FastQCFastQC Report
Mon 15 Aug 2016
SRR2920973_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920973_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165677
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54350.033.073141
CAACGCA57500.029.3899045
TCAACGC57750.029.3684224
AACGCAG59400.028.5480186
ATCAACG60500.028.3285833
GTACATG91550.027.6311281
TATCAAC62500.027.4220712
TATACCG1755.700804E-727.2019235
TACATGG92400.026.7922462
CATGGGG55500.026.3772724
ACATGGG91300.026.2025183
ACGCAGA69900.024.0894477
CGCAGAG70650.023.7489538
GGTATCA38850.021.6021061
TAAACGT1954.0360217E-521.3624574
GCAGAGT80300.019.561229
TAACACG2251.2127112E-418.514134
ATGGGGC24900.018.4008985
ATACCGA1959.912102E-418.3077166
ATACCGT2301.436908E-418.108726
Produced by FastQC (version 0.11.5)