##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920974_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2799224 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.90765690777158 31.0 28.0 33.0 16.0 34.0 2 28.03216641469207 31.0 28.0 34.0 16.0 34.0 3 28.050158186697455 31.0 28.0 34.0 16.0 34.0 4 31.432564167783642 35.0 32.0 37.0 17.0 37.0 5 31.506489298462718 35.0 32.0 37.0 17.0 37.0 6 31.40639763020037 35.0 32.0 37.0 16.0 37.0 7 31.3100973698425 35.0 32.0 37.0 15.0 37.0 8 31.28382258797438 35.0 32.0 37.0 13.0 37.0 9 32.36097254096135 37.0 32.0 39.0 11.0 39.0 10-11 32.328470318916956 37.0 32.0 39.0 11.0 39.0 12-13 32.24887915365115 37.0 32.0 39.0 10.0 39.0 14-15 33.07702420385078 38.0 32.0 40.0 10.0 41.0 16-17 32.933905253741756 38.0 32.0 40.0 10.0 41.0 18-19 33.0297544962461 38.0 32.0 40.0 10.0 41.0 20-21 33.05192635530419 38.0 32.0 40.0 10.0 41.0 22-23 32.99230179506892 38.0 32.0 40.0 10.0 41.0 24-25 32.914706897340125 38.0 31.5 40.0 9.5 41.0 26-27 32.767653821201876 38.0 31.0 40.0 8.0 41.0 28-29 32.682236219752326 38.0 31.0 40.0 8.0 41.0 30-31 32.55458244856432 38.0 31.0 40.0 7.5 41.0 32-33 32.45761968316934 38.0 31.0 40.0 2.0 41.0 34-35 32.27774590386478 38.0 30.0 40.0 2.0 41.0 36-37 32.12252306353475 38.0 30.0 40.0 2.0 41.0 38-39 31.987946659502775 38.0 30.0 40.0 2.0 41.0 40-41 31.904594809132817 38.0 30.0 40.0 2.0 41.0 42-43 31.67337965807667 37.0 30.0 40.0 2.0 41.0 44-45 31.495823485365946 37.0 30.0 40.0 2.0 41.0 46-47 31.2972564896557 37.0 30.0 40.0 2.0 41.0 48-49 31.12941926048076 37.0 29.0 40.0 2.0 41.0 50-51 30.897904740742433 36.5 29.0 40.0 2.0 41.0 52-53 30.716821519106723 36.0 28.5 40.0 2.0 41.0 54-55 30.50196018610872 36.0 28.0 40.0 2.0 41.0 56-57 30.296828513902426 36.0 28.0 39.5 2.0 41.0 58-59 30.041651007564955 35.0 28.0 39.0 2.0 41.0 60-61 29.859319582855818 35.0 28.0 39.0 2.0 41.0 62-63 29.749463065478146 35.0 28.0 39.0 2.0 41.0 64-65 29.53269513265105 35.0 28.0 39.0 2.0 40.5 66-67 29.276052220186738 35.0 27.5 38.5 2.0 40.0 68-69 28.981906414063324 34.0 27.0 38.0 2.0 40.0 70-71 28.659626382168774 34.0 26.0 37.0 2.0 40.0 72-73 28.383529685369943 34.0 26.0 37.0 2.0 39.0 74-75 28.08182482002155 34.0 26.0 36.0 2.0 39.0 76-77 27.805231199789656 34.0 26.0 36.0 2.0 39.0 78-79 27.487496177512053 34.0 26.0 35.5 2.0 37.5 80-81 27.172495127220973 33.0 25.0 35.0 2.0 37.0 82-83 26.90600019862648 33.0 25.0 35.0 2.0 37.0 84-85 26.656831321823475 33.0 25.0 35.0 2.0 36.0 86-87 26.582095966596455 33.0 24.5 35.0 2.0 36.0 88-89 26.666610817855236 33.0 25.0 35.0 2.0 36.0 90-91 26.573379800973413 33.0 25.0 35.0 2.0 35.0 92-93 26.418025852879225 33.0 25.0 35.0 2.0 35.0 94-95 26.329866062880285 33.0 24.5 35.0 2.0 35.0 96-97 26.22808517646319 33.0 24.0 35.0 2.0 35.0 98-99 26.1619773908769 33.0 24.0 35.0 2.0 35.0 100-101 25.990871220023834 33.0 23.5 35.0 2.0 35.0 102-103 24.67649534299506 31.0 19.5 34.0 2.0 35.0 104-105 25.671354453948666 32.5 23.0 35.0 2.0 35.0 106-107 25.864119305921925 33.0 23.0 35.0 2.0 35.0 108-109 25.858161940594965 33.0 23.0 35.0 2.0 35.0 110-111 