##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1681248
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.020654894459355	31.0	30.0	34.0	26.0	34.0
2	30.115658130150937	31.0	30.0	34.0	26.0	34.0
3	30.219703458383297	31.0	30.0	34.0	26.0	34.0
4	33.76688210186718	35.0	35.0	37.0	29.0	37.0
5	33.28266799425189	35.0	33.0	37.0	27.0	37.0
6	33.041670235444144	35.0	33.0	37.0	26.0	37.0
7	32.89341489179467	35.0	33.0	37.0	26.0	37.0
8	32.816869373227505	35.0	33.0	37.0	25.0	37.0
9	34.13224104950608	38.0	34.0	39.0	25.0	39.0
10-11	34.03452316374503	38.0	33.5	39.0	25.0	39.0
12-13	33.93974922200651	38.0	33.5	39.0	25.0	39.0
14-15	34.82369228097223	38.5	33.5	41.0	25.0	41.0
16-17	34.82453005148557	38.5	33.5	41.0	24.5	41.0
18-19	34.90207363815452	39.0	34.0	41.0	25.0	41.0
20-21	34.88493577390129	39.0	34.0	41.0	24.5	41.0
22-23	34.847956993852186	39.0	34.0	41.0	24.0	41.0
24-25	34.81447472353871	39.0	34.0	41.0	24.0	41.0
26-27	34.652301742515085	39.0	34.0	41.0	23.5	41.0
28-29	34.524726869563565	39.0	33.5	41.0	23.0	41.0
30-31	34.32398075715183	38.0	33.0	41.0	22.0	41.0
32-33	34.12776401815793	38.0	33.0	40.5	20.5	41.0
34-35	33.97709856011724	38.0	33.0	40.0	19.0	41.0
36-37	33.812356356706445	38.0	33.0	40.0	18.0	41.0
38-39	33.64897028873789	38.0	33.0	40.0	16.0	41.0
40-41	33.44418372542302	38.0	32.5	40.0	15.0	41.0
42-43	33.26385220978702	38.0	32.0	40.0	14.0	41.0
44-45	32.99772490435676	38.0	32.0	40.0	9.0	41.0
46-47	32.761707374521784	38.0	31.5	40.0	7.5	41.0
48-49	32.49236341098993	38.0	31.0	40.0	2.0	41.0
50-51	32.279245536648965	37.5	31.0	40.0	2.0	41.0
52-53	32.036158853423174	37.0	31.0	40.0	2.0	41.0
54-55	31.847639670054626	37.0	30.5	40.0	2.0	41.0
56-57	31.708797869201927	37.0	30.0	40.0	2.0	41.0
58-59	31.640365966234608	36.5	30.0	40.0	2.0	41.0
60-61	31.378904539960793	36.0	30.0	40.0	2.0	41.0
62-63	31.12394512885666	36.0	30.0	39.5	2.0	41.0
64-65	30.86099581977198	35.0	29.5	39.0	2.0	41.0
66-67	30.550327197415253	35.0	29.0	39.0	2.0	41.0
68-69	30.240361326823884	35.0	29.0	38.5	2.0	40.0
70-71	29.896020099354764	35.0	29.0	37.5	2.0	40.0
72-73	29.621128173832773	35.0	29.0	37.0	2.0	39.5
74-75	29.366948838006053	35.0	29.0	36.5	2.0	39.0
76-77	29.03068315917699	34.0	28.5	36.0	2.0	39.0
78-79	28.700841874607434	34.0	28.0	36.0	2.0	38.0
80-81	28.445897928205717	34.0	28.0	35.0	2.0	37.0
82-83	28.192537032014314	34.0	27.0	35.0	2.0	37.0
84-85	27.987096192828183	34.0	27.0	35.0	2.0	36.0
86-87	27.745517912883763	34.0	27.0	35.0	2.0	36.0
88-89	27.608446820457182	34.0	26.5	35.0	2.0	36.0
90-91	27.420362433144902	34.0	26.0	35.0	2.0	35.0
92-93	27.544799458497497	34.0	27.0	35.0	2.0	35.0
94-95	27.485936042749195	34.0	27.0	35.0	2.0	35.0
96-97	27.422439759036145	34.0	27.0	35.0	2.0	35.0
98-99	27.366575900759436	34.0	27.0	35.0	2.0	35.0
100-101	27.233348381678372	34.0	26.0	35.0	2.0	35.0
102-103	25.95518567159634	31.5	24.0	34.0	2.0	35.0
104-105	26.988220655132377	33.5	25.0	35.0	2.0	35.0
106-107	27.171581765450426	34.0	26.0	35.0	2.0	35.0
108-109	27.156459665676927	34.0	26.0	35.0	2.0	35.0
110-111	27.096970821675328	34.0	26.0	35.0	2.0	35.0
112-113	27.00169041093283	34.0	25.0	35.0	2.0	35.0
114-115	26.910586064637698	34.0	25.0	35.0	2.0	35.0
116-117	26.78076226707779	34.0	25.0	35.0	2.0	35.0
118-119	26.654070963950588	34.0	25.0	35.0	2.0	35.0
120-121	26.518956453777193	34.0	25.0	35.0	2.0	35.0
122-123	26.288755733835817	34.0	25.0	35.0	2.0	35.0
124-125	25.20126715392375	32.5	21.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54569.0
