##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920972_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3076900
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.195926744450585	31.0	30.0	34.0	26.0	34.0
2	30.265380740355553	31.0	30.0	34.0	26.0	34.0
3	30.39175891319185	31.0	30.0	34.0	26.0	34.0
4	33.974537359030194	35.0	35.0	37.0	30.0	37.0
5	33.54766875751568	35.0	35.0	37.0	28.0	37.0
6	33.36150281127109	35.0	33.0	37.0	27.0	37.0
7	33.21143553576652	35.0	33.0	37.0	26.0	37.0
8	33.14896486723651	35.0	33.0	37.0	26.0	37.0
9	34.495505216289125	38.0	34.0	39.0	27.0	39.0
10-11	34.4131185608892	38.0	34.0	39.0	27.0	39.0
12-13	34.31642074815561	38.0	34.0	39.0	26.5	39.0
14-15	35.24542071565537	39.0	34.0	41.0	26.0	41.0
16-17	35.2469828398713	39.0	34.0	41.0	26.0	41.0
18-19	35.285951444635835	39.0	34.0	41.0	26.0	41.0
20-21	35.25269882024115	39.0	34.0	41.0	25.5	41.0
22-23	35.2158480938607	39.0	34.0	41.0	25.0	41.0
24-25	35.188368975267316	39.0	34.0	41.0	25.0	41.0
26-27	35.05047076603074	39.0	34.0	41.0	25.0	41.0
28-29	34.93252478143586	39.0	34.0	41.0	24.5	41.0
30-31	34.768517988884916	38.5	34.0	41.0	24.0	41.0
32-33	34.63621843413826	38.0	34.0	41.0	24.0	41.0
34-35	34.52043290324677	38.0	33.0	41.0	24.0	41.0
36-37	34.37710796580974	38.0	33.0	40.5	23.0	41.0
38-39	34.233677890084174	38.0	33.0	40.0	22.0	41.0
40-41	34.04070395527967	38.0	33.0	40.0	20.5	41.0
42-43	33.89354025155188	38.0	33.0	40.0	19.5	41.0
44-45	33.66928174461309	38.0	33.0	40.0	18.0	41.0
46-47	33.51781224609185	38.0	32.5	40.0	16.5	41.0
48-49	33.3107042802821	38.0	32.0	40.0	15.0	41.0
50-51	33.102195391465436	38.0	32.0	40.0	13.5	41.0
52-53	32.849521596411975	37.5	32.0	40.0	11.0	41.0
54-55	32.650687867659	37.0	31.0	40.0	9.0	41.0
56-57	32.503994929961976	37.0	31.0	40.0	8.0	41.0
58-59	32.42125223439176	37.0	31.0	40.0	8.0	41.0
60-61	32.14407244304333	36.0	31.0	40.0	7.0	41.0
62-63	31.87388507913809	36.0	31.0	39.0	6.0	41.0
64-65	31.58817966134746	35.0	31.0	39.0	2.0	41.0
66-67	31.260575579316843	35.0	30.0	39.0	2.0	40.5
68-69	30.936649387370405	35.0	30.0	38.0	2.0	40.0
70-71	30.58281175858819	35.0	30.0	37.0	2.0	40.0
72-73	30.301067795508466	35.0	30.0	37.0	2.0	39.0
74-75	30.040508953817152	35.0	29.5	36.0	2.0	39.0
76-77	29.721138808541063	34.0	29.0	36.0	2.0	39.0
78-79	29.41092755695668	34.0	29.0	35.5	2.0	37.5
80-81	29.162157041177807	34.0	29.0	35.0	2.0	37.0
82-83	28.918052260391953	34.0	29.0	35.0	2.0	36.5
84-85	28.735123500926257	34.0	29.0	35.0	2.0	36.0
86-87	28.517788358412687	34.0	29.0	35.0	2.0	36.0
88-89	28.397773408300562	34.0	29.0	35.0	2.0	35.5
90-91	28.216466248496864	34.0	28.0	35.0	2.0	35.0
92-93	28.37106730800481	34.0	29.0	35.0	2.0	35.0
94-95	28.322954434658257	34.0	29.0	35.0	2.0	35.0
96-97	28.260338652539893	34.0	29.0	35.0	2.0	35.0
98-99	28.21498667490006	34.0	29.0	35.0	2.0	35.0
100-101	28.08560092950697	34.0	29.0	35.0	2.0	35.0
102-103	26.76319786148396	32.0	25.5	34.0	2.0	35.0
104-105	27.84715834118756	34.0	27.5	35.0	2.0	35.0
106-107	28.047276967077252	34.0	29.0	35.0	2.0	35.0
108-109	28.023872891546688	34.0	29.0	35.0	2.0	35.0
110-111	27.961659949949627	34.0	29.0	35.0	2.0	35.0
112-113	27.856119308394813	34.0	29.0	35.0	2.0	35.0
114-115	27.76206636549774	34.0	29.0	35.0	2.0	35.0
116-117	27.632208716565373	34.0	27.0	35.0	2.0	35.0
118-119	27.500212876596574	34.0	27.0	35.0	2.0	35.0
120-121	27.354630147226104	34.0	27.0	35.0	2.0	35.0
122-123	27.122725795443465	34.0	27.0	35.0	2.0	35.0
