##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920969_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1999109
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.71104076866244	31.0	30.0	34.0	26.0	34.0
2	29.805863512194684	31.0	30.0	34.0	26.0	34.0
3	29.920141423003948	31.0	30.0	34.0	26.0	34.0
4	33.43199045174625	35.0	33.0	37.0	28.0	37.0
5	32.91608761703339	35.0	33.0	37.0	26.0	37.0
6	32.67221447154707	35.0	33.0	37.0	25.0	37.0
7	32.50120128517254	35.0	33.0	37.0	25.0	37.0
8	32.42966691661135	35.0	33.0	37.0	24.0	37.0
9	33.71952204707197	37.0	33.0	39.0	23.0	39.0
10-11	33.606586234167324	37.0	33.0	39.0	23.0	39.0
12-13	33.486904666028714	37.0	33.0	39.0	22.0	39.0
14-15	34.33797731889557	38.0	33.0	41.0	20.0	41.0
16-17	34.33507577625832	38.0	33.0	41.0	20.0	41.0
18-19	34.377142016768474	38.5	33.0	41.0	20.5	41.0
20-21	34.3633931416446	39.0	33.0	41.0	19.5	41.0
22-23	34.317120777306286	39.0	33.0	41.0	19.0	41.0
24-25	34.283224176370574	38.5	33.0	41.0	19.0	41.0
26-27	34.126963562266994	38.0	33.0	41.0	18.5	41.0
28-29	33.99909509686566	38.0	33.0	40.5	18.0	41.0
30-31	33.81232514085025	38.0	33.0	40.0	17.5	41.0
32-33	33.64731062688428	38.0	33.0	40.0	16.5	41.0
34-35	33.49979215740613	38.0	32.0	40.0	16.0	41.0
36-37	33.32944026563834	38.0	32.0	40.0	15.0	41.0
38-39	33.17789225099782	38.0	32.0	40.0	11.5	41.0
40-41	32.976854438652424	38.0	32.0	40.0	9.0	41.0
42-43	32.805465334806655	38.0	31.0	40.0	8.5	41.0
44-45	32.568717613696904	38.0	31.0	40.0	7.5	41.0
46-47	32.37332231509137	38.0	31.0	40.0	2.0	41.0
48-49	32.125881830355425	37.5	31.0	40.0	2.0	41.0
50-51	31.913318383339778	37.0	30.0	40.0	2.0	41.0
52-53	31.682328977559504	37.0	30.0	40.0	2.0	41.0
54-55	31.494671376098054	37.0	30.0	40.0	2.0	41.0
56-57	31.368292574341872	37.0	30.0	40.0	2.0	41.0
58-59	31.30776635991334	36.0	30.0	40.0	2.0	41.0
60-61	31.053992053459815	36.0	29.5	40.0	2.0	41.0
62-63	30.80931605030041	35.5	29.0	39.0	2.0	41.0
64-65	30.56038940347925	35.0	29.0	39.0	2.0	41.0
66-67	30.266811614574294	35.0	29.0	39.0	2.0	41.0
68-69	29.965694967107847	35.0	28.5	38.5	2.0	40.0
70-71	29.62689778296231	35.0	28.0	37.5	2.0	40.0
72-73	29.356315488550152	35.0	28.0	37.0	2.0	39.5
74-75	29.089771743311644	34.0	28.0	37.0	2.0	39.0
76-77	28.756100842925523	34.0	28.0	36.0	2.0	39.0
78-79	28.438127935995485	34.0	27.5	36.0	2.0	38.5
80-81	28.174062794975164	34.0	27.0	35.0	2.0	37.0
82-83	27.906566125208784	34.0	26.0	35.0	2.0	37.0
84-85	27.694576183689833	34.0	26.0	35.0	2.0	36.0
86-87	27.455334601565	34.0	26.0	35.0	2.0	36.0
88-89	27.317590486561762	34.0	26.0	35.0	2.0	36.0
90-91	27.119088553950785	34.0	26.0	35.0	2.0	35.0
92-93	27.240554416992772	34.0	26.0	35.0	2.0	35.0
94-95	27.172606396149483	34.0	26.0	35.0	2.0	35.0
96-97	27.10939248435178	34.0	26.0	35.0	2.0	35.0
98-99	27.04969538929593	34.0	25.5	35.0	2.0	35.0
100-101	26.913178571053404	34.0	25.0	35.0	2.0	35.0
102-103	25.644746484558873	31.5	23.5	34.0	2.0	35.0
104-105	26.680151757608016	33.5	25.0	35.0	2.0	35.0