3	18399.0
4	8915.0
5	7079.0
6	8103.0
7	10079.0
8	11290.0
9	11999.0
10	13062.0
11	14039.0
12	15059.0
13	16596.0
14	18717.0
15	15672.0
16	10789.0
17	9603.0
18	8773.0
19	8241.0
20	7915.0
21	8338.0
22	9222.0
23	11830.0
24	15756.0
25	21025.0
26	25425.0
27	29363.0
28	34619.0
29	40973.0
30	47650.0
31	56726.0
32	68351.0
33	85561.0
34	109058.0
35	150679.0
36	240554.0
37	336846.0
38	120013.0
39	360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.146452010674075	20.075496985119607	14.352877883656326	24.425173120549985
2	15.432771032453887	20.962892424492058	38.96524425024997	24.63909229280409
3	18.57172342866796	25.249509130675037	30.001766594238287	26.177000846418718
4	12.749873592920965	16.95707042884309	36.41482723770308	33.87822874053287
5	13.142119136668734	38.14561412797438	34.331760636820135	14.380506098536744
6	31.99501073334725	37.04007056826367	16.80933185582518	14.155586842563894
7	27.50818474686767	32.89738849472037	21.223179488643666	18.371247269768304
8	26.27037734333872	35.42423241994058	19.636724872776377	18.668665363944335
9	26.45991274761716	15.633739888426893	19.509349616954545	38.39699774700141
10-11	24.808158962586017	26.333606789973995	27.567612104828882	21.29062214261111
12-13	25.79729966486004	23.81388503274508	27.958858721566127	22.429956580828755
14-15	23.090208985557947	24.99863159260013	25.806467926315424	26.1046914955265
16-17	22.33307995650447	28.0755905886623	26.63396072609568	22.957368728737542
18-19	22.456261338909098	26.575219548651173	29.183741350595348	21.78477776184438
20-21	23.382813308727204	25.712959029842665	28.99537266744483	21.9088549939853
22-23	22.85602522533242	25.543861736621352	28.807418865454977	22.79269417259125
24-25	22.509164172169534	25.957445162956127	28.82266378680282	22.71072687807152
26-27	22.39898887741358	26.009129611209207	28.891090746407883	22.700790764969327
28-29	22.37776008228898	25.985964112795156	29.137777227809654	22.498498577106208
30-31	22.70178214994333	25.964208265534687	28.85268333893571	22.48132624558627
32-33	22.488369623321258	25.939361704342872	28.802164161811195	22.77010451052468
34-35	22.497119586898336	26.1055416678938	28.94490163162424	22.45243711358362
36-37	22.87443665636045	25.905373440792427	28.66315047615534	22.557039426691773
38-39	22.796273824458734	25.999216400027485	28.487785925485415	22.716723850028366
40-41	22.61786434767294	26.0712161775814	28.64060585307718	22.67031362166848
42-43	22.931905834585788	25.869517618233335	28.377219756982498	22.821356790198383
44-45	22.928063471570027	26.081323692810216	28.139746928830984	22.850865906788776
46-47	22.991665191501262	26.170822666275935	27.930459463198464	22.907052679024336
48-49	23.059284184547575	26.441095561948956	27.53113013925823	22.96849011424524
50-51	22.904818210888756	26.753913541411034	27.32362105764154	23.017647190058675
52-53	23.08460499788936	26.609062099234905	27.1588400432064	23.147492859669335
54-55	22.927073937536147	26.660363797333993	27.13885034711397	23.273711918015895
56-57	23.065685396667686	26.631187846372146	26.94874212807477	23.35438462888539
58-59	22.741382792924355	26.41286379325783	27.397853198471307	23.44790021534651
60-61	22.967606844396286	26.601516791905684	27.29401313172514	23.136863231972885
62-63	22.578733199160688	26.73024990151284	27.327064273140913	23.363952626185565
64-65	22.569046612432423	26.594961945482716	27.36869696489154	23.467294477193324
66-67	22.884634322161602	26.641449864946114	27.10246869394758	23.3714471189447