124-125	25.98937502031265	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83197.0
3	27684.0
4	14334.0
5	11438.0
6	12693.0
7	15410.0
8	17441.0
9	18972.0
10	20123.0
11	21486.0
12	22245.0
13	23587.0
14	25131.0
15	21494.0
16	16974.0
17	15161.0
18	14156.0
19	14011.0
20	13713.0
21	14498.0
22	16407.0
23	21151.0
24	28595.0
25	37668.0
26	46250.0
27	54993.0
28	65113.0
29	77242.0
30	91014.0
31	109104.0
32	132899.0
33	164769.0
34	210793.0
35	291629.0
36	478087.0
37	635762.0
38	191338.0
39	338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82728248537784	19.48519477293102	13.15608661141567	25.531436130275466
2	16.032775877775567	20.696207900361955	37.29088104021682	25.980135181645664
3	18.912378262515826	24.410061736119122	29.489876674017367	27.18768332734769
4	13.546576730167398	16.16022607349276	35.13764963880373	35.15554755753612
5	13.940097633407294	37.50106455389828	34.05064221042346	14.508195602270963
6	33.76471720029823	35.67645007445946	16.405782292363405	14.153050432878905
7	28.774991370798258	31.811332823273297	21.07065804894886	18.343017756979577
8	27.114320000832087	34.92260267204752	19.382778086922155	18.580299240198244
9	26.5838964835242	15.102784391388191	19.031008911144717	39.28231021394289
10-11	25.800069856430248	25.568282385111118	27.237945336774473	21.393702421684164
12-13	26.11621578440868	22.777161872438192	27.714830103040306	23.391792240112824
14-15	23.280944462595514	24.18125931597007	24.84612496281739	27.691671258617017
16-17	23.490871483222026	27.37088735565216	25.610175781799278	23.528065379326534
18-19	23.58783123195316	26.301981645016692	27.282887637420966	22.827299485609185
20-21	24.303987985332746	25.43380880555483	27.048393493355526	23.2138097157569
22-23	23.991896388987634	25.16589273085482	27.05210831883067	23.790102561326876
24-25	23.690017371946904	25.582207277493445	27.035206148837016	23.692569201722634
26-27	23.386547695690414	25.79184814329438	27.075399952897477	23.746204208117724
28-29	23.479711526943454	25.783656910088904	27.1705003451424	23.56613121782524
30-31	23.678146010544403	25.654896698258266	26.983317371739986	23.683639919457345
32-33	23.52647453322783	25.764029171756942	26.825266585308928	23.8842297097063
34-35	23.619790760796242	25.81492983909953	26.99389564567762	23.57138375442661
36-37	23.870747299676715	25.424847395647753	26.930734399504804	23.773670905170732
38-39	23.685445070705512	25.598721946262053	26.936302454512084	23.779530528520347
40-41	23.712984376821144	25.628668400715053	26.937137467685723	23.721209754778076
42-43	23.82918945911274	25.495668649579674	26.763847609808494	23.91129428149909
44-45	23.69065385179927	25.585299530024365	26.751095290474463	23.972951327701907
46-47	23.82253446346659	25.711544860989484	26.637647652091758	23.82827302345218
48-49	23.776046445888525	25.906382262107623	26.356409709149137	23.96116158285471
50-51	23.759336609456415	26.09140441915781	26.33127852344957	23.8179804479362
52-53	23.974304832887785	25.828295113080777	26.35046897142283	23.84693108260861
54-55	23.687550137479306	25.920965045388893	26.309483268000523	24.082001549131277
56-57	23.692248276597436	26.05723235795085	26.172507838228775	24.07801152722294
58-59	23.55048558665796	25.836279102730593	26.390176401562865	24.223058909048586
60-61	23.52410724141446	26.11595737641969	26.400290152738236	23.95964522942761
62-63	23.444148547013548	26.075538387001323	26.440077518623657	24.040235547361473
64-65	23.36283185840708	25.97925105122534	26.396294679617082	24.2616224107505
