##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920976_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1332234 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.706249802962542 31.0 30.0 34.0 26.0 34.0 2 29.72131472398993 31.0 30.0 34.0 26.0 34.0 3 29.833315318480086 31.0 30.0 34.0 26.0 34.0 4 33.37584988823285 35.0 33.0 37.0 28.0 37.0 5 32.93175823466448 35.0 33.0 37.0 26.0 37.0 6 32.69856796929068 35.0 33.0 37.0 25.0 37.0 7 32.51663446511649 35.0 33.0 37.0 25.0 37.0 8 32.44489856887003 35.0 33.0 37.0 23.0 37.0 9 33.63545293094156 37.0 33.0 39.0 23.0 39.0 10-11 33.57488474247017 37.0 33.0 39.0 22.5 39.0 12-13 33.48684878182061 37.0 33.0 39.0 22.0 39.0 14-15 34.27152812493901 38.0 33.0 41.0 19.0 41.0 16-17 34.3001000574974 38.0 33.0 40.5 20.5 41.0 18-19 34.44642270051658 38.5 33.5 41.0 21.5 41.0 20-21 34.452658466905966 39.0 33.0 41.0 21.5 41.0 22-23 34.39946098057848 39.0 33.0 41.0 22.0 41.0 24-25 34.35254617432073 39.0 33.0 41.0 21.5 41.0 26-27 34.143240977185684 38.0 33.0 41.0 19.0 41.0 28-29 33.966208263713426 38.0 33.0 40.5 18.0 41.0 30-31 33.67351681461365 38.0 33.0 40.0 16.5 41.0 32-33 33.36861767527326 38.0 32.0 40.0 16.0 41.0 34-35 33.148332425084476 38.0 32.0 40.0 12.0 41.0 36-37 32.9056062973922 38.0 32.0 40.0 9.0 41.0 38-39 32.69647449321966 38.0 32.0 40.0 8.0 41.0 40-41 32.42951913852972 38.0 31.0 40.0 2.0 41.0 42-43 32.172914442958216 38.0 31.0 40.0 2.0 41.0 44-45 31.789315165353834 37.0 30.0 40.0 2.0 41.0 46-47 31.39102177245139 37.0 30.0 40.0 2.0 41.0 48-49 30.993135965603642 37.0 29.5 40.0 2.0 41.0 50-51 30.744895416270715 37.0 29.0 40.0 2.0 41.0 52-53 30.516206612351883 36.5 28.0 40.0 2.0 41.0 54-55 30.30553153575123 36.0 28.0 40.0 2.0 41.0 56-57 30.14382458336899 36.0 28.0 40.0 2.0 41.0 58-59 30.062745733857568 36.0 28.0 40.0 2.0 41.0 60-61 29.799714990009264 35.0 28.0 39.5 2.0 41.0 62-63 29.51498798259165 35.0 27.0 39.0 2.0 41.0 64-65 29.26324241837395 35.0 27.0 39.0 2.0 41.0 66-67 28.94798511372627 35.0 26.0 38.5 2.0 40.5 68-69 28.625157067001744 35.0 26.0 38.0 2.0 40.0 70-71 28.274563627711046 34.0 26.0 37.0 2.0 40.0 72-73 27.992664952253133 34.0 26.0 37.0 2.0 39.0 74-75 27.7260327389933 34.0 26.0 36.0 2.0 39.0 76-77 27.39913633791061 34.0 25.0 36.0 2.0 38.5 78-79 27.09223905109763 34.0 25.0 35.0 2.0 37.0 80-81 26.845831137773093 34.0 24.5 35.0 2.0 37.0 82-83 26.59785105319336 34.0 24.0 35.0 2.0 36.5 84-85 26.394245680563625 33.5 24.0 35.0 2.0 36.0 86-87 26.17975896126356 33.0 23.0 35.0 2.0 36.0 88-89 26.033171725087335 33.0 22.5 35.0 2.0 35.5 90-91 25.84336985844829 33.0 20.5 35.0 2.0 35.0 92-93 25.95129384177254 33.0 23.0 35.0 2.0 35.0 94-95 25.884996554659317 33.0 21.5 35.0 2.0 35.0 96-97 25.813864906615503 33.0 20.5 35.0 2.0 35.0 98-99 25.752033051250756 33.0 20.0 35.0 2.0 35.0 100-101 25.618241990521184 33.0 20.0 35.0 2.0 35.0 102-103 24.369561953830935 31.0 18.5 34.0 2.0 35.0 104-105 25.34851647683515 33.0 19.0 35.0 2.0 35.0 106-107 25.508533410797202 33.0 19.0 35.0 2.0 35.0 108-109 25.48412290933875 33.0 19.0 35.0 2.0 35.0 110-111 25.40638506448567 33.0 18.5 35.0 2.0 35.0 112-113 25.286054101606773 33.0 17.5 35.0 2.0 35.0 114-115 25.17330026106525 33.0 15.0 35.0 2.0 35.0 116-117 25.0208000246203 33.0 8.5 35.0 2.0 35.0 118-119 24.870246142944858 33.0 2.0 35.0 2.0 35.0 120-121 24.708601116620656 33.0 2.0 35.0 2.0 35.0 122-123 24.459324713226057 33.0 2.0 35.0 2.0 35.0 124-125 23.396434860542517 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51968.0 