FastQCFastQC Report
Mon 15 Aug 2016
SRR2920976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332234
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89910.6748814397470715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67140.5039655195708862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57470.4313806733651896No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT51130.38379143603901417No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46240.3470861725492669No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23270.17466901460253978No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21410.16070750333650094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19420.14577018751960993No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC17540.1316585524765169No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17460.13105805736830017No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16500.12385211606969945No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13870.10411083938707463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147350.030.9816361
ACCGACT850.00721020628.0004528
GACATAT3950.027.1582091
CATGGGG39500.027.1520844
CATATAG3007.2759576E-1225.818653
GAGTACT94400.025.7480312-13
AGTACTT100250.025.49193412-13
TATCAAC176850.025.3977972
CTGTATA2851.1641532E-1025.087894
TCAACGC178400.025.0158184
ATCAACG180500.024.9549083
GTATAGA2401.2798409E-824.832161
AACGCAG179900.024.769056
CAACGCA179700.024.6641945
GGTATCA108750.024.058081
CATGGGT9600.023.5852284
GTACTTT103250.023.48342914-15
GCGTGTC1300.002052137922.8849839
TAGACAG3950.022.5919065
ACGCAGA197500.022.3542827
Produced by FastQC (version 0.11.5)