FastQCFastQC Report
Mon 15 Aug 2016
SRR2920968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3243553
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34240.1055632511631535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86900.040.251761
GGTATCA57300.036.918181
TCAACGC106100.032.1143844
ATCAACG109000.031.2053173
CAACGCA110800.030.6077485
TATCAAC112900.030.4934062
AACGCAG115150.029.6540456
ACGCAGA136500.024.7990887
GTACATG134950.024.5509551
TACATGG136000.024.3067072
CGCAGAG142200.023.8890728
ACATGGG138350.022.992233
CATGGGG87450.022.410684
GCAGAGT153700.021.4056029
AGAGTAC136850.021.32408310-11
AGTACTT75700.020.35824412-13
GAGTACT67950.018.60826712-13
GTATAGG9350.018.4821681
CAGAGTA147050.018.2872989
GTACTTT73550.017.19145814-15
Produced by FastQC (version 0.11.5)