3 16017.0 4 7910.0 5 7059.0 6 8761.0 7 10793.0 8 11532.0 9 12362.0 10 13924.0 11 15141.0 12 16824.0 13 19807.0 14 23506.0 15 17894.0 16 10433.0 17 8811.0 18 8029.0 19 7669.0 20 7304.0 21 7590.0 22 8115.0 23 10289.0 24 13310.0 25 16984.0 26 20283.0 27 23332.0 28 27051.0 29 31859.0 30 36740.0 31 43870.0 32 53184.0 33 65968.0 34 84259.0 35 115916.0 36 178373.0 37 242087.0 38 77195.0 39 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62105489243654 20.505096929526147 13.517940260711663 23.355907917325656 2 15.275030719100938 22.221938654874933 37.82266315276682 24.680367473257306 3 18.62286546849912 27.033308036850297 30.581346208267373 23.762480286383212 4 12.098587653845923 17.818243856685044 36.43217251185974 33.65099597760929 5 11.526591308173296 39.65787634390439 34.62128941249954 14.194242935422771 6 30.204744339805345 38.76225606103597 16.618100312416022 14.41489928674266 7 25.862804090775338 34.821433933257666 21.607334810624483 17.70842716534251 8 26.896814207921015 34.52798659562106 19.77768969643466 18.79750950002327 9 26.627619661851238 15.282089708003385 20.321210542240816 37.76908008790456 10-11 24.087779208839937 27.29340432169093 27.34487000504146 21.273946464427677 12-13 25.613024705678317 25.40359665185392 27.96658460720697 21.01679403526079 14-15 23.259251194762193 25.602899715059074 26.78778846045553 24.350060629723206 16-17 21.106670396116368 28.735885503391234 28.058765114126793 22.09867898636561 18-19 21.057348511329618 26.19420841723245 32.19908861436267 20.549354457075253 20-21 21.571355315920545 25.40747169714993 32.33248218941944 20.688690797510084 22-23 21.24688324237394 25.38852012105896 31.83447417361344 21.53012246295366 24-25 21.178968074463512 26.035876577366263 31.759611540417925 21.025543807752296 26-27 20.73623077203176 25.752460778266993 32.31158308535665 21.199725364344587 28-29 20.68515566139906 25.219950816305925 33.00172567120802 21.093167851087 30-31 21.146192235585783 25.503360995632544 32.391130845703536 20.959315923078137 32-33 20.292236983523896 25.838342786631262 32.43974672244169 21.429673507403155 34-35 20.61053513614166 25.94124855088569 32.54001207344982 20.908204239522828 36-37 21.612614388286637 25.30344924679713 31.564278004598993 21.519658360317237 38-39 21.09445627460933 25.896072418629657 31.55362768919337 21.455843617567645 40-41 20.84780805773946 25.823271264563736 32.06970694385053 21.259213733846284 42-43 21.357717210200562 25.681760672889283 31.270887942640424 21.689634174269735 44-45 21.586335393000486 25.597111385332955 31.06266886761605 21.753884354050516 46-47 21.842299017846518 25.98000400235335 30.526279429593313 21.65141755020682 48-49 21.725775049011915 26.478785780950716 29.669121704976426 22.126317465060943 50-51 21.440753077414847 26.78010678557257 29.303153376531576 22.475986760481003 52-53 21.468647428434775 26.97218031083959 29.033719777346377 22.525452483379254 54-55 21.54399938730183 26.922490678127726 28.621902157508483 22.911607777061963 56-57 21.899497630808572 26.86901836414296 28.391367448788902 22.840116556259566 58-59 21.276367300417913 26.30509408660683 29.22316042701249 23.195378185962767 60-61 21.704385542023918 27.07029542822901 28.7391097354197 22.486209294327374 62-63 21.09675425595789 26.939236219978678 29.183196283797862 22.780813240265566 64-65 20.883675577364322 26.637663126142126 29.317555727686116 23.16110556880744 66-67 21.316944675137826 26.821685111290993 28.778156899200592 23.08321331437059 