106-107	26.87077192889432	34.0	25.0	35.0	2.0	35.0
108-109	26.854971389754134	34.0	25.0	35.0	2.0	35.0
110-111	26.791698951883063	34.0	25.0	35.0	2.0	35.0
112-113	26.685076701670596	34.0	25.0	35.0	2.0	35.0
114-115	26.604660376197597	34.0	25.0	35.0	2.0	35.0
116-117	26.475494082613807	34.0	25.0	35.0	2.0	35.0
118-119	26.349624757829616	34.0	25.0	35.0	2.0	35.0
120-121	26.213276264575867	34.0	24.0	35.0	2.0	35.0
122-123	25.98049055854383	34.0	23.5	35.0	2.0	35.0
124-125	24.881549480293472	32.5	17.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79325.0
3	24798.0
4	11965.0
5	9243.0
6	10273.0
7	12312.0
8	13471.0
9	14502.0
10	15551.0
11	16386.0
12	17198.0
13	18719.0
14	20547.0
15	17460.0
16	12800.0
17	11274.0
18	10313.0
19	9518.0
20	9304.0
21	9615.0
22	11231.0
23	14450.0
24	19485.0
25	25869.0
26	31369.0
27	36015.0
28	42675.0
29	49811.0
30	58618.0
31	69374.0
32	84277.0
33	103891.0
34	132544.0
35	180567.0
36	280163.0
37	382294.0
38	131561.0
39	341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19282451512226	20.077464327021776	13.855994827036541	24.87371633081942
2	15.533885880045439	21.024387388969174	38.878559276717056	24.56316745426833
3	18.740689383320777	25.38140387841898	29.85135028328286	26.026556454977385
4	12.896325338376593	17.0044756264675	36.686363563512224	33.41283547164368
5	13.192826117191963	38.161632494599274	34.668697212235706	13.976844175973055
6	32.00351966832278	37.44669973567471	16.832228476297765	13.71755211970474
7	27.394124456352632	32.83334950810356	21.506124568408772	18.266401467135037
8	25.941058580214982	36.06489879761825	19.562141734363383	18.431900887803383
9	26.195613124800488	15.690398969677744	19.66613596130888	38.44785194421289
10-11	24.947572106334253	26.484347931590445	27.78619575998869	20.78188420208661
12-13	25.687852838455367	23.772109041795385	28.112502041950737	22.42753607779851
14-15	22.74584683404674	25.10023114772514	25.82799997297981	26.325922045248312
16-17	22.439829577185684	28.17636179118101	26.416011617735663	22.967797013897645
18-19	22.457169308528606	26.901752761757013	28.73575959264866	21.90531833706572
20-21	23.341226210355806	26.025329755896205	28.600909369507978	22.032534664240014
22-23	22.812257173893137	25.918705134241403	28.409397373462735	22.85964031840272
24-25	22.4953368103783	26.20975247068995	28.611895536547	22.68301518238475
26-27	22.363494405136233	26.35068384447907	28.575320457895987	22.710501292488704
28-29	22.45534148509982	26.384810476182373	28.66550555945538	22.49434247926243
30-31	22.80754160355895	26.21218102770635	28.47542764390686	22.50484972482784
32-33	22.521333843694414	26.31819512304956	28.46298756370132	22.69748346955471
34-35	22.617249245558792	26.453978711022625	28.517652498199897	22.411119545218686
36-37	22.880677442647002	26.13638922428654	28.32004891774004	22.662884415326424
38-39	22.774481176178302	26.386025889713395	28.242875625144332	22.596617308963975
40-41	22.859669885082777	26.478999397016107	28.182341328916454	22.47898938898466
