FastQCFastQC Report
Mon 15 Aug 2016
SRR2920971_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920971_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630277
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44650.046.252971
GGTATCA26900.041.1521261
TCAACGC54650.036.800954
CAACGCA56200.035.573555
TATCAAC58050.035.0575182
ATCAACG58150.034.5879023
AACGCAG59700.033.585756
GTACATG89800.030.8182661
ACGCAGA69450.028.785027
TACATGG92650.028.6448612
CGCAGAG71500.027.6268628
ACATGGG95050.027.4207483
CATGGGG69150.024.8678464
GCAGAGT81550.022.398249
AGAGTAC69600.021.4158410-11
CAGAGTA76250.018.76773510-11
ATACGGT2000.00117519717.8492816
TCTATAC8200.016.6905733
TATAGGG5156.366463E-1016.176262
GTACGTA3006.028897E-515.8708571
Produced by FastQC (version 0.11.5)