FastQCFastQC Report
Mon 15 Aug 2016
SRR2920969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999109
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54850.035.1590731
TCAACGC63350.029.6795544
CAACGCA63750.029.3999925
AACGCAG64850.029.2675746
CATGGGG50750.029.0758574
ATCAACG65300.028.7939783
GGTATCA37100.028.0758671
TATCAAC68250.027.985312
GTACATG89700.027.7365761
TACATGG86400.027.5469782
ACATGGG87950.026.655583
CGCAGAG72900.025.1372838
ACGCAGA73650.024.8819247
ATGGGGA28050.023.5454485
GTATAGG5200.021.7479971
GCAGAGT81050.021.6552969
GTGTACG3251.45028025E-820.1455141
GTGTAGC16500.019.4795461
TAGCCCT16250.019.406154
AGAGTAC73550.018.847910-11
Produced by FastQC (version 0.11.5)