##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920970_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1681248
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.030238846380783	31.0	28.0	33.0	16.0	34.0
2	28.068275471554465	31.0	28.0	34.0	16.0	34.0
3	28.131871681036944	31.0	28.0	34.0	16.0	34.0
4	31.491569358000724	35.0	32.0	37.0	17.0	37.0
5	31.61531671710539	35.0	32.0	37.0	17.0	37.0
6	31.619245941110414	35.0	32.0	37.0	17.0	37.0
7	31.425116936942082	35.0	32.0	37.0	17.0	37.0
8	31.423509202687526	35.0	32.0	37.0	16.0	37.0
9	32.55036437218067	37.0	32.0	39.0	13.0	39.0
10-11	32.49690616732332	37.0	32.0	39.0	12.0	39.0
12-13	32.433504456213484	37.0	32.0	39.0	11.0	39.0
14-15	33.275632149450885	38.0	32.0	40.0	10.0	41.0
16-17	33.14496701260016	38.0	32.0	40.0	10.0	41.0
18-19	33.25731748082377	38.0	32.0	40.0	10.0	41.0
20-21	33.31022222777365	38.0	32.0	40.0	10.0	41.0
22-23	33.26726752983498	38.0	32.0	40.0	10.5	41.0
24-25	33.20920827861208	38.0	32.0	40.0	10.0	41.0
26-27	33.074825367822	38.0	32.0	40.0	10.0	41.0
28-29	32.99197738822589	38.0	31.0	40.0	9.5	41.0
30-31	32.86329024629323	38.0	31.0	40.0	8.5	41.0
32-33	32.75853086516683	38.0	31.0	40.0	8.0	41.0
34-35	32.57698358600278	38.0	31.0	40.0	7.5	41.0
36-37	32.420806151144866	38.0	30.0	40.0	2.0	41.0
38-39	32.26963333190582	38.0	30.0	40.0	2.0	41.0
40-41	32.174466824644554	38.0	30.0	40.0	2.0	41.0
42-43	31.934750851748227	38.0	30.0	40.0	2.0	41.0
44-45	31.743692780601076	37.0	30.0	40.0	2.0	41.0
46-47	31.52481222282495	37.0	30.0	40.0	2.0	41.0
48-49	31.342377210262853	37.0	30.0	40.0	2.0	41.0
50-51	31.12313650335941	37.0	29.0	40.0	2.0	41.0
52-53	30.945816738518054	36.5	29.0	40.0	2.0	41.0
54-55	30.730780646281808	36.0	29.0	40.0	2.0	41.0
56-57	30.526907987399838	36.0	28.0	40.0	2.0	41.0
58-59	30.2774602557148	35.5	28.0	39.0	2.0	41.0
60-61	30.093770223072383	35.0	28.0	39.0	2.0	41.0
62-63	29.97175461323969	35.0	28.0	39.0	2.0	41.0
64-65	29.760989752850264	35.0	28.0	39.0	2.0	40.5
66-67	29.493522668874547	35.0	28.0	38.5	2.0	40.0
68-69	29.187721561601858	34.5	28.0	38.0	2.0	40.0
70-71	28.853667037819527	34.0	27.0	37.0	2.0	40.0
72-73	28.569549227716553	34.0	26.0	37.0	2.0	39.0
74-75	28.26801370172634	34.0	26.0	36.0	2.0	39.0
76-77	27.974169783399	34.0	26.0	36.0	2.0	39.0
78-79	27.654158696396962	34.0	26.0	35.5	2.0	37.5
80-81	27.34155668884067	34.0	26.0	35.0	2.0	37.0
82-83	27.068546698642912	33.0	25.5	35.0	2.0	37.0
84-85	26.819215844420334	33.0	25.0	35.0	2.0	36.0
86-87	26.728086814080967	33.0	25.0	35.0	2.0	36.0
88-89	26.79917269790061	33.0	25.0	35.0	2.0	36.0
90-91	26.704924853442208	33.0	25.0	35.0	2.0	35.0
92-93	26.543458192961417	33.0	25.0	35.0	2.0	35.0
94-95	26.454920095072232	33.0	25.0	35.0	2.0	35.0
96-97	26.356285330897048	33.0	25.0	35.0	2.0	35.0
98-99	26.282243607129942	33.0	24.5	35.0	2.0	35.0
100-101	26.10136071537334	33.0	24.0	35.0	2.0	35.0
102-103	24.765518828869983	31.0	19.5	34.0	2.0	35.0
104-105	25.76065428776718	33.0	23.0	35.0	2.0	35.0