68-69	23.110845621483715	26.27972478985597	27.187086494616697	23.42234309404362
70-71	22.712885796982793	26.28424955545751	27.398424607468314	23.604440040091383
72-73	22.875185797402587	26.36826622015787	27.179497249186895	23.577050733252648
74-75	22.948947571482414	26.472372171112184	27.052286922602576	23.52639333480283
76-77	22.886353529449384	26.28288245679007	27.2803882461252	23.550375767635348
78-79	22.9642293623614	26.3744344833659	27.191443890992428	23.469892263280272
80-81	22.841488213589205	26.408556704588314	27.102111213837894	23.647843867984584
82-83	22.78961503069812	26.378187363686646	26.95067743518022	23.88152017043502
84-85	22.859752034648515	26.455487601732425	26.760893103612393	23.923867260006663
86-87	22.87685471769736	26.305665330329848	26.890000321292025	23.92747963068077
88-89	22.766744212336306	26.194421471622448	26.966645445728936	24.072188870312306
90-91	22.63664179137779	26.358593874342358	26.995494483754705	24.009269850525147
92-93	22.704652524945434	26.214906606513434	27.034090831341995	24.046350037199137
94-95	22.45170164185267	26.24635166987327	27.132153731380576	24.169792956893485
96-97	22.719855415694752	26.016370436602482	27.086118205284254	24.177655942418514
98-99	22.687417303646733	26.13594447564563	26.84204639562887	24.334591825078768
100-101	22.465524903754112	26.16507947462327	27.052040900710413	24.317354720912203
102-103	22.51049348377712	25.989332492466406	27.04492787682093	24.455246146935547
104-105	22.578876664929453	26.062615863859516	26.7779376173512	24.580569853859828
106-107	22.325510147932118	26.152084389867387	26.885424366042386	24.63698109615811
108-109	22.39096402941039	26.078625413117006	26.91852712465805	24.611883432814555
110-111	22.309877036038454	26.149548545153518	26.82249201171516	24.71808240709287
112-113	22.243865672991355	26.203992547435146	26.744047704029427	24.80809407554407
114-115	22.209488570200577	26.32954050287846	26.640363231307134	24.82060769561383
116-117	22.225688984252937	26.237274645183184	26.620548486698887	24.916487883864992
118-119	22.24623064463457	26.16724058858275	26.617356374280764	24.969172392501918
120-121	22.09059691487014	26.158737919806608	26.71261110625336	25.038054059069893
122-123	21.985476850161017	26.16876380728275	26.61247054148936	25.233288801066873
124-125	21.837813516611572	26.267784936918748	26.6302685253188	25.264133021150876
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	55.5
2	135.5
3	275.0
4	235.0
5	138.0
6	218.0
7	520.0
8	827.0
9	883.0
10	722.5
11	596.5
12	654.0
13	737.0
14	863.5
15	1002.0
16	1146.5
17	1383.5
18	1585.0
19	1735.5
20	1967.5
21	2360.5
22	2898.5
23	3664.0
24	4546.5
25	5511.5
26	7019.5
27	9069.0
28	11235.0
29	13659.0
30	16589.0
31	19758.5
32	23391.5
33	26617.5
34	29347.0
35	32727.5
36	36877.0
37	40298.5
38	42617.5
39	44841.5
40	47256.5
41	49624.0
42	51721.0
43	54737.5
44	57291.5
45	60170.5
46	63388.5
47	65205.5
48	66731.5
49	68043.5
50	71986.0
51	71311.5
52	65376.5
53	62808.0
54	60662.5
55	56936.5
56	51460.5
57	45453.0
58	40969.5
59	37107.5
60	32415.0
61	27336.0
62	22330.0
63	16666.5
64	11927.0
65	9110.0
66	6675.5
67	4614.0
68	3236.5
69	2374.5
70	1794.0
71	1346.5
72	1057.5
73	982.0
74	789.0
75	565.5
76	388.5
77	237.0
78	158.5
79	89.5
80	56.5
81	33.5
82	16.0
83	16.0
84	11.0
85	4.0
86	5.0
87	5.0
88	2.0
89	3.0
90	4.0
91	1.0
92	1.0
93	1.5
94	1.0
95	4.0
96	5.0
97	2.0
98	1.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05448333618835532