68-69 21.711122540535875 26.192650045613412 28.89671846198319 23.19950895186752 70-71 21.242944004445256 26.31969408845079 29.424538626589303 23.012823280514656 72-73 21.747197798938366 25.99512210140112 28.991507981026054 23.266172118634458 74-75 21.599215261997905 26.356633089560518 28.55370428696544 23.490447361476136 76-77 21.00787907613337 26.148179486322825 29.507813945840876 23.336127491702925 78-79 21.100638103851335 26.264522709807096 29.152264561002795 23.48257462533877 80-81 21.051592306953054 26.46947527749748 28.680783299697275 23.798149115852194 82-83 20.997960182063043 26.433269644558067 28.57611096489433 23.99265920848456 84-85 20.66801958350883 26.344188951879854 28.454686670615803 24.533104793995513 86-87 20.90310615204696 26.129671101166284 28.731390212814684 24.235832533972072 88-89 20.986514592922415 25.918712128606934 28.757029906668368 24.337743371802283 90-91 20.91780993693398 26.1026041107421 28.768173998839146 24.211411953484774 92-93 20.757291700296797 26.27688752307809 28.727576055397925 24.238244721227186 94-95 20.406965067674225 25.827585106602623 29.131130894395923 24.634318931327225 96-97 21.204555606857433 25.551338791352755 28.610645014771187 24.63346058701862 98-99 20.842199074108834 25.727078337360144 28.405195261527467 25.025527327003555 100-101 20.51411131382751 26.025744982769105 28.504516070905694 24.95562763249769 102-103 20.717179335378326 25.756070128439664 28.419317043211034 25.107433492970976 104-105 20.537163944057465 25.76416066491354 28.44708978322362 25.251585607805378 106-107 20.069511072673194 26.117514928476943 28.563840728879043 25.249133269970827 108-109 20.19850719824143 26.367650856761905 28.225582435930548 25.20825950906612 110-111 19.944396706897592 26.17671801675081 28.25433363702672 25.624551639324878 112-113 19.73423480742515 26.255282778564165 28.4709436387257 25.53953877528499 114-115 19.616290409399838 26.6468371792379 27.99872825018787 25.738144161174393 116-117 19.65617773146669 26.363373270387964 28.03849241904141 25.94195657910393 118-119 19.65064404036522 25.746901407287687 28.408564584573508 26.193889967773586 120-121 19.418203791940577 26.483794267612744 28.280527786483304 25.81747415396337 122-123 19.40997617820668 26.60102756114304 28.001134978777326 25.987861281872952 124-125 19.155196584643893 26.520121511328128 28.111005082249978 26.213676821778005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 545.0 1 412.5 2 655.0 3 1181.5 4 897.0 5 527.5 6 999.0 7 2122.5 8 3263.0 9 3371.0 10 2345.5 11 1433.0 12 1212.5 13 1250.0 14 1342.0 15 1464.0 16 1592.0 17 1719.0 18 1952.0 19 2206.0 20 2458.5 21 2858.5 22 3331.0 23 3753.5 24 4211.5 25 5342.0 26 7936.5 27 10696.0 28 11486.5 29 12951.0 30 16388.5 31 20015.0 32 25221.0 33 29797.5 34 34030.0 35 38404.5 36 42348.5 37 45807.5 38 44887.0 39 42009.0 40 39451.5 41 37716.0 42 36981.5 43 38502.5 44 40668.5 45 41237.0 46 41386.0 47 41254.0 48 41775.5 49 44623.5 50 53849.0 51 53946.0 52 43985.0 53 40142.0 54 39248.0 55 37609.0 56 34805.5 57 31362.5 58 28708.5 59 26022.0 60 22532.0 61 19506.0 62 16679.5 63 12713.5 64 8549.0 65 6156.5 66 4582.0 67 3363.0 68 2530.5 69 1986.5 70 1592.5 71 1215.0 72 884.0 73 705.5 74 564.5 75 405.0 76 299.5 77 175.0 78 95.0 79 68.5 80 60.0 81 46.0 82 34.5 83 21.0 84 9.5 85 4.5 86 3.0 87 2.5 88 1.5 89 2.0 90 2.0 91 3.0 92 2.5 93 0.5 94 2.0 95 5.0 96 4.