106-107	25.93853316108034	33.0	23.0	35.0	2.0	35.0
108-109	25.928705045299683	33.0	23.0	35.0	2.0	35.0
110-111	25.823653321818078	33.0	23.0	35.0	2.0	35.0
112-113	25.72668250014275	33.0	21.5	35.0	2.0	35.0
114-115	25.589024046422658	33.0	19.5	35.0	2.0	35.0
116-117	25.454109982584367	33.0	19.0	35.0	2.0	35.0
118-119	25.262514215630294	33.0	18.0	35.0	2.0	35.0
120-121	25.04411246883268	33.0	11.5	35.0	2.0	35.0
122-123	24.80920839757133	33.0	2.0	35.0	2.0	35.0
124-125	23.67498429737909	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109468.0
3	15406.0
4	9684.0
5	8761.0
6	10349.0
7	11393.0
8	11557.0
9	12049.0
10	12870.0
11	13646.0
12	13807.0
13	14208.0
14	14637.0
15	12950.0
16	10615.0
17	9433.0
18	8641.0
19	8211.0
20	8468.0
21	8985.0
22	11012.0
23	14361.0
24	19674.0
25	25238.0
26	28723.0
27	31911.0
28	37203.0
29	42758.0
30	50213.0
31	59314.0
32	72343.0
33	89070.0
34	114783.0
35	155126.0
36	232131.0
37	284949.0
38	87179.0
39	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.51291748281041	19.60327679232305	13.422639093503296	24.461166631363245
2	15.288451356441804	20.717252588132133	39.74075250663021	24.253543548795854
3	19.093723674127943	25.317754763049305	28.96196937750765	26.6265521853151
4	12.537397964313316	16.646132117957745	36.47798480225259	34.338485115476345
5	13.437679207524992	38.70733819865652	33.888336311697955	13.966646282120532
6	32.250111230867695	36.75127350421249	17.257379900403762	13.741235364516044
7	27.920560747663554	33.05872527932695	20.92204065706074	18.09867331594876
8	26.007359125565525	36.042227533312946	19.601017857716435	18.34939548340509
9	26.37155761121907	16.893794882135417	19.253941657461983	37.48070584918353
10-11	24.60260102713448	27.0778238168146	27.327469601744486	20.992105554306434
12-13	25.2321711170654	24.641292835849924	27.441414063004217	22.68512198408046
14-15	22.8506764453089	25.992897993415475	24.830643314507665	26.325782246767965
16-17	22.614570278554165	28.92404728241506	25.757234194039196	22.704148244991572
18-19	22.82245094120558	27.88773577723215	27.43102147928206	21.858791802280212
20-21	23.85478508985594	26.956395793220402	27.06795361145759	22.120865505466064
22-23	23.348111164742335	26.713435871081636	26.794328810168956	23.14412415400707
24-25	22.93391846188436	27.00484646323576	26.90007173336601	23.16116334151387
26-27	22.83903620506189	27.09633845240595	26.915458869672126	23.149166472860035
28-29	22.815035466983186	27.160711189520175	27.144710823117734	22.879542520378905
30-31	23.153055546550902	27.110427220079114	26.87253584355973	22.863981389810256
32-33	22.771037577751045	27.131252239780284	26.856248113380094	23.241462069088577
34-35	22.933274167678125	27.25649114552461	26.969486165913857	22.840748520883405
36-37	23.213458256574857	27.046990165596064	26.79567245778011	22.943879120048965
38-39	23.14585154916341	27.098493364976832	26.70152209989115	23.054132985968607
40-41	23.026033258903876	27.175961228478606	26.866188763729497	22.93181674888802
