FastQCFastQC Report
Mon 15 Aug 2016
SRR2920970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681248
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39800.2367289061459107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31190.18551694931384305No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26920.16011914958411846No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26080.15512286111269724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25700.15286263537562572No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG300.001309260839.66426534-35
GTATCAA98700.032.3036421
GGTATCA72450.028.3789041
TCAACGC119200.026.786894
ATCAACG119950.026.668273
TATCAAC121150.026.4509242
CAACGCA120850.026.2404485
AACGCAG122400.025.903536
GAGTACT64000.024.26626412-13
CATGGGG47100.024.03074
AGTACTT69000.023.58580212-13
ACGCAGA135650.023.0245637
CGCAGAG138250.022.5498628
GTACTTT69450.022.44767814-15
TACATGG111200.021.7471942
GTACATG111850.021.6856941
TATAGCG1400.00315736821.2487145
GCAGAGT142550.021.2442429
GTAATAC3401.0550139E-921.0243763
TACTTTT78950.020.72635514-15
Produced by FastQC (version 0.11.5)