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0486401037655547 2 7.506188852709059E-5 3 0.0024770423213939896 4 0.12625409650256636 5 0.015087439593945207 6 0.002702227986975261 7 0.004954084642787979 8 0.009908169285575958 9 0.025145732656575347 10-11 0.02090473595479473 12-13 0.02049189556789573 14-15 0.02844845575176733 16-17 0.03689291821106502 18-19 0.0394450224209861 20-21 0.022593628446654267 22-23 0.02409486621719608 24-25 0.023006468833553267 26-27 0.021580292951538543 28-29 0.021655354840065633 30-31 0.025295856433629527 32-33 0.025183263600838893 34-35 0.028973888971456963 36-37 0.031638586014168676 38-39 0.03167611695843223 40-41 0.03441587588967104 42-43 0.0319013026240135 44-45 0.02886129613866633 46-47 0.03884452731276938 48-49 0.031150683738742593 50-51 0.023456840164715807 52-53 0.029236605581301783 54-55 0.03152599318137805 56-57 0.025896351541846253 58-59 0.02822327008618606 60-61 0.04053341980462891 62-63 0.04950331548361624 64-65 0.041847002853853 66-67 0.03753094426354529 68-69 0.02919907463703824 70-71 0.03576698988315866 72-73 0.038506748814397466 74-75 0.024357582827040897 76-77 0.021655354840065633 78-79 0.029837100689518507 80-81 0.024770423213939893 82-83 0.03734328954222756 84-85 0.022293380892545903 86-87 0.03313982378471049 88-89 0.03182624073548641 90-91 0.03415315927982621 92-93 0.025746227764792073 94-95 0.02263115939091781 96-97 0.018690410243245557 98-99 0.02462029943688571 100-101 0.02432005188277735 102-103 0.027284996479597427 104-105 0.02488301604673053 106-107 0.016213367921851567 108-109 0.01816497702355592 110-111 0.02240597372533654 112-113 0.014524475429992028 114-115 0.015838058479216113 116-117 0.01347360899061276 118-119 0.007281003187127787 120-121 0.004991615587051524 122-123 0.0037906253706180746 124-125 0.06271420786438418 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1332234.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.544260088828814 #Duplication Level Percentage of deduplicated Percentage of total 1 72.50123297237032 26.495039145030724 2 11.076471087635644 8.095628805858993 3 4.9923714036182005 5.473275571015647 4 2.9149061608205353 4.260923555022205 5 1.7971703220788342 3.2838129836986587 6 1.2181686328823498 2.671024281126337 7 0.8934131457491253 2.285438565552433 8 0.676891425061572 1.9789197031478454 9 0.5232114363954711 1.7208337331777228 >10 2.9748440824843505 19.95365069752314 >50 0.25718904270406623 6.514827161147845 >100 0.16078377646620035 11.055963205973848 >500 0.009384266062500536 2.1928413450430497 >1k 0.003128088687500179 1.9933258574041153 >5k 8.34156983333381E-4 2.024495389277425 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8991 0.6748814397470715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6714 0.5039655195708862 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5747 0.4313806733651896 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5113 0.38379143603901417 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4624 0.3470861725492669 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2327 0.17466901460253978 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2141 0.16070750333650094 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1942 0.14577018751960993 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1754 0.1316585524765169 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1746 0.13105805736830017 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1650 0.12385211606969945 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1387 0.10411083938707463 