42-43	23.158137042229477	27.031873896262177	26.711213708624516	23.09877535288383
44-45	23.155500820676224	27.18310433011913	26.66934525234567	22.992049596858976
46-47	23.121710355084588	27.181676891615542	26.685822643614053	23.010790109685818
48-49	23.060092225379673	27.255380433408728	26.507435875702434	23.177091465509164
50-51	23.00422370623158	27.53058774087186	26.42482793326895	23.04036061962761
52-53	23.07857480196	27.30792885667959	26.291914352978125	23.321581988382288
54-55	22.994683100539305	27.24243312834611	26.441864739362487	23.3210190317521
56-57	23.154820888793193	27.262696833182055	26.212612550453652	23.3698697275711
58-59	23.050270171239315	26.983694553033743	26.555239371004234	23.410795904722704
60-61	23.108543557944667	27.18622443289541	26.58404895203682	23.1211830571231
62-63	22.825557414777983	27.264710378684548	26.63851659826936	23.271215608268115
64-65	22.769961150506543	27.238366715186395	26.608512387402794	23.383159746904266
66-67	23.073788957307002	27.34829345471146	26.352128404651904	23.22578918332964
68-69	23.35829074216444	27.039030215069914	26.389314759271294	23.213364283494354
70-71	23.001817773013922	27.00085892154192	26.61618817281692	23.38113513262724
72-73	23.043091602372296	27.16728036310207	26.49000339069759	23.299624643828036
74-75	23.19380473887869	27.1795103616688	26.40444843493165	23.222236464520865
76-77	23.13015119030731	27.042951703980222	26.63339926737966	23.19349783833281
78-79	23.165056102819133	27.10166611796566	26.609738565532	23.123539213683205
80-81	23.125695749529875	27.130179476983724	26.534887033920306	23.2092377395661
82-83	23.096636161295812	27.091886078915845	26.446913398134942	23.364564361653404
84-85	23.20273489428902	27.092226845146573	26.3292460601879	23.37579220037651
86-87	23.164410127064198	27.017768762194834	26.45627111787941	23.361549992861562
88-89	23.078124107805632	26.853979900645236	26.559050181769734	23.5088458097794
90-91	22.95158315853712	27.027851706192468	26.555939101028496	23.464626034241917
92-93	23.077357767779176	26.981531403989234	26.643394538415965	23.29771628981562
94-95	22.80484903281068	27.000553189464416	26.726992076899286	23.46760570082562
96-97	23.04618921503168	26.88637313319643	26.683896602540173	23.383541049231717
98-99	23.011639069873677	26.981193575671202	26.455270354989253	23.551896999465868
100-101	22.75759297320932	27.19371562675677	26.622000660323202	23.426690739710708
102-103	22.694102279189398	27.177471739145954	26.654874952710212	23.473551028954436
104-105	22.73963590528624	27.284499667931396	26.400837294710072	23.575027132072297
106-107	22.597238808966807	27.387427850759455	26.500146492145195	23.515186848128543
108-109	22.610270224771405	27.397001957391854	26.517231502557753	23.47549631527899
110-111	22.545971028287575	27.515720277224187	26.387132276391327	23.551176418096908
112-113	22.510456094811047	27.59281856701462	26.38364997785803	23.5130753603163