2	5.9479624659776546E-5
3	0.0027955423590094977
4	0.12782171339385978
5	0.016118978282799443
6	0.0023197053617312856
7	0.0042825329755039115
8	0.009100382572945811
9	0.02242381849673576
10-11	0.017784407773273187
12-13	0.018349464207541064
14-15	0.027479586592816765
16-17	0.036609708978092466
18-19	0.03812643940691677
20-21	0.021769542625478217
22-23	0.023672890614591064
24-25	0.022691476807704752
26-27	0.02046099088296313
28-29	0.020371771445973467
30-31	0.02456508498448771
32-33	0.024535345172157823
34-35	0.02971007251755839
36-37	0.03250561487656788
38-39	0.032327176002588554
40-41	0.034290003616361184
42-43	0.03247587506423799
44-45	0.029472154018919283
46-47	0.03839409771788576
48-49	0.03101862426007347
50-51	0.022929395306343858
52-53	0.028461000399703076
54-55	0.03164316031900112
56-57	0.025754677477683247
58-59	0.027866204153105312
60-61	0.03934577171244218
62-63	0.04913016996897542
64-65	0.04154651782485392
66-67	0.0370260663507109
68-69	0.029501893831249168
70-71	0.034944279487618725
72-73	0.03889967452749386
74-75	0.023643150802261176
76-77	0.021145006566550564
78-79	0.028788138335331847
80-81	0.024624564609147487
82-83	0.036133871980814254
84-85	0.02188850187479777
86-87	0.031762119568320674
88-89	0.03081044557376425
90-91	0.03155394088201146
92-93	0.02554649879137403
94-95	0.02034203163364358
96-97	0.017308570775994975
98-99	0.023791849863910617
100-101	0.023613410989931288
102-103	0.026349473724281012
104-105	0.02355393136527151
106-107	0.014304849730676259
108-109	0.01650559584308799
110-111	0.020758389006262017
112-113	0.012847598926511735
114-115	0.013412655360779612
116-117	0.012163583242924303
118-119	0.0074349530824720685
120-121	0.00517472734540056
122-123	0.004163573726184359
124-125	0.06150193189820895
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1681248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.79262449624998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.16413409252935	33.74699623385066
2	14.102743282718311	13.762197147213726
3	6.280807876786637	9.193713007954187
4	3.2839861913200687	6.409372203358011
5	1.9374106484466191	4.7265675132346034
6	1.2884772653533347	3.772091242820418
7	0.8540737286425789	2.9170749113638617
8	0.5911557891331551	2.30752339503667
9	0.4396879924441338	1.9308177999752953
>10	1.972024770051362	15.725847703158651
>50	0.058831084514950734	1.9274161555995195
>100	0.02457835427549436	2.147270253539596
>500	0.001351656566130728	0.4673518560187649
>1k	7.37267217889488E-4	0.9657605768762366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3980	0.2367289061459107	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3119	0.18551694931384305	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2692	0.16011914958411846	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2608	0.15512286111269724	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2570	0.15286263537562572	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.9479624659776546E-5
6	0.0	0.0	0.0	0.0	1.1895924931955309E-4
7	0.0	0.0	0.0	0.0	1.1895924931955309E-4
8	0.0	0.0	0.0	0.0	1.1895924931955309E-4
9	0.0	0.0	0.0	0.0	1.1895924931955309E-4
10-11	0.0	0.0	0.0	0.0	1.1895924931955309E-4
12-13	0.0	0.0	0.0	0.0	1.7843887397932964E-4
14-15	0.0	0.0	0.0	0.0	2.081786863092179E-4
16-17	0.0	0.0	0.0	8.921943698966482E-5	2.9739812329888276E-4
18-19	0.0	0.0	0.0	2.3791849863910618E-4	3.568777479586593E-4
