FastQCFastQC Report
Mon 15 Aug 2016
SRR2920972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3076900
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35560.11557086678150087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74600.043.9193951
GGTATCA47650.040.6305731
TCAACGC95150.034.3578834
ATCAACG98600.033.3358043
TATCAAC98750.033.279212
CAACGCA98000.033.2697875
AACGCAG100300.032.5619546
GTACATG123750.028.4013731
CATGGGG86850.027.4254064
ACGCAGA119050.027.3349427
ACATGGG121200.027.3162383
TACATGG129150.026.8747962
CGCAGAG123500.026.109998
GCAGAGT135550.023.219069
AGAGTAC125300.021.74823810-11
GAGTACT67900.019.01712-13
AGTACTT75200.018.83263812-13
CAGAGTA133150.018.6786210-11
ATGGGGA48550.018.6272985
ATGGGGG43500.018.6013815
Produced by FastQC (version 0.11.5)