FastQCFastQC Report
Mon 15 Aug 2016
SRR2920974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799224
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31210.11149518580863839No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80550.041.517731
GGTATCA52950.037.489921
TCAACGC100000.032.8936584
CAACGCA100750.032.418555
TATCAAC104800.031.838542
ATCAACG103600.031.8081473
AACGCAG107550.030.3650426
GTACATG115900.026.8487051
ACGCAGA121650.026.6513777
CATGGGG72750.026.375214
CGCAGAG124350.025.978418
TACATGG117450.025.974272
ACATGGG116450.025.5860673
GCAGAGT136900.022.5543449
AGAGTAC120750.021.60549710-11
GAGTACT65550.021.01277412-13
AGTACTT73450.020.53483812-13
GTATAGC12300.019.3861311
GTACTTT73200.018.85740914-15
CAGAGTA129900.018.77831310-11
Produced by FastQC (version 0.11.5)