FastQCFastQC Report
Mon 15 Aug 2016
SRR2920975_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920975_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3246980
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61200.039.1869351
TCAACGC68950.032.7921644
ATCAACG69450.032.6412543
CAACGCA69550.032.422725
TATCAAC72100.032.101732
AACGCAG72100.031.604646
TACATGG107150.029.2083932
GTACATG108600.029.042481
ACATGGG113200.027.1217293
ACGCAGA83600.026.8301097
CATGGGG76200.026.6268754
GGTATCA41300.025.6482431
CGCAGAG87400.025.5274358
GCAGAGT97000.021.2222619
AGAGTAC82450.018.6898810-11
ATGGGGG47200.018.6563685
CAGAGTA90250.016.38236410-11
GTATAAC8400.016.2943211
GTATAGG9300.015.997231
ATGGGGA47450.015.9248345
Produced by FastQC (version 0.11.5)