66-67	23.401600009363623	26.10183527043318	26.28127223048042	24.215292489722774
68-69	23.650060297493646	25.876150459761277	26.37515915563678	24.0986300871083
70-71	23.44468463559455	25.803394964092163	26.418212420804927	24.33370797950836
72-73	23.51376729039541	25.874631302963625	26.23448481254324	24.377116594097725
74-75	23.477251996143274	26.01165990828891	26.140911038423603	24.37017705714421
76-77	23.426289446074755	25.76795456189268	26.302156262164683	24.503599729867883
78-79	23.445974710753983	25.895379719870615	26.272528857955933	24.386116711419465
80-81	23.534650357760338	26.019914818889774	26.04543371807273	24.400001105277163
82-83	23.435820781486097	25.800826954792218	26.267039584750673	24.496312678971012
84-85	23.277746778670874	25.971396700564505	26.108511538467166	24.64234498229746
86-87	23.36334369079443	25.891544946856715	26.209063577688678	24.536047784660177
88-89	23.217426769268233	25.81236488761136	26.12292323755089	24.84728510556952
90-91	23.124163140688598	25.894347289156972	26.176463464451654	24.80502610570278
92-93	23.146253480633206	25.822068226343603	26.273561068726487	24.75811722429671
94-95	23.094304367782613	25.58319344449024	26.399755286628015	24.92274690109913
96-97	23.187417668995433	25.423554163947692	26.454133148483887	24.93489501857298
98-99	23.06810215510186	25.56502567888496	26.227912639940858	25.138959526072313
100-101	22.90310652390337	25.552278591600803	26.451362278794775	25.09325260570105
102-103	22.785919334948048	25.390995485991063	26.44998578552874	25.373099393532144
104-105	22.769096585887887	25.593138324959526	26.231271893174558	25.406493195978037
106-107	22.67325041455866	25.56085077529742	26.27514473188298	25.49075407826094
108-109	22.599453872617197	25.602179308627633	26.32599881321881	25.47236800553636
110-111	22.624268102873238	25.68758996337101	26.26068995647951	25.427451977276245
112-113	22.535172582815875	25.758436877388768	26.316065760910462	25.390324778884892
114-115	22.45834463553862	25.90777498001358	26.12554118534441	25.508339199103386
116-117	22.311309286218567	25.83163877581004	26.233213154100287	25.623838783871104
118-119	22.411789990988655	25.77941626966102	26.06025493409693	25.748538805253396
120-121	22.242039322037424	25.944064967296637	26.148712861761837	25.6651828489041
122-123	22.2182949703116	25.87876459597028	26.061926208182484	25.84101422553563
124-125	22.06980670209123	25.985701606983792	26.068793983266396	25.875697707658578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	56.0
2	161.5
3	297.5
4	266.5
5	217.5
6	319.5
7	617.0
8	882.0
9	917.5
10	776.0
11	647.0
12	684.0
13	777.5
14	859.5
15	991.5
16	1135.0
17	1285.5
18	1517.0
19	1734.0
20	1951.5
21	2224.5
22	2641.0
23	3298.0
24	4062.0
25	5290.0
26	7078.5
27	9450.0
28	12357.5
29	16395.5
30	21549.5
31	27368.0
32	34337.5
33	39018.0
34	42384.5
35	47177.5
36	53502.0
37	60118.0
38	66177.0
39	71360.0
40	75932.5
41	81501.0
42	87262.5
43	94658.5
44	101814.5
45	108572.5
46	115969.5
47	121218.5
48	125579.5
49	129193.0
50	131737.0
51	133460.0
52	132323.0
53	131454.5
54	130893.5
55	124692.5
56	114039.0
57	102667.0
58	93028.5
59	83586.0
60	71898.0
61	60662.5
62	49742.5
63	37367.5
64	26678.5
65	19330.5
66	13969.0
67	10348.0
68	7300.0
69	4974.0
70	3705.0
71	2716.5
72	1904.0
73	1477.5
74	1122.0
75	790.0
76	562.0
77	352.0
78	201.5
79	104.0
80	59.5
81	33.5
82	19.5
83	15.5
84	12.0
85	6.5
86	3.5
87	4.0
88	4.0
89	3.0
90	3.5