42-43	22.838218133708025	26.306941800720963	28.120352634349523	22.73448743122149
44-45	22.84304726077829	26.402247889851193	27.942405703487598	22.812299145882918
46-47	22.95960660748498	26.515312067525077	27.638361324909884	22.886720000080068
48-49	22.93226056565494	26.698414692026724	27.40829179894663	22.961032943371706
50-51	22.7849105128064	27.009871860943356	27.274680903227743	22.930536723022502
52-53	22.994497074655396	26.844168012479468	27.159432219196315	23.00190269366882
54-55	22.986851010224214	26.86136829055627	27.110061960751935	23.04171873846758
56-57	22.952378164671362	26.89897794346303	27.037126823523938	23.111517068341666
58-59	22.791535391750703	26.753913353927018	27.36677008540615	23.087781168916134
60-61	22.85223075348639	26.83589963523466	27.442348910633243	22.869520700645708
62-63	22.77507395170749	26.78717147762531	27.42653062705493	23.011223943612276
64-65	22.690556344304817	26.731635473398857	27.381381925403392	23.196426256892938
66-67	22.79143782918194	26.91121314100559	27.202582162561146	23.094766867251323
68-69	22.97774277369443	26.63956963658189	27.245449955641664	23.137237634082013
70-71	22.712602805738783	26.47895020362861	27.374605834709666	23.433841155922945
72-73	23.01472580743229	26.553193235531598	27.149651865628417	23.282429091407696
74-75	22.81531283046595	26.64494644622219	27.098464248822363	23.4412764744895
76-77	22.819018104093537	26.560842642885447	27.248401932490907	23.371737320530116
78-79	22.870582430850817	26.659632949022477	27.092589377168224	23.377195242958486
80-81	22.844869965063	26.66162146205026	26.919302160640147	23.574206412246586
82-83	22.78999511595601	26.63885697357412	27.118203872806685	23.452944037663183
84-85	22.89819903885363	26.659278364700263	26.877650219772796	23.564872376673314
86-87	22.746507187783582	26.524306260103263	27.065132025186827	23.66405452692632
88-89	22.782377210166956	26.46383294928281	26.997750739954014	23.756039100596215
90-91	22.683946118015648	26.594165495524052	26.986578543032703	23.735309843427594
92-93	22.633149676881228	26.51507816719817	27.121862473065093	23.729909682855503
94-95	22.49700547270502	26.55170050123934	27.128516005335612	23.82277802072003
96-97	22.59558281275485	26.327612723628608	27.131119736553767	23.945684727062776
98-99	22.621830844441842	26.343497297206692	26.933616134051142	24.10105572430032
100-101	22.46703253953581	26.409818475387393	27.08831859509622	24.034830389980584
102-103	22.380902225203293	26.268496972659744	27.160674823802317	24.18992597833465
104-105	22.344599461173225	26.37614351835521	26.95347221544665	24.325784805024913
106-107	22.30624740626339	26.42605796311847	26.940039938853484	24.327654691764657
108-109	22.256466009469463	26.29821129856527	26.97946531828419	24.465857373681075
110-111	22.285695558806637	26.38495846200339	26.90223210443839	24.427113874751583
112-113	22.15685097356467	26.47863260691201	26.82916091332973	24.535355506193593