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.506188852709059E-5 0.0 3 0.0 0.0 0.0 7.506188852709059E-5 0.0 4 0.0 0.0 0.0 7.506188852709059E-5 0.0 5 0.0 0.0 0.0 7.506188852709059E-5 0.0 6 0.0 0.0 0.0 7.506188852709059E-5 0.0 7 0.0 0.0 0.0 7.506188852709059E-5 0.0 8 0.0 0.0 0.0 7.506188852709059E-5 0.0 9 0.0 0.0 0.0 1.5012377705418117E-4 0.0 10-11 0.0 0.0 0.0 2.2518566558127177E-4 0.0 12-13 0.0 0.0 0.0 3.0024755410836234E-4 0.0 14-15 0.0 0.0 0.0 3.0024755410836234E-4 0.0 16-17 0.0 0.0 0.0 4.879022754260888E-4 0.0 18-19 0.0 0.0 0.0 8.256807737979965E-4 0.0 20-21 0.0 0.0 0.0 0.0013511139934876306 0.0 22-23 0.0 0.0 0.0 0.0027772898755023517 0.0 24-25 0.0 0.0 0.0 0.005329394085423432 0.0 26-27 0.0 0.0 0.0 0.008256807737979965 0.0 28-29 0.0 0.0 0.0 0.01835263174487365 0.0 30-31 0.0 0.0 0.0 0.043498364401449 0.0 32-33 0.0 0.0 0.0 0.08729697635700635 0.0 34-35 0.0 0.0 0.0 0.1435933927523243 0.0 36-37 0.0 0.0 0.0 0.23100296194212128 0.0 38-39 0.0 0.0 0.0 0.378274387232273 0.0 40-41 0.0 0.0 0.0 0.55598340832016 0.0 42-43 0.0 0.0 0.0 0.6966493874199278 0.0 44-45 0.0 0.0 0.0 0.8441084674313972 0.0 46-47 0.0 0.0 0.0 1.0189276058109913 0.0 48-49 0.0 0.0 0.0 1.2099225811681733 0.0 50-51 0.0 0.0 0.0 1.4240366181916992 0.0 52-53 0.0 0.0 0.0 1.634022251346235 0.0 54-55 0.0 0.0 0.0 1.842431584841702 0.0 56-57 0.0 0.0 0.0 2.0754612177740546 0.0 58-59 0.0 0.0 0.0 2.3394538797238322 0.0 60-61 0.0 0.0 0.0 2.626903381838326 0.0 62-63 0.0 0.0 0.0 2.8988150730277114 0.0 64-65 0.0 0.0 0.0 3.177144555686163 0.0 66-67 0.0 0.0 0.0 3.480394585335609 0.0 68-69 0.0 0.0 0.0 3.8291321194324723 0.0 70-71 0.0 0.0 0.0 4.250679685400613 0.0 72-73 0.0 0.0 0.0 4.644154105059622 0.0 74-75 0.0 0.0 0.0 5.010906492402986 0.0 76-77 0.0 0.0 0.0 5.414289081347571 0.0 78-79 0.0 0.0 0.0 5.874681174628481 0.0 80-81 0.0 0.0 0.0 6.3769578017075075 0.0 82-83 0.0 0.0 0.0 6.90554362071528 0.0 84-85 0.0 0.0 0.0 7.400051342331753 0.0 86-87 0.0 0.0 0.0 7.895722523220396 0.0 88-89 0.0 0.0 0.0 8.456584954294815 0.0 90-91 0.0 0.0 0.0 9.063047482649445 0.0 92-93 0.0 0.0 0.0 9.706703176769246 0.0 94-95 0.0 0.0 0.0 10.340976134823162 0.0 96-97 0.0 0.0 0.0 10.979189842024748 7.506188852709059E-5 98-99 0.0 0.0 0.0 11.673362187123283 7.506188852709059E-5 100-101 7.506188852709059E-5 0.0 0.0 12.470069071949823 7.506188852709059E-5 102-103 7.506188852709059E-5 0.0 0.0 13.266813487720626 7.506188852709059E-5 104-105 7.506188852709059E-5 0.0 0.0 14.042690698480897 7.506188852709059E-5 106-107 7.506188852709059E-5 0.0 0.0 14.789181179882814 7.506188852709059E-5 108-109 7.506188852709059E-5 0.0 0.0 15.555000097580454 7.506188852709059E-5 110-111 7.506188852709059E-5 0.0 0.0 16.40481326853991 7.506188852709059E-5 112-113 7.506188852709059E-5 0.0 0.0 17.319780158740883 7.506188852709059E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14735 0.0 30.981636 1 ACCGACT 85 0.007210206 28.000452 8 GACATAT 395 0.0 27.158209 1 CATGGGG 3950 0.0 27.152084 4 CATATAG 300 7.2759576E-12 25.81865 3 GAGTACT 9440 0.0 25.74803 12-13 AGTACTT 10025 0.0 25.491934 12-13 TATCAAC 17685 0.0 25.397797 2 CTGTATA 285 1.1641532E-10 25.08789 4 TCAACGC 17840 0.0 25.015818 4 ATCAACG 18050 0.0 24.954908 3 GTATAGA 240 1.2798409E-8 24.83216 1 AACGCAG 17990 0.0 24.76905 6 CAACGCA 17970 0.0 24.664194 5 GGTATCA 10875 0.0 24.05808 1 CATGGGT 960 0.0 23.585228 4 GTACTTT 10325 0.0 23.483429 14-15 GCGTGTC 130 0.0020521379 22.884983 9 TAGACAG 395 0.0 22.591906 5 ACGCAGA 19750 0.0 22.354282 7 >>END_MODULE