91	5.0
92	4.0
93	2.5
94	4.0
95	7.0
96	6.0
97	6.5
98	4.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05203289024667686
2	9.750073125548441E-5
3	0.003087523156423673
4	0.12730345477591082
5	0.016055120413403103
6	0.001885014137606032
7	0.0044850336377522835
8	0.010010075075563066
9	0.02444018330137476
10-11	0.019191393935454513
12-13	0.018833891254184405
14-15	0.02811271084533134
16-17	0.0366115245864344
18-19	0.03974779810848581
20-21	0.021450160876206572
22-23	0.024066430498228736
24-25	0.021970164776235823
26-27	0.02052390392927947
28-29	0.020588904416783127
30-31	0.02489518671390035
32-33	0.025155188663914977
34-35	0.029217719132893498
36-37	0.031590236926776954
38-39	0.03162273717052878
40-41	0.034239006792550944
42-43	0.031005232539244042
44-45	0.028908966817251126
46-47	0.03950404628034711
48-49	0.03173648802366018
50-51	0.022847671357535182
52-53	0.02967272254541909
54-55	0.031931489486171145
56-57	0.025691442685820142
58-59	0.02855146413598102
60-61	0.041551561636712274
62-63	0.04907536806526049
64-65	0.042981572361792716
66-67	0.03796028470213527
68-69	0.02929896974227307
70-71	0.036042770320777406
72-73	0.03913029347720108
74-75	0.023335175013812604
76-77	0.02039390295427216
78-79	0.03017647632357243
80-81	0.02432643244824336
82-83	0.03761903214274107
84-85	0.02101140758555689
86-87	0.033068998017485134
88-89	0.03212649094868212
90-91	0.03378400338002535
92-93	0.02507393805453541
94-95	0.021060157951184634
96-97	0.016981377360330202
98-99	0.02387142903571777
100-101	0.023156423673177547
102-103	0.026308947317104876
104-105	0.024098930741980565
106-107	0.014153856153921153
108-109	0.016623874679060093
110-111	0.021060157951184634
112-113	0.012723845428840716
114-115	0.014153856153921153
116-117	0.012480093600702005
118-119	0.007231304234781761
120-121	0.004940037050277877
122-123	0.0035425265689492673
124-125	0.06529298969742273
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3076900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.50547496604005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.49467215143707	32.783882109778574
2	13.968138798385318	12.991898584209624
3	5.647620717621089	7.879358517030501
4	2.9312345620697475	5.45273822183704
5	1.697049263457125	3.9461041018921015
6	1.1113299423711094	3.1009756088370217
7	0.7790425627428538	2.5360821079382228
8	0.552470318159043	2.0554315640500445
9	0.40008008887639596	1.6745323101887077
>10	2.2431630040026427	18.597842641908805
>50	0.1239662233089765	3.9349576882474717
>100	0.04827007119565916	3.749551509522093
>500	0.0021159259808094053	0.6630664383942978
>1k	8.463703921073032E-4	0.6335785961655729
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3556	0.11557086678150087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.250024375182814E-5
2	0.0	0.0	0.0	0.0	3.250024375182814E-5
3	0.0	0.0	0.0	0.0	3.250024375182814E-5
4	0.0	0.0	0.0	0.0	3.250024375182814E-5
5	0.0	0.0	0.0	0.0	3.250024375182814E-5
6	0.0	0.0	0.0	0.0	3.250024375182814E-5
7	0.0	0.0	0.0	3.250024375182814E-5	3.250024375182814E-5
8	0.0	0.0	0.0	3.250024375182814E-5	3.250024375182814E-5
9	0.0	0.0	0.0	3.250024375182814E-5	3.250024375182814E-5
10-11	0.0	0.0	0.0	3.250024375182814E-5	3.250024375182814E-5
12-13	0.0	0.0	0.0	6.500048750365628E-5	1.3000097500731256E-4
14-15	0.0	0.0	0.0	6.500048750365628E-5	1.625012187591407E-4
16-17	0.0	0.0	0.0	9.750073125548441E-5	1.9500146251096883E-4
18-19	0.0	0.0	0.0	9.750073125548441E-5	1.9500146251096883E-4