20-21	0.0	0.0	0.0	4.7583699727821237E-4	4.4609718494832413E-4
22-23	0.0	0.0	0.0	0.0013382915548449722	4.7583699727821237E-4
24-25	0.0	0.0	0.0	0.002646843297360056	5.353166219379889E-4
26-27	0.0	0.0	0.0	0.0043122727878338	5.650564342678773E-4
28-29	0.0	0.0	0.0	0.0074349530824720685	6.54275871257542E-4
30-31	0.0	0.0	0.0	0.016802993966386875	6.840156835874302E-4
32-33	0.0	0.0	0.0	0.03310041112316565	7.137554959173185E-4
34-35	0.0	0.0	0.0	0.05632720455280839	7.137554959173185E-4
36-37	0.0	0.0	0.0	0.09368040883914806	7.137554959173185E-4
38-39	0.0	0.0	0.0	0.15378456955785227	7.137554959173185E-4
40-41	0.0	0.0	0.0	0.2234054702221207	7.137554959173185E-4
42-43	0.0	0.0	0.0	0.2880598222272978	7.137554959173185E-4
44-45	0.0	0.0	0.0	0.358543177449133	7.434953082472069E-4
46-47	0.0	0.0	0.0	0.4455618683263861	7.732351205770951E-4
48-49	0.0	0.0	0.0	0.5328184777022783	8.327147452368717E-4
50-51	0.0	0.0	0.0	0.6278371780962713	9.516739945564247E-4
52-53	0.0	0.0	0.0	0.7245510477930681	9.516739945564247E-4
54-55	0.0	0.0	0.0	0.8311385351833875	0.0010111536192162012
56-57	0.0	0.0	0.0	0.9558970479072688	0.0011003730562058661
58-59	0.0	0.0	0.0	1.0906481375739927	0.0011301128685357543
60-61	0.0	0.0	0.0	1.226023763299644	0.001189592493195531
62-63	0.0	0.0	0.0	1.3686856430461183	0.001189592493195531
64-65	0.0	0.0	0.0	1.5259795104588973	0.001308551742515084
66-67	0.0	0.0	0.0	1.7015336226422275	0.0013977711795047489
68-69	0.0	0.0	0.0	1.9069465064047662	0.0014869906164944138
70-71	0.0	0.0	0.0	2.131095471935134	0.0014869906164944138
72-73	0.0	0.0	0.0	2.3511403433639773	0.001516730428824302
74-75	0.0	0.0	0.0	2.5747539922724068	0.0015464702411541902
76-77	0.0	0.0	0.0	2.8245981556557984	0.0015464702411541902
78-79	0.0	0.0	0.0	3.1143531471859003	0.0015464702411541902
80-81	0.0	0.0	0.0	3.4221602048002437	0.0015762100534840785
82-83	0.0	0.0	0.0	3.7512609680427875	0.001754648927463408
84-85	0.0	0.0	0.0	4.085893336378691	0.0017843887397932963
86-87	0.0	0.0	0.0	4.435246911817888	0.0019330878014427377
88-89	0.0	0.0	0.0	4.831381212051999	0.0020817868630921793
90-91	0.0	0.0	0.0	5.267485820057481	0.0021115266754220675
92-93	0.0	0.0	0.0	5.7084380174727345	0.0022007461124117322
94-95	0.0	0.0	0.0	6.1545054626087285	0.0022304859247416205
96-97	0.0	0.0	0.0	6.627011600905994	0.002498144235710615
98-99	0.0	0.0	0.0	7.140380241344525	0.002676583109689945
100-101	0.0	0.0	0.0	7.705436675612402	0.002676583109689945
102-103	0.0	0.0	0.0	8.273734749424237	0.002676583109689945
104-105	0.0	0.0	0.0	8.850449190125431	0.002676583109689945
106-107	0.0	0.0	0.0	9.4388811168846	0.002676583109689945
108-109	0.0	0.0	0.0	10.051372551818648	0.002676583109689945
110-111	0.0	0.0	0.0	10.70823578674889	0.0027360627343497213
112-113	0.0	0.0	0.0	11.391805373151374	0.0027360627343497213
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	30	0.0013092608	39.664265	34-35
GTATCAA	9870	0.0	32.303642	1
GGTATCA	7245	0.0	28.378904	1
TCAACGC	11920	0.0	26.78689	4
ATCAACG	11995	0.0	26.66827	3
TATCAAC	12115	0.0	26.450924	2
CAACGCA	12085	0.0	26.240448	5
AACGCAG	12240	0.0	25.90353	6
GAGTACT	6400	0.0	24.266264	12-13
CATGGGG	4710	0.0	24.0307	4
AGTACTT	6900	0.0	23.585802	12-13
ACGCAGA	13565	0.0	23.024563	7
CGCAGAG	13825	0.0	22.549862	8
GTACTTT	6945	0.0	22.447678	14-15
TACATGG	11120	0.0	21.747194	2
GTACATG	11185	0.0	21.685694	1
TATAGCG	140	0.003157368	21.248714	5
GCAGAGT	14255	0.0	21.244242	9
GTAATAC	340	1.0550139E-9	21.024376	3
TACTTTT	7895	0.0	20.726355	14-15
>>END_MODULE