25.75436335212902 33.0 22.5 35.0 2.0 35.0 112-113 25.65996540469787 33.0 20.0 35.0 2.0 35.0 114-115 25.527981683495142 33.0 19.5 35.0 2.0 35.0 116-117 25.399063633349815 33.0 19.0 35.0 2.0 35.0 118-119 25.208187876354305 33.0 17.5 35.0 2.0 35.0 120-121 24.992016358819445 33.0 11.0 35.0 2.0 35.0 122-123 24.7637572055684 33.0 2.0 35.0 2.0 35.0 124-125 23.635991974918763 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 196861.0 3 27521.0 4 16986.0 5 14932.0 6 16133.0 7 18159.0 8 19460.0 9 20617.0 10 21464.0 11 22245.0 12 21972.0 13 22208.0 14 22493.0 15 20538.0 16 17125.0 17 15212.0 18 14417.0 19 13739.0 20 13944.0 21 14981.0 22 18222.0 23 23949.0 24 32481.0 25 41399.0 26 48502.0 27 54752.0 28 63347.0 29 73690.0 30 85039.0 31 100815.0 32 122038.0 33 150148.0 34 190715.0 35 256178.0 36 379679.0 37 464207.0 38 142830.0 39 226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.298739913230555 18.807170272530392 12.74274360128939 25.15134621294966 2 15.584469412073668 19.89466669763304 39.82331303249504 24.697550857798255 3 19.139811534069224 24.561458674278104 28.90842408121787 27.390305710434802 4 12.863794507444808 15.985823220461498 36.53529312767784 34.61508914441585 5 13.938454810433127 37.606284572185615 34.35838382423663 14.096876793144622 6 32.893055359557565 36.32205754421566 17.101210582819622 13.683676513407155 7 28.44731587538571 32.2651567360433 21.180006637921394 18.10752075064959 8 26.26994873260571 35.904396134402745 19.72658580589843 18.09906932709312 9 26.4497918278777 16.3711458505262 19.386908627907427 37.792153693688675 10-11 25.292815640603934 26.44757274148035 27.42830797404927 20.831303643866445 12-13 25.69029862744131 23.66557985227613 27.547654417318146 23.09646710296441 14-15 22.950034010397463 25.063894533471657 24.890466514048583 27.095604942082296 16-17 23.193256386219826 28.0726627207097 25.61384662211984 23.120234270950633 18-19 23.23379081155571 27.32728462992849 26.95775344760911 22.48117111090669 20-21 24.119394453623897 26.446460947617563 26.707221006564552 22.72692359219399 22-23 23.70662584533383 26.19786438219628 26.494843151054415 23.60066662141548 24-25 23.399365560461188 26.362947359094957 26.75504664161275 23.4826404388311 26-27 23.30960377338943 26.620482919825477 26.580471216402223 23.489442090382866 28-29 23.23262833942953 26.785225297898524 26.614244791141022 23.367901571530926 30-31 23.617435646629264 26.56791370738541 26.487675737141696 23.326974908843628 32-33 23.30172576399745 26.53388939220298 26.477856005807325 23.686528837992242 34-35 23.530269193270474 26.590556781956277 26.571068145206954 23.3081058795663 36-37 23.638073318945022 26.413444509903606 26.533182376329524 23.415299794821852 38-39 23.44160359619153 26.645341400447663 26.441192583480145 23.471862419880654 40-41 23.577485408320527 26.51788119311317 26.581452973315926 23.323180425250374 42-43 23.60366494746473 26.435614595342116 26.456460082588855 23.504260374604304 44-45 23.546951486902028 26.524774555519137 26.42169125560318 23.50658270197566 46-47 23.534246966911603 