	7.506188852709059E-5	0.0	0.0	7.948491030854941	0.0
88-89	7.506188852709059E-5	0.0	0.0	8.513106556355716	0.0
90-91	7.506188852709059E-5	0.0	0.0	9.122083657976003	0.0
92-93	7.506188852709059E-5	0.0	0.0	9.768404049138514	0.0
94-95	7.506188852709059E-5	0.0	0.0	10.401288362254679	0.0
96-97	7.506188852709059E-5	0.0	0.0	11.037475398466036	0.0
98-99	7.506188852709059E-5	0.0	0.0	11.730334160515344	0.0
100-101	7.506188852709059E-5	0.0	0.0	12.52171165125646	0.0
102-103	7.506188852709059E-5	0.0	0.0	13.315716308096025	0.0
104-105	7.506188852709059E-5	0.0	0.0	14.08814067198405	0.0
106-107	7.506188852709059E-5	0.0	0.0	14.830089909130077	0.0
108-109	7.506188852709059E-5	0.0	0.0	15.58607571943067	0.0
110-111	7.506188852709059E-5	0.0	0.0	16.42586812827176	0.0
112-113	7.506188852709059E-5	0.0	0.0	17.32983845180351	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGC	80	1.1977175E-4	37.186737	6
CATGGGG	4175	0.0	30.786066	4
TATAGCG	45	0.009532124	26.443905	116-117
ATGGGGA	1880	0.0	26.269064	5
ATATAGG	770	0.0	23.957651	2
GTATAGG	555	0.0	23.593071	1
ACAGACG	355	3.6379788E-12	23.464308	8
CTTACTA	565	0.0	23.167667	9
CATATAG	310	3.3651304E-10	23.035246	3
GTACATG	13530	0.0	23.006933	1
GTACATA	785	0.0	22.746073	1
GTATTAC	290	3.8980943E-9	22.576128	1
ATGGGGG	1690	0.0	22.180851	5
GAGTACT	2585	0.0	22.094185	12-13
ATGGGAT	1245	0.0	21.98431	5
TACATGG	13920	0.0	21.845146	2
CTATACC	410	1.8189894E-12	21.770298	4
ACATGGG	13645	0.0	21.500406	3
GAACAAA	1640	0.0	21.412283	1
GGTGTGC	525	0.0	20.399582	8
>>END_MODULE