114-115	22.521425762788088	27.74665281014041	26.303466253271047	23.428455173800458
116-117	22.60408236829825	27.713333053763883	26.20080284018769	23.481781737750175
118-119	22.634138100969654	27.64663387399895	26.129117931962202	23.590110093069192
120-121	22.559305897710207	27.667698409795044	26.286310651342497	23.486685041152246
122-123	22.602305004089523	27.6209681017176	26.22597962673805	23.55074726745483
124-125	22.50524619316227	27.807561421822495	26.145354366511015	23.54183801850421
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1218.0
1	860.0
2	415.0
3	301.5
4	259.5
5	210.0
6	195.5
7	234.0
8	291.5
9	323.5
10	349.5
11	397.5
12	440.5
13	489.0
14	560.0
15	662.0
16	789.5
17	952.0
18	1133.0
19	1291.5
20	1534.5
21	1967.5
22	2537.5
23	3361.0
24	4374.5
25	5454.0
26	6978.0
27	9036.0
28	11075.0
29	13479.5
30	16390.0
31	19509.5
32	23101.5
33	26373.5
34	29286.0
35	32606.5
36	36567.5
37	40088.0
38	42754.5
39	45128.0
40	47384.5
41	50008.0
42	52289.5
43	55080.0
44	57753.5
45	60799.5
46	63678.0
47	65296.5
48	66911.5
49	68236.0
50	71506.0
51	70811.0
52	64870.0
53	61700.5
54	59853.0
55	56124.5
56	50942.0
57	45388.0
58	41230.0
59	37331.5
60	32294.5
61	27705.5
62	22968.5
63	17330.5
64	12822.0
65	9863.0
66	7320.0
67	5277.0
68	3825.0
69	2826.5
70	2155.5
71	1652.0
72	1295.5
73	1101.5
74	871.5
75	664.5
76	512.0
77	350.5
78	248.5
79	161.5
80	110.0
81	79.0
82	49.5
83	34.0
84	24.5
85	21.0
86	17.0
87	16.5
88	13.5
89	10.5
90	9.5
91	6.0
92	5.5
93	6.5
94	7.0
95	6.5
96	5.5
97	4.5
98	4.5
99	6.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0344981823026704
2	0.018200765145891622
3	0.023316012866632405
4	0.020282552008983802
5	0.014037191419707265
6	0.0035687774795865926
7	0.003806695978225699
8	0.004163573726184359
9	0.0023197053617312856
10-11	0.003925655227545252
12-13	0.006334580026266201
14-15	0.001962827613772626
16-17	0.001843868364453073
18-19	0.0
20-21	0.0033903386056072632
22-23	8.029749329069835E-4
24-25	0.0013085517425150842
26-27	0.0011301128685357543
28-29	0.002171006300081844
30-31	0.0011003730562058661
32-33	2.9739812329888273E-5
34-35	0.005501865281029331
36-37	0.006483279087915644
38-39	0.0010706332438759779
40-41	8.327147452368717E-4
42-43	0.0017249091151335199
44-45	7.434953082472068E-4
46-47	0.018468423456860616
48-49	0.0024089247987209503
50-51	0.008416366889358382
52-53	0.0013085517425150842
54-55	2.9739812329888276E-4
56-57	1.1895924931955309E-4
58-59	0.0054721254686994425
60-61	6.245360589276537E-4
62-63	0.008267667827708939
64-65	0.0086245455756676
66-67	2.9739812329888273E-5
68-69	0.0030037210453187154
70-71	0.004163573726184359
72-73	0.010587373189440226
74-75	0.0016356896781438552
76-77	0.0013382915548449724
78-79	2.6765831096899447E-4
80-81	0.0034200784179371515
82-83	8.327147452368717E-4
84-85	7.73235120577095E-4
86-87	0.01237176192923352
88-89	0.0
90-91	2.6765831096899447E-4
92-93	0.007375473457812292
94-95	0.005234206970060337
96-97	0.0038959154152153636
98-99	8.327147452368717E-4
