FastQCFastQC Report
Mon 15 Aug 2016
SRR2920973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165677
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28680.13242972059083605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68750.035.7124821
GGTATCA48150.031.9314731
TCAACGC82150.029.4390074
ATCAACG83550.029.0883053
CAACGCA83300.028.928975
TATCAAC86000.027.9100822
AACGCAG85900.027.8416946
ACGCAGA99950.023.8111327
GTACATG91500.023.5115721
CGCAGAG100550.023.4323588
TACATGG91000.022.514982
CATGGGG52050.021.7439084
ACATGGG91500.021.678433
GCAGAGT110750.020.4684129
GAGTACT51450.019.66104112-13
AGTACTT55000.019.31161312-13
AGAGTAC101550.019.21727610-11
GTACTTT55650.018.07026514-15
GTATAGA10050.017.7889691
TACACTG19450.017.7431765
Produced by FastQC (version 0.11.5)