114-115	22.13102267044862	26.543538933400505	26.741758707699127	24.58367968845175
116-117	22.15755947534373	26.412918541198426	26.862280856449956	24.56724112700789
118-119	22.16985320583675	26.314917965907696	26.719904470109512	24.795324358146043
120-121	22.05213234460984	26.498061413945962	26.731402092587402	24.718404148856795
122-123	21.985903233552104	26.433713182327516	26.702217064190815	24.878166519929565
124-125	21.879242701083854	26.525708156472007	26.596957380350982	24.99809176209316
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	57.5
2	147.5
3	282.0
4	242.0
5	161.5
6	242.0
7	484.0
8	768.5
9	833.5
10	675.0
11	576.0
12	637.5
13	731.5
14	869.5
15	1012.0
16	1174.0
17	1364.5
18	1586.0
19	1887.5
20	2180.5
21	2632.5
22	3294.0
23	4254.5
24	5463.5
25	6847.5
26	8979.0
27	11830.0
28	14686.5
29	17465.5
30	20423.5
31	24629.0
32	29106.5
33	33080.5
34	37147.0
35	40680.5
36	45189.5
37	49346.0
38	52376.5
39	55278.0
40	58977.0
41	62047.5
42	63484.0
43	67173.5
44	70839.5
45	72824.5
46	75160.0
47	77224.0
48	78731.5
49	79425.0
50	79926.0
51	78017.0
52	73546.0
53	70963.0
54	68435.0
55	64331.0
56	59785.5
57	54477.5
58	49870.5
59	44803.5
60	37803.0
61	30839.0
62	24726.0
63	18514.0
64	13433.5
65	10503.5
66	8037.5
67	5975.5
68	4556.5
69	3414.5
70	2698.5
71	2230.0
72	1843.0
73	1669.0
74	1323.5
75	951.0
76	689.0
77	434.5
78	272.0
79	160.0
80	101.0
81	75.0
82	50.0
83	32.0
84	24.5
85	18.5
86	11.5
87	5.5
88	3.5
89	1.0
90	1.5
91	1.5
92	3.0
93	4.5
94	2.5
95	2.5
96	3.5
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05142290890591759
2	1.0004456985587079E-4
3	0.0023510473916129636
4	0.1259060911636134
5	0.015156752333164424
6	0.0028512702408923173
7	0.005552473627000829
8	0.012155415237488301
9	0.02556138759817499
10-11	0.020584170247845417
12-13	0.020384081108133674
14-15	0.03018844895400901
16-17	0.039267493668429285
18-19	0.04171858562989812
20-21	0.02358550734352154
22-23	0.025411320743391183
24-25	0.02386062991062518
26-27	0.02228492793539522
28-29	0.022109849938147443
30-31	0.027112078430940986
32-33	0.026211677302238147
34-35	0.030513593806040594
36-37	0.03261452977301388
38-39	0.03353994204418068
40-41	0.035715911438545876
42-43	0.03326481947707704
44-45	0.03063864951836043
46-47	0.03999281679988435
48-49	0.03323980833461307
50-51	0.02496112017903976
52-53	0.03133896150735153
54-55	0.03333985290446894
56-57	0.027037045003549084
58-59	0.029988359814297268
60-61	0.04191867476960986
62-63	0.05074760805939046
64-65	0.04341934331744792
66-67	0.03921747138350135
68-69	0.030613638375896465
70-71	0.03704150198913616
72-73	0.04074315107380338
74-75	0.02553637645571102
76-77	0.022259916792931253
78-79	0.03201426235387865
80-81	0.025586398740638956
82-83	0.03826704796987058
84-85	0.02271011735728267
86-87	0.03419023174824384
88-89	0.033715020041428456
90-91	0.03524069973173048
92-93	0.027037045003549084
94-95	0.023160317921634088
96-97	0.01855826770826403
98-99	0.026436777584413856
100-101	0.025486354170783085