100-101	0.015048345038923466
102-103	0.007375473457812292
104-105	0.007107815146843297
106-107	0.016089238470469554
108-109	0.010973990749728773
110-111	0.01772492814861341
112-113	0.003063200669978492
114-115	0.01255020080321285
116-117	0.005442385656369554
118-119	0.004728630160452236
120-121	0.006691457774224862
122-123	0.007315993833152515
124-125	0.058408991415900564
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1681248.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.87152268865209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60226307394285	37.14118413598863
2	13.258858625271886	13.755143720128421
3	5.6392089825653136	8.77543070055562
4	2.9904007469069898	6.204665607653924
5	1.8154743370959343	4.708570913366869
6	1.1638803188868931	3.6223346620810273
7	0.809909973071941	2.9407884480776763
8	0.5496148052254581	2.280748547141716
9	0.39085554856119104	1.8246845209659641
>10	1.70890980241224	14.446793485617885
>50	0.052330436727152804	1.8215248906959085
>100	0.016788398463579216	1.5781714726370262
>500	9.261236113974069E-4	0.35050777811680406
>1k	5.788272571233793E-4	0.549451116972547
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2661	0.1582752812196654	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2599	0.15458754449075926	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7843887397932964E-4
2	0.0	0.0	0.0	0.0	1.7843887397932964E-4
3	0.0	0.0	0.0	0.0	1.7843887397932964E-4
4	0.0	0.0	0.0	0.0	1.7843887397932964E-4
5	0.0	0.0	0.0	0.0	1.7843887397932964E-4
6	0.0	0.0	0.0	0.0	2.3791849863910618E-4
7	0.0	0.0	0.0	0.0	2.3791849863910618E-4
8	0.0	0.0	0.0	0.0	2.3791849863910618E-4
9	0.0	0.0	0.0	0.0	2.3791849863910618E-4
10-11	0.0	0.0	0.0	0.0	2.3791849863910618E-4
12-13	0.0	0.0	0.0	0.0	5.353166219379889E-4
14-15	0.0	0.0	0.0	0.0	5.353166219379889E-4
16-17	0.0	0.0	0.0	8.921943698966482E-5	5.947962465977655E-4
18-19	0.0	0.0	0.0	2.3791849863910618E-4	5.947962465977655E-4
20-21	0.0	0.0	0.0	4.7583699727821237E-4	7.434953082472069E-4
22-23	0.0	0.0	0.0	0.0013382915548449722	8.327147452368717E-4
24-25	0.0	0.0	0.0	0.002646843297360056	8.327147452368717E-4
26-27	0.0	0.0	0.0	0.004371752412493576	0.0010111536192162012
28-29	0.0	0.0	0.0	0.007553912331791621	0.0011301128685357543
30-31	0.0	0.0	0.0	0.017100392089685758	0.0011301128685357543
32-33	0.0	0.0	0.0	0.03384390643141286	0.0011301128685357543
34-35	0.0	0.0	0.0	0.05742757760901425	0.0011301128685357543
36-37	0.0	0.0	0.0	0.09540531795428159	0.0011301128685357543
38-39	0.0	0.0	0.0	0.1572343877881193	0.0011301128685357543
40-41	0.0	0.0	0.0	0.2285504577551914	0.0011301128685357543
42-43	0.0	0.0	0.0	0.2951676373741411	0.0012193323055254193
44-45	0.0	0.0	0.0	0.36773277945906846	0.001308551742515084
46-47	0.0	0.0	0.0	0.456238460952816	0.0013977711795047489
48-49	0.0	0.0	0.0	0.5449523211328727	0.001427510991834637
50-51	0.0	0.0	0.0	0.6413093130817107	0.0016059498658139667
52-53	0.0	0.0	0.0	0.7395696530196616	0.0017249091151335199
