FastQCFastQC Report
Mon 15 Aug 2016
SRR2920967_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920967_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073832
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66550.035.5940131
GGTATCA44850.033.042951
TCAACGC73450.030.7021774
CAACGCA75650.030.121975
ATCAACG78750.028.7132973
TATCAAC82300.028.1982332
AACGCAG80950.027.9297686
GTACATG126900.026.3582521
ACGCAGA89450.024.9431617
ACATGGG126600.024.8640583
TACATGG130600.024.3307322
CATGGGG92200.024.1358414
CGCAGAG94250.023.6097188
GTATATA13900.021.8372151
GCAGAGT104750.020.2207169
AGAGTAC95100.018.30067610-11
CAGAGTA100400.017.27534110-11
GTACCGT5951.8189894E-1216.9993576
ATGGGGA50900.016.9489865
GTATAGC10950.016.8495961
Produced by FastQC (version 0.11.5)