20-21	0.0	0.0	0.0	1.625012187591407E-4	1.9500146251096883E-4
22-23	0.0	0.0	0.0	3.737528031460236E-4	2.4375182813871104E-4
24-25	0.0	0.0	0.0	7.962559719197895E-4	3.250024375182814E-4
26-27	0.0	0.0	0.0	0.0014787610907081804	3.250024375182814E-4
28-29	0.0	0.0	0.0	0.004387532906496799	3.250024375182814E-4
30-31	0.0	0.0	0.0	0.015746368097760734	3.250024375182814E-4
32-33	0.0	0.0	0.0	0.04020280152101141	3.250024375182814E-4
34-35	0.0	0.0	0.0	0.07161428710715331	3.250024375182814E-4
36-37	0.0	0.0	0.0	0.1262309467321005	3.250024375182814E-4
38-39	0.0	0.0	0.0	0.2148103610777081	3.250024375182814E-4
40-41	0.0	0.0	0.0	0.3102473268549514	3.250024375182814E-4
42-43	0.0	0.0	0.0	0.40524553934154506	3.250024375182814E-4
44-45	0.0	0.0	0.0	0.512853846403848	3.250024375182814E-4
46-47	0.0	0.0	0.0	0.640498553739153	3.737528031460236E-4
48-49	0.0	0.0	0.0	0.7719782898371738	3.9000292502193765E-4
50-51	0.0	0.0	0.0	0.908674315057363	3.9000292502193765E-4
52-53	0.0	0.0	0.0	1.0435828268712015	4.3875329064967984E-4
54-55	0.0	0.0	0.0	1.1914426858201437	4.550034125255939E-4
56-57	0.0	0.0	0.0	1.3571939289544672	4.71253534401508E-4
58-59	0.0	0.0	0.0	1.5410640579804349	4.875036562774221E-4
60-61	0.0	0.0	0.0	1.7349117618382137	4.875036562774221E-4
62-63	0.0	0.0	0.0	1.9315707367805257	5.200039000292502E-4
64-65	0.0	0.0	0.0	2.1346810101075757	5.362540219051643E-4
66-67	0.0	0.0	0.0	2.36835776268322	5.525041437810784E-4
68-69	0.0	0.0	0.0	2.6273684552634142	5.525041437810784E-4
70-71	0.0	0.0	0.0	2.901540511553837	5.687542656569924E-4
72-73	0.0	0.0	0.0	3.1780688355162665	6.175046312847346E-4
74-75	0.0	0.0	0.0	3.4669635022262666	6.337547531606487E-4
76-77	0.0	0.0	0.0	3.7826383697877732	6.500048750365628E-4
78-79	0.0	0.0	0.0	4.130325977444831	6.500048750365628E-4
80-81	0.0	0.0	0.0	4.505135038512789	6.500048750365628E-4
82-83	0.0	0.0	0.0	4.888069160518704	7.475056062920471E-4
84-85	0.0	0.0	0.0	5.27716207871559	7.475056062920471E-4
86-87	0.0	0.0	0.0	5.693278949592122	7.475056062920471E-4
88-89	0.0	0.0	0.0	6.15164613734603	7.475056062920471E-4
90-91	0.0	0.0	0.0	6.638727290454678	7.475056062920471E-4
92-93	0.0	0.0	0.0	7.136029770223277	7.475056062920471E-4
94-95	0.0	0.0	0.0	7.640466053495401	7.800058500438753E-4
96-97	0.0	0.0	0.0	8.169960024700185	8.287562156716175E-4
98-99	0.0	0.0	0.0	8.744174331307484	8.450063375475316E-4
100-101	0.0	0.0	0.0	9.358786440898307	8.450063375475316E-4
102-103	0.0	0.0	0.0	9.992508693815203	8.612564594234457E-4
104-105	0.0	0.0	0.0	10.6319672397543	8.775065812993598E-4
106-107	0.0	0.0	0.0	11.277503331274985	8.775065812993598E-4
108-109	0.0	0.0	0.0	11.95644967337255	8.775065812993598E-4
110-111	0.0	0.0	0.0	12.674250056875426	8.775065812993598E-4
112-113	0.0	0.0	0.0	13.422909421820663	8.775065812993598E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7460	0.0	43.919395	1
GGTATCA	4765	0.0	40.630573	1
TCAACGC	9515	0.0	34.357883	4
ATCAACG	9860	0.0	33.335804	3
TATCAAC	9875	0.0	33.27921	2
CAACGCA	9800	0.0	33.269787	5
AACGCAG	10030	0.0	32.561954	6
GTACATG	12375	0.0	28.401373	1
CATGGGG	8685	0.0	27.425406	4
ACGCAGA	11905	0.0	27.334942	7
ACATGGG	12120	0.0	27.316238	3
TACATGG	12915	0.0	26.874796	2
CGCAGAG	12350	0.0	26.10999	8
GCAGAGT	13555	0.0	23.21906	9
AGAGTAC	12530	0.0	21.748238	10-11
GAGTACT	6790	0.0	19.017	12-13
AGTACTT	7520	0.0	18.832638	12-13
CAGAGTA	13315	0.0	18.67862	10-11
ATGGGGA	4855	0.0	18.627298	5
ATGGGGG	4350	0.0	18.601381	5
>>END_MODULE