54-55	0.0	0.0	0.0	0.8469006357182284	0.001754648927463408
56-57	0.0	0.0	0.0	0.9733543177449133	0.0018141285521231848
58-59	0.0	0.0	0.0	1.1095923980281315	0.001843868364453073
60-61	0.0	0.0	0.0	1.2452356820647519	0.0019033479891128495
62-63	0.0	0.0	0.0	1.3899198690496584	0.0021412664877519558
64-65	0.0	0.0	0.0	1.5478977521460249	0.0022602257370715087
66-67	0.0	0.0	0.0	1.7258607891280762	0.002319705361731285
68-69	0.0	0.0	0.0	1.9339205161879747	0.002319705361731285
70-71	2.9739812329888273E-5	0.0	0.0	2.160151268581435	0.0024089247987209503
72-73	5.9479624659776546E-5	0.0	0.0	2.381474951940463	0.002498144235710615
74-75	5.9479624659776546E-5	0.0	0.0	2.6070811682749957	0.0025873636727002797
76-77	5.9479624659776546E-5	0.0	0.0	2.8593045166447784	0.002617103485030168
78-79	5.9479624659776546E-5	0.0	0.0	3.151081815413312	0.002617103485030168
80-81	5.9479624659776546E-5	0.0	0.0	3.460940920078418	0.002617103485030168
82-83	5.9479624659776546E-5	0.0	0.0	3.790190382382611	0.002617103485030168
84-85	5.9479624659776546E-5	0.0	0.0	4.128213089324121	0.002617103485030168
86-87	5.9479624659776546E-5	0.0	0.0	4.481759978301833	0.002825282171339386
88-89	5.9479624659776546E-5	0.0	0.0	4.883009526256686	0.003033460857648604
90-91	5.9479624659776546E-5	0.0	0.0	5.321582538685548	0.003033460857648604
92-93	5.9479624659776546E-5	0.0	0.0	5.765389758084471	0.0030929404823083805
94-95	5.9479624659776546E-5	0.0	0.0	6.213985087268505	0.0031226802946382687
96-97	5.9479624659776546E-5	0.0	0.0	6.686967062563049	0.0033605987932773746
98-99	5.9479624659776546E-5	0.0	0.0	7.202298530615352	0.0034498182302670397
100-101	5.9479624659776546E-5	0.0	0.0	7.764202544776261	0.0034498182302670397
102-103	5.9479624659776546E-5	0.0	2.9739812329888273E-5	8.331221806657911	0.0035390376672567044
104-105	5.9479624659776546E-5	0.0	5.9479624659776546E-5	8.903713194008262	0.0035687774795865926
106-107	5.9479624659776546E-5	0.0	5.9479624659776546E-5	9.490003854279678	0.0035687774795865926
108-109	5.9479624659776546E-5	0.0	5.9479624659776546E-5	10.096814985058717	0.0035687774795865926
110-111	5.9479624659776546E-5	0.0	5.9479624659776546E-5	10.745737690096881	0.0036282571042463695
112-113	5.9479624659776546E-5	0.0	5.9479624659776546E-5	11.42157492529359	0.0036282571042463695
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGA	145	4.05641E-6	28.734999	1
GTATCAA	7110	0.0	27.626474	1
CATGGGG	4725	0.0	26.952723	4
GTACATG	10300	0.0	24.040155	1
TACATGG	10200	0.0	23.572071	2
TCAACGC	8260	0.0	23.487036	4
AACGCAG	8420	0.0	23.177944	6
TATCAAC	8450	0.0	23.171583	2
ATCAACG	8410	0.0	23.06881	3
CAACGCA	8445	0.0	22.971151	5
ACATGGG	10615	0.0	21.977064	3
ACGCAGA	9250	0.0	21.098194	7
CGCAGAG	9300	0.0	20.79283	8
GCAGAGT	9520	0.0	19.31233	9
GGTATCA	5495	0.0	19.281172	1
CCGTACT	220	1.0204974E-4	18.935032	4
ATGGGGA	2665	0.0	18.08642	5
GAGTACT	2300	0.0	17.847189	12-13
ATAGCCC	605	0.0	17.70601	3
GTATAGG	405	9.3623385E-9	17.636295	1
>>END_MODULE