FastQCFastQC Report
Mon 15 Aug 2016
SRR2920969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999109
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35590.17802931205852207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27900.13956217494893974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21760.10884849200318743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69250.043.193331
GGTATCA43050.036.5395771
TCAACGC87900.033.3426554
ATCAACG88200.033.2292443
CAACGCA89550.032.688175
TATCAAC91450.032.2364732
AACGCAG92250.031.8564476
ACGCAGA106500.027.428457
CGCAGAG106650.027.279678
GTACATG87900.025.6233391
CATGGGG51700.025.5791664
TACATGG86750.025.264132
GCAGAGT114900.024.544239
AGTACTT65100.023.80951112-13
ACATGGG90800.023.7490733
ACCGTCG1552.1078723E-423.0308888
GAGTACT60550.022.79804212-13
AGAGTAC106700.022.52865410-11
ATGGGGC19900.021.5263565
CTATACT8800.020.984714
Produced by FastQC (version 0.11.5)