26.585004189422644 26.360597631412986 23.520151212252767 48-49 23.526522233612578 26.673386560177253 26.307293478041473 23.492797728168696 50-51 23.389536756295488 26.79991689798692 26.3351565638711 23.4753897818465 52-53 23.63069022972409 26.613095102001154 26.227518448233482 23.52869622004127 54-55 23.585936944599943 26.60209751212927 26.282294252579558 23.52967129069123 56-57 23.46655496888419 26.70535006180293 26.18974214245397 23.63835282685891 58-59 23.477111569043277 26.49950554758461 26.363459680692348 23.65992320267976 60-61 23.478108186026205 26.55434755038677 26.441743828676582 23.52580043491044 62-63 23.336732192964718 26.67296777609542 26.47209088460145 23.518209146338414 64-65 23.366231114055047 26.606491023671897 26.38473111387641 23.642546748396644 66-67 23.4070763897423 26.62654006967645 26.247363555042398 23.71901998553885 68-69 23.620577582312528 26.44884568742434 26.340687216240312 23.589889514022822 70-71 23.393823014540388 26.329891750919938 26.476796113036333 23.79948912150334 72-73 23.557990070793515 26.342283767510583 26.39375014849507 23.705976013200832 74-75 23.377604186668872 26.53471805308037 26.38042234297625 23.70725541727451 76-77 23.484080406060034 26.373360598344092 26.42853723503068 23.714021760565192 78-79 23.594183017218203 26.38448066289954 26.398413127954505 23.622923191927743 80-81 23.54804470830496 26.40324358852526 26.338526866638894 23.710184836530885 82-83 23.57835340013236 26.42976501258845 26.355368557182118 23.636513030097074 84-85 23.582563515033495 26.426287229616896 26.248897229368612 23.742252025981 86-87 23.412966234768486 26.379468967106547 26.436884688009687 23.77068011011528 88-89 23.454017970694736 26.285445537763323 26.404996527609082 23.855539963932863 90-91 23.42752286891963 26.38812180847909 26.415665254603617 23.768690067997657 92-93 23.46439619966038 26.340302813884755 26.411899480281086 23.783401506173785 94-95 23.34443051317773 26.368035406178397 26.49272014403409 23.794813936609778 96-97 23.48997484556693 26.125867195596886 26.527727216053393 23.85643074278279 98-99 23.401737498910403 26.195754066524817 26.402724210809104 23.99978422375568 100-101 23.323511305336446 26.317371912572874 26.499270583115475 23.859846198975205 102-103 23.212047993474823 26.21502530095064 26.61155899836567 23.961367707208865 104-105 23.185875138620542 26.280846642810594 26.478684591112277 24.054593627456587 106-107 23.103694753904303 26.36405652046449 26.492489384056423 24.039759341574786 108-109 23.075138040418256 26.342768975834684 26.49506048518556 24.08703249856149 110-111 23.076667466434323 26.42041683556771 26.422989431454525 24.079926266543445 112-113 23.14326650481873 26.465531516137432 26.43273560583746 23.958466373206385 114-115 23.099604192882904 26.52927750730839 26.352186157255257 24.018932142553446 116-117 23.03789355336399 26.509732090846782 26.446443088396144 24.005931267393088 118-119 23.113254900783932 26.376954963625714 26.306253489969787 24.203536645620563 120-121 23.042782055459334 26.54098357141632 