102-103	0.02863775812124301
104-105	0.025386309600927214
106-107	0.015181763475628392
108-109	0.018433211995944193
110-111	0.022860184212066474
112-113	0.01345599464561462
114-115	0.015882075464619488
116-117	0.013831161782574137
118-119	0.007678420736438083
120-121	0.005027239635257507
122-123	0.0034515376600275424
124-125	0.059651574776562966
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1999109.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.62659832312969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57136048198545	37.66557239525367
2	13.917365549964323	14.648472130282705
3	5.8384112393130545	9.21767169409722
4	2.875604866033663	6.05333208883163
5	1.6494940105570808	4.340362936499788
6	1.0247142067635349	3.2356333773209416
7	0.7012829219431981	2.5834294250782777
8	0.4927536520863753	2.0745558816483896
9	0.3385026053863654	1.6032816580501017
>10	1.5037801500827157	13.236071618039032
>50	0.05906742580672645	2.137588029662454
>100	0.026702869153867246	2.472410480119035
>500	4.8001046182263065E-4	0.17334316250507353
>1k	4.800104618226264E-4	0.5582751226117895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3559	0.17802931205852207	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2790	0.13956217494893974	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2176	0.10884849200318743	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.0022284927935395E-5	0.0
3	0.0	0.0	0.0	5.0022284927935395E-5	0.0
4	0.0	0.0	0.0	2.0008913971174158E-4	0.0
5	0.0	0.0	0.0	2.0008913971174158E-4	0.0
6	0.0	0.0	0.0	2.0008913971174158E-4	5.0022284927935395E-5
7	0.0	0.0	0.0	2.0008913971174158E-4	5.0022284927935395E-5
8	0.0	0.0	0.0	2.0008913971174158E-4	1.0004456985587079E-4
9	0.0	0.0	0.0	2.5011142463967696E-4	1.5006685478380618E-4
10-11	0.0	0.0	0.0	3.5015599449554776E-4	1.5006685478380618E-4
12-13	0.0	0.0	0.0	3.5015599449554776E-4	1.5006685478380618E-4
14-15	0.0	0.0	0.0	4.0017827942348316E-4	2.0008913971174158E-4
16-17	0.0	0.0	0.0	4.5020056435141856E-4	2.2510028217570928E-4
18-19	0.0	0.0	0.0	5.75256276671257E-4	2.5011142463967696E-4
20-21	0.0	0.0	0.0	6.502897040631601E-4	2.751225671036447E-4
22-23	0.0	0.0	0.0	8.25367701310934E-4	4.2518942188745083E-4
24-25	0.0	0.0	0.0	0.0014756574053740941	5.502451342072894E-4
26-27	0.0	0.0	0.0	0.002075924824509319	5.502451342072894E-4
28-29	0.0	0.0	0.0	0.003701649084667219	5.502451342072894E-4
30-31	0.0	0.0	0.0	0.010404635265010561	5.502451342072894E-4
32-33	0.0	0.0	0.0	0.025561387598174987	5.502451342072894E-4
34-35	0.0	0.0	0.0	0.042919120468168565	7.003119889910955E-4
36-37	0.0	0.0	0.0	0.0702312880388213	7.003119889910955E-4
38-39	0.0	0.0	0.0	0.1180025701449996	7.003119889910955E-4
40-41	0.0	0.0	0.0	0.17432766297385485	7.003119889910955E-4
42-43	0.0	0.0	0.0	0.22475012618121373	7.003119889910955E-4
44-45	0.0	0.0	0.0	0.2822757538483394	7.003119889910955E-4
46-47	0.0	0.0	0.0	0.3529822535939761	8.25367701310934E-4
48-49	0.0	0.0	0.0	0.42714029099964035	9.004011287028371E-4
