FastQCFastQC Report
Mon 15 Aug 2016
SRR2920974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799224
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62900.037.002221
TCAACGC71350.031.3568614
ATCAACG73800.030.4787673
CAACGCA73600.030.2354875
TATCAAC76200.029.910872
AACGCAG75950.029.4561146
GTACATG113050.028.8017181
CATGGGG73750.028.4000644
TACATGG113600.028.1307432
GGTATCA42350.027.267791
ACATGGG112900.027.2495023
ACGCAGA86050.025.583877
CGCAGAG90050.024.1831448
TATACCG3603.6379788E-1223.139225
GCAGAGT102100.019.9886639
TACCGTA2751.3193512E-619.4726587
TTAGCGC2851.8563078E-618.7904134
ATGGGGA40850.018.0614575
AGAGTAC86850.017.88044510-11
GTAAGGT11100.017.6900734
Produced by FastQC (version 0.11.5)