26.375282955773155 24.04095141735119 122-123 23.125716776408634 26.480962775858437 26.33707158653657 24.056248861196362 124-125 23.127276899584796 26.50900894460521 26.214924308280054 24.14878984752994 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 427.0 1 338.0 2 222.5 3 177.5 4 147.0 5 140.0 6 160.0 7 210.0 8 254.0 9 284.0 10 330.5 11 368.5 12 418.0 13 488.0 14 555.0 15 627.5 16 750.5 17 889.5 18 1023.0 19 1261.0 20 1564.5 21 1949.5 22 2613.0 23 3533.5 24 4700.5 25 6482.0 26 9165.5 27 12418.5 28 15936.5 29 19963.5 30 24563.0 31 30092.0 32 36268.0 33 41636.5 34 46481.0 35 51344.5 36 57099.5 37 62506.5 38 66734.5 39 71078.5 40 76140.5 41 81699.5 42 86257.5 43 91698.5 44 96847.5 45 100743.0 46 104467.0 47 107505.0 48 109695.0 49 111247.0 50 112471.0 51 111966.5 52 109419.5 53 106903.5 54 104341.5 55 99081.0 56 91808.5 57 84185.0 58 77380.0 59 69754.5 60 60060.5 61 50718.0 62 42006.0 63 32424.5 64 24295.5 65 19037.0 66 14589.0 67 11100.0 68 8459.5 69 6446.0 70 5143.5 71 3896.5 72 2969.0 73 2493.0 74 1962.0 75 1448.5 76 1069.5 77 753.0 78 499.5 79 345.5 80 253.5 81 170.5 82 118.5 83 73.0 84 45.0 85 39.5 86 33.0 87 26.5 88 30.0 89 27.0 90 14.0 91 9.0 92 9.5 93 9.0 94 5.0 95 4.0 96 5.5 97 4.5 98 4.5 99 4.5 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03586708316304805 2 0.0174691271580981 3 0.022434789070113717 4 0.02011271695298411 5 0.013217948974430058 6 0.005680145640363186 7 0.005537248894693672 8 0.006216008436623865 9 0.0035009702689030964 10-11 0.005376490055815469 12-13 0.0077878726389885205 14-15 0.0028579349133902826 16-17 0.002572141422051254 18-19 5.3586279626067796E-5 20-21 0.003536694455320475 22-23 0.0012146223381908701 24-25 0.002161313278251401 26-27 0.0015361400159472768 28-29 0.0026614518880947007 30-31 0.0018040714140776157 32-33 0.0 34-35 0.005608697267528429 36-37 0.006894767978554057 38-39 0.0014468295499038305 40-41 9.288288468518418E-4 42-43 0.0020362786257905763 44-45 0.0011253118721474237 46-47 0.018183610886445673 48-49 0.0025900035152599437 50-51 0.009091805443222837 52-53 0.0014289674566951413 54-55 1.0717255925213559E-4 56-57 1.428967456695141E-4 58-59 0.006001663318119593 60-61 9.645530332692203E-4 62-63 0.00878814985867512 64-65 0.008966770790762012 66-67 0.0 68-69 0.003233038870772757 70-71 0.004429799115754938 72-73 0.011056635696178655 74-75 0.0019112439733297514 76-77 0.001143173965356113 78-79 2.3220721171296046E-4 80-81 0.0036438670145726103 82-83 0.0011253118721474237 84-85 8.752425672257741E-4 86-87 0.012110499195491321 88-89 0.0 90-91 3.572418641737853E-4 92-93 0.00753780333406687 94-95 0.005876628665658768 96-97 0.004108281437998532 98-99 0.001000277219686599 100-101 0.014754088990377332 102-103 0.007805734732197209 104-105 0.007716424266153763 106-107 0.017344092505637277 108-109 0.011270980814682927 110-111 0.017665610183393684 112-113 0.003375935616442271 114-115 0.013789535957108114 116-117 0.005698007733571876 118-119 0.0046798684206765875 120-121 0.007144837283475706 122-123 0.008359459621666575 124-125 0.05572973081111051 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2799224.