FastQCFastQC Report
Mon 15 Aug 2016
SRR2920972_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920972_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3076900
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64900.039.700871
TCAACGC72850.034.2198224
CAACGCA74850.032.9869775
AACGCAG76450.032.139916
ATCAACG79050.031.4616723
TATCAAC80050.031.4418182
GTACATG132050.029.831591
CATGGGG92600.028.6561034
ACATGGG131050.028.5575543
GGTATCA43200.027.9620571
TACATGG139800.027.7503852
ACGCAGA88950.027.422697
CGCAGAG94400.025.6504258
GCAGAGT103450.022.256289
ATGGGGG48100.020.6565445
AGAGTAC91950.019.73439210-11
CCTATAC8750.019.0395813
ATGGGGA49550.018.1309075
CAGAGTA99650.017.10499610-11
GTATCGC3154.8322E-616.9982456
Produced by FastQC (version 0.11.5)