FastQCFastQC Report
Mon 15 Aug 2016
SRR2920975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3246980
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70850.041.4594541
GGTATCA44650.034.4282341
ATCAACG87750.033.3306433
TCAACGC88650.032.858384
CAACGCA88450.032.8258325
TATCAAC89350.032.7938772
AACGCAG94050.030.8651076
ACGCAGA108000.026.7151837
CGCAGAG112200.025.609118
GTACATG105750.024.339961
TACATGG106300.023.5308842
ACATGGG108600.022.3789483
CATGGGG77550.021.9684934
GCAGAGT126450.021.5940489
AGAGTAC110950.020.94003710-11
CCGTATA4304.9112714E-1119.368679
GTATAGA12500.019.0663031
AGTACTT65300.018.54407512-13
GAGTACT60700.017.79270212-13
CCTATAC10100.017.6933143
Produced by FastQC (version 0.11.5)