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.19549073317661 #Duplication Level Percentage of deduplicated Percentage of total 1 75.98365609392698 44.97889810180934 2 12.838144613608224 15.199205410120536 3 4.7456489036313565 8.427630470934592 4 2.2798062572361064 5.398170006946319 5 1.2514343217641888 3.7039634398585606 6 0.7401872459563892 2.6289448355296177 7 0.4727227523545863 1.958813871445736 8 0.3270561319370282 1.5488198581845554 9 0.22567204392289122 1.202289064629708 >10 1.0681011945385204 10.67419706992259 >50 0.047792428630318975 1.9331778369543104 >100 0.01880335959652174 1.8981763950855057 >500 7.91905478720477E-4 0.3049183737192678 >1k 1.827474181126885E-4 0.14279526485930116 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.572418641737853E-5 2 0.0 0.0 0.0 0.0 3.572418641737853E-5 3 0.0 0.0 0.0 0.0 3.572418641737853E-5 4 0.0 0.0 0.0 0.0 3.572418641737853E-5 5 0.0 0.0 0.0 0.0 3.572418641737853E-5 6 0.0 0.0 0.0 0.0 3.572418641737853E-5 7 0.0 0.0 0.0 0.0 3.572418641737853E-5 8 0.0 0.0 0.0 0.0 3.572418641737853E-5 9 0.0 0.0 0.0 0.0 3.572418641737853E-5 10-11 0.0 0.0 0.0 0.0 3.572418641737853E-5 12-13 0.0 0.0 0.0 0.0 2.3220721171296043E-4 14-15 0.0 0.0 0.0 0.0 3.39379770965096E-4 16-17 1.7862093208689264E-5 0.0 0.0 0.0 3.572418641737853E-4 18-19 7.144837283475706E-5 0.0 0.0 0.0 3.929660505911638E-4 20-21 7.144837283475706E-5 0.0 0.0 7.144837283475706E-5 4.644144234259209E-4 22-23 7.144837283475706E-5 0.0 0.0 2.67931398130339E-4 5.001386098432995E-4 24-25 1.428967456695141E-4 0.0 0.0 5.537248894693673E-4 5.35862796260678E-4 26-27 1.428967456695141E-4 0.0 0.0 0.0010360014061039774 5.894490758867458E-4 28-29 1.428967456695141E-4 0.0 0.0 0.002715038167720768 6.073111690954351E-4 30-31 1.428967456695141E-4 0.0 0.0 0.010002772196865989 6.251732623041243E-4 32-33 1.428967456695141E-4 0.0 0.0 0.025274861890295312 6.78759541930192E-4 34-35 1.428967456695141E-4 0.0 0.0 0.0457626828006619 7.144837283475706E-4 36-37 1.428967456695141E-4 0.0 0.0 0.0837017687759179 7.144837283475706E-4 38-39 2.1434511850427118E-4 0.0 0.0 0.146754957802591 7.144837283475706E-4 40-41 2.1434511850427118E-4 0.0 0.0 0.21700657039236587 7.144837283475706E-4 42-43 2.3220721171296046E-4 0.0 0.0 0.28441811016195917 7.144837283475706E-4 44-45 2.67931398130339E-4 0.0 0.0 0.3597604193162105 7.323458215562599E-4 46-47 2.857934913390282E-4 0.0 0.0 0.4493030925713698 7.859321011823277E-4 48-49 2.857934913390282E-4 0.0 0.0 0.5452403951952398 8.573804740170847E-4 50-51 2.857934913390282E-4 0.0 0.0 0.6463398427564211 8.931046604344632E-4 52-53 2.857934913390282E-4 0.0 0.0 0.7503508115106187 9.288288468518418E-4 54-55 2.857934913390282E-4 0.0 0.0 0.862596205234022 9.288288468518418E-4 56-57 2.857934913390282E-4 0.0 0.0 0.9885775486349073 9.466909400605311E-4 58-59 2.857934913390282E-4 0.0 0.0 1.1285270489249877 9.645530332692203E-4 60-61 2.857934913390282E-4 0.0 0.0 1.278086355361343 9.645530332692203E-4 62-63 2.857934913390282E-4 1.7862093208689264E-5 