FastQCFastQC Report
Mon 15 Aug 2016
SRR2920971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630277
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40840.15526881769486636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32580.12386528110917595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27180.10333512401925729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39250.054.2036131
GTATCAA70100.052.1975361
TCAACGC91700.039.0463754
TATCAAC91850.038.912562
CAACGCA93550.038.288325
ATCAACG94750.037.9781073
AACGCAG98450.036.4333886
ACGCAGA111450.031.7572657
CGCAGAG116050.030.3976868
GCAGAGT128100.026.3318549
GTACATG92000.025.3272821
TACATGG91150.024.5724742
AGAGTAC116600.023.77781910-11
GTGGTAT23350.023.4801351
ACATGGG92300.023.2368033
AGTACTT76500.022.43956812-13
GAGTACT72100.021.99338312-13
CAGAGTA123650.021.72442610-11
CATGGGG68350.020.6578644
TGGTATC25050.020.2129142
Produced by FastQC (version 0.11.5)