FastQCFastQC Report
Mon 15 Aug 2016
SRR2920968_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920968_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3243553
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73150.036.8593031
TCAACGC81700.031.8279574
ATCAACG85250.030.5733133
CAACGCA85400.030.3779135
GGTATCA50250.029.4935131
AACGCAG89150.029.3643556
TATCAAC90300.028.9957542
GTACATG139600.028.2677561
TACATGG139900.027.3503382
ACATGGG142150.026.6658443
CATGGGG93900.026.2351864
ACGCAGA104600.024.9133177
CGCAGAG107650.023.9311188
GCAGAGT116050.020.9169069
AGAGTAC101850.019.10157210-11
AAACGCG1600.006834162418.5942785
ATGGGGG46950.018.249725
ATGGGGA52650.018.0821865
GTATATA18900.017.9504891
CAGAGTA108450.017.41793310-11
Produced by FastQC (version 0.11.5)