0.0 1.4288960083223063 0.0011074497789387344 64-65 2.857934913390282E-4 3.572418641737853E-5 0.0 1.5887974667264928 0.0011074497789387344 66-67 2.857934913390282E-4 3.572418641737853E-5 0.0 1.768418676033072 0.0011431739653561131 68-69 2.857934913390282E-4 3.572418641737853E-5 0.0 1.9696530181221652 0.0011788981517734916 70-71 3.0365558454771753E-4 3.572418641737853E-5 0.0 2.194501047433146 0.0012146223381908701 72-73 3.215176777564068E-4 3.572418641737853E-5 0.0 2.4221534253778905 0.0013039328042343163 74-75 3.215176777564068E-4 3.572418641737853E-5 0.0 2.6475373174851313 0.0013753811770690736 76-77 3.215176777564068E-4 3.572418641737853E-5 0.0 2.892676684681183 0.0013932432702777628 78-79 3.215176777564068E-4 3.572418641737853E-5 0.0 3.1702357510510053 0.0013932432702777628 80-81 3.572418641737853E-4 3.572418641737853E-5 0.0 3.470515399982281 0.0013932432702777628 82-83 3.572418641737853E-4 3.572418641737853E-5 0.0 3.7841201704472383 0.0015182779227385875 84-85 3.572418641737853E-4 3.572418641737853E-5 0.0 4.099278943021352 0.0015718642023646553 86-87 3.7510395738247457E-4 3.572418641737853E-5 0.0 4.435836503259475 0.0016433125751994125 88-89 3.929660505911638E-4 3.572418641737853E-5 0.0 4.812583773217149 0.0017147609480341695 90-91 4.108281437998531E-4 3.572418641737853E-5 0.0 5.215606182284805 0.0017147609480341695 92-93 4.644144234259209E-4 3.572418641737853E-5 0.0 5.630935573573247 0.0017862093208689265 94-95 4.644144234259209E-4 3.572418641737853E-5 0.0 6.055892633101173 0.0017862093208689265 96-97 4.644144234259209E-4 3.572418641737853E-5 0.0 6.4954966090602255 0.0019112439733297514 98-99 4.644144234259209E-4 5.3586279626067796E-5 0.0 6.9663949723209 0.002000554439373198 100-101 4.822765166346102E-4 7.144837283475706E-5 0.0 7.484252778627219 0.002000554439373198 102-103 5.001386098432995E-4 7.144837283475706E-5 0.0 8.018650883244785 0.002018416532581887 104-105 6.430353555128135E-4 7.144837283475706E-5 0.0 8.559300720485393 0.0020362786257905763 106-107 6.608974487215028E-4 7.144837283475706E-5 0.0 9.112132505294325 0.0020362786257905763 108-109 6.78759541930192E-4 7.144837283475706E-5 0.0 9.680879415152198 0.002072002812207955 110-111 6.78759541930192E-4 7.144837283475706E-5 0.0 10.292548934990554 0.002143451185042712 112-113 6.78759541930192E-4 7.144837283475706E-5 0.0 10.934548289097265 0.0021791753714600903 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6290 0.0 37.00222 1 TCAACGC 7135 0.0 31.356861 4 ATCAACG 7380 0.0 30.478767 3 CAACGCA 7360 0.0 30.235487 5 TATCAAC 7620 0.0 29.91087 2 AACGCAG 7595 0.0 29.456114 6 GTACATG 11305 0.0 28.801718 1 CATGGGG 7375 0.0 28.400064 4 TACATGG 11360 0.0 28.130743 2 GGTATCA 4235 0.0 27.26779 1 ACATGGG 11290 0.0 27.249502 3 ACGCAGA 8605 0.0 25.58387 7 CGCAGAG 9005 0.0 24.183144 8 TATACCG 360 3.6379788E-12 23.13922 5 GCAGAGT 10210 0.0 19.988663 9 TACCGTA 275 1.3193512E-6 19.472658 7 TTAGCGC 285 1.8563078E-6 18.790413 4 ATGGGGA 4085 0.0 18.061457 5 AGAGTAC 8685 0.0 17.880445 10-11 GTAAGGT 1110 0.0 17.690073 4 >>END_MODULE