50-51	0.0	0.0	0.0	0.5106024734019006	9.004011287028371E-4
52-53	0.0	0.0	0.0	0.5920137421221154	9.754345560947402E-4
54-55	0.0	0.0	0.0	0.6850551920880752	0.001075479125950611
56-57	0.0	0.0	0.0	0.7922529486886407	0.0011004902684145787
58-59	0.0	0.0	0.0	0.9101554742637845	0.0011004902684145787
60-61	0.0	0.0	0.0	1.039888270224385	0.0011505125533425142
62-63	0.0	0.0	0.0	1.1665697068043812	0.0012505571231983849
64-65	0.0	0.0	0.0	1.3019800321043025	0.0013005794081263201
66-67	0.0	0.0	0.0	1.4569990930959742	0.0013506016930542556
68-69	0.0	0.0	5.0022284927935395E-5	1.6342030374531853	0.0013506016930542556
70-71	0.0	0.0	5.0022284927935395E-5	1.8316910183486743	0.001400623977982191
72-73	0.0	0.0	5.0022284927935395E-5	2.031154879498817	0.0014506462629101265
74-75	0.0	0.0	5.0022284927935395E-5	2.2335200331747793	0.0014506462629101265
76-77	0.0	0.0	5.0022284927935395E-5	2.455168777690461	0.0014506462629101265
78-79	0.0	0.0	5.0022284927935395E-5	2.713859024195279	0.0014506462629101265
80-81	0.0	0.0	5.0022284927935395E-5	2.994834198635492	0.001500668547838062
82-83	0.0	0.0	5.0022284927935395E-5	3.2883149443076887	0.001650735402621868
84-85	0.0	0.0	5.0022284927935395E-5	3.58824856473559	0.0016757465450858357
86-87	0.0	0.0	5.0022284927935395E-5	3.8966359513163114	0.0017257688300137712
88-89	0.0	0.0	5.0022284927935395E-5	4.241164438757466	0.0018008022574056743
90-91	0.0	0.0	5.0022284927935395E-5	4.618232422544244	0.0018008022574056743
92-93	0.0	0.0	5.0022284927935395E-5	5.018510746537583	0.0018008022574056743
94-95	0.0	0.0	5.0022284927935395E-5	5.432670254598424	0.001900846827261545
96-97	0.0	0.0	5.0022284927935395E-5	5.851706935439738	0.001900846827261545
98-99	0.0	0.0	5.0022284927935395E-5	6.2975805721448905	0.001900846827261545
100-101	0.0	0.0	5.0022284927935395E-5	6.80603208729489	0.001900846827261545
102-103	0.0	0.0	5.0022284927935395E-5	7.332916814440834	0.0019508691121894804
104-105	0.0	0.0	5.0022284927935395E-5	7.858801095888218	0.0020008913971174156
106-107	0.0	0.0	5.0022284927935395E-5	8.383609898209652	0.0020759248245093187
108-109	0.0	0.0	5.0022284927935395E-5	8.935405723249708	0.002150958251901222
110-111	0.0	0.0	5.0022284927935395E-5	9.534372562976806	0.0022760139642210605
112-113	0.0	0.0	5.0022284927935395E-5	10.16445326392908	0.0023510473916129636
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6925	0.0	43.19333	1
GGTATCA	4305	0.0	36.539577	1
TCAACGC	8790	0.0	33.342655	4
ATCAACG	8820	0.0	33.229244	3
CAACGCA	8955	0.0	32.68817	5
TATCAAC	9145	0.0	32.236473	2
AACGCAG	9225	0.0	31.856447	6
ACGCAGA	10650	0.0	27.42845	7
CGCAGAG	10665	0.0	27.27967	8
GTACATG	8790	0.0	25.623339	1
CATGGGG	5170	0.0	25.579166	4
TACATGG	8675	0.0	25.26413	2
GCAGAGT	11490	0.0	24.54423	9
AGTACTT	6510	0.0	23.809511	12-13
ACATGGG	9080	0.0	23.749073	3
ACCGTCG	155	2.1078723E-4	23.030888	8
GAGTACT	6055	0.0	22.798042	12-13
AGAGTAC	10670	0.0	22.528654	10-11
ATGGGGC	1990	0.0	21.526356	5
CTATACT	880	0.0	20.98471	4
>>END_MODULE