FastQCFastQC Report
Mon 15 Aug 2016
SRR2920970_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920970_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681248
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26610.1582752812196654No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25990.15458754449075926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA1454.05641E-628.7349991
GTATCAA71100.027.6264741
CATGGGG47250.026.9527234
GTACATG103000.024.0401551
TACATGG102000.023.5720712
TCAACGC82600.023.4870364
AACGCAG84200.023.1779446
TATCAAC84500.023.1715832
ATCAACG84100.023.068813
CAACGCA84450.022.9711515
ACATGGG106150.021.9770643
ACGCAGA92500.021.0981947
CGCAGAG93000.020.792838
GCAGAGT95200.019.312339
GGTATCA54950.019.2811721
CCGTACT2201.0204974E-418.9350324
ATGGGGA26650.018.086425
GAGTACT23000.017.84718912-13
ATAGCCC6050.017.706013
GTATAGG4059.3623385E-917.6362951
Produced by FastQC (version 0.11.5)