##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920967_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3073832 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.221550494626904 31.0 28.0 33.0 16.0 34.0 2 28.286652295896456 31.0 28.0 34.0 16.0 34.0 3 28.360189821694874 31.0 28.0 34.0 16.0 34.0 4 31.793693994987365 35.0 32.0 37.0 19.0 37.0 5 31.8650212503481 35.0 32.0 37.0 19.0 37.0 6 31.885754979452358 35.0 32.0 37.0 18.0 37.0 7 31.685564142737796 35.0 32.0 37.0 17.0 37.0 8 31.690059834109345 35.0 32.0 37.0 17.0 37.0 9 32.8811587620924 37.0 32.0 39.0 17.0 39.0 10-11 32.80283161213755 37.0 32.0 39.0 17.0 39.0 12-13 32.748314644391755 37.0 32.0 39.0 17.0 39.0 14-15 33.60837287138659 38.0 32.0 40.0 16.5 41.0 16-17 33.45958041948942 38.0 32.0 40.0 14.5 41.0 18-19 33.551343567247656 38.0 32.0 40.0 16.0 41.0 20-21 33.590691846529026 38.0 32.0 40.0 16.0 41.0 22-23 33.52805407061935 38.0 32.0 40.0 16.0 41.0 24-25 33.449495287966286 38.0 32.0 40.0 15.5 41.0 26-27 33.313128694086075 38.0 32.0 40.0 11.5 41.0 28-29 33.237598053504556 38.0 32.0 40.0 10.0 41.0 30-31 33.106640180725556 38.0 32.0 40.0 10.0 41.0 32-33 33.029901438985604 38.0 31.5 40.0 9.0 41.0 34-35 32.86026155625942 38.0 31.0 40.0 9.0 41.0 36-37 32.70069330399319 38.0 31.0 40.0 8.0 41.0 38-39 32.56665588750459 38.0 31.0 40.0 8.0 41.0 40-41 32.48752875889118 38.0 31.0 40.0 4.5 41.0 42-43 32.26638313349591 38.0 30.0 40.0 2.0 41.0 44-45 32.08330627698586 37.5 30.0 40.0 2.0 41.0 46-47 31.88049688467034 37.0 30.0 40.0 2.0 41.0 48-49 31.71252316326982 37.0 30.0 40.0 2.0 41.0 50-51 31.47636256633414 37.0 30.0 40.0 2.0 41.0 52-53 31.291419960492313 36.5 29.5 40.0 2.0 41.0 54-55 31.072957630735836 36.0 29.0 40.0 2.0 41.0 56-57 30.86677069534054 36.0 29.0 39.5 2.0 41.0 58-59 30.606127465651994 35.5 29.0 39.0 2.0 41.0 60-61 30.411545263371583 35.0 28.5 39.0 2.0 41.0 62-63 30.292438233449325 35.0 28.0 39.0 2.0 41.0 64-65 30.061523531539784 35.0 28.0 39.0 2.0 40.0 66-67 29.797795552912454 35.0 28.0 38.5 2.0 40.0 68-69 29.491172907302676 34.5 28.0 38.0 2.0 40.0 70-71 29.16362816835793 34.0 28.0 37.0 2.0 40.0 72-73 28.882383780245636 34.0 28.0 37.0 2.0 39.0 74-75 28.57839920984621 34.0 27.0 36.0 2.0 39.0 76-77 28.303243963886118 34.0 27.0 36.0 2.0 38.5 78-79 27.98944216860258 34.0 26.0 35.0 2.0 37.0 80-81 27.673410745935367 34.0 26.0 35.0 2.0 37.0 82-83 27.41027957936543 33.5 26.0 35.0 2.0 36.5 84-85 27.17627736323911 33.0 26.0 35.0 2.0 36.0 86-87 27.112821390368765 33.0 26.0 35.0 2.0 36.0 88-89 27.208973684963915 34.0 26.0 35.0 2.0 35.5 90-91 27.123630211410383 34.0 26.0 35.0 2.0 35.0 92-93 26.975492642408565 34.0 26.0 35.0 2.0 35.0 94-95 26.898151720718634 33.5 25.5 35.0 2.0 35.0 96-97 26.808778586467966 33.0 25.0 35.0 2.0 35.0 98-99 26.741382905767132 33.0 25.0 35.0 2.0 35.0 100-101 26.56809757332216 33.0 25.0 35.0 2.0 35.0 102-103 25.21065708861122 31.5 21.5 34.0 2.0 35.0 104-105 26.238421130367566 33.0 24.5 35.0 2.0 35.0 106-107 26.437650951646024 33.0 25.0 35.0 2.0 35.0 108-109 26.433614459085597 33.0 25.0 35.0 2.0 35.0 110-111 26.32774448961427 33.0 24.5 35.0 2.0 35.0 112-113 26.235898058189257 33.0 24.0 35.0 2.0 35.0 114-115 26.10889550892827 33.0 24.0 35.0 2.0 35.0 116-117 25.982634542161055 33.0 23.5 35.0 2.0 35.0 118-119 25.788677455371666 33.0 23.0 35.0 2.0 35.0 120-121 25.57178629150845 33.0 19.5 35.0 2.0 35.0 122-123 25.353974290071804 33.0 18.5 35.0 2.0 35.0 124-125 24.197644503668386 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 185772.0 3 26459.0 4 17153.0 5 15061.0 6 16709.0 7 18489.0 8 20026.0 9 21298.0 10 22156.0 11 22504.0 12 22456.0 13 22611.0 14 22877.0 15 20498.0 16 17884.0 17 16242.0 18 14958.0 19 14493.0 20 15113.0 21 16277.0 22 19428.0 23 25515.0 24 35032.0 25 44744.0 26 52589.0 27 60426.0 28 69843.0 29 81275.0 30 94932.0 31 112132.0 32 136505.0 33 169166.0 34 217596.0 35 296069.0 36 447555.0 37 523582.0 38 138300.0 39 107.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50189963348171 18.617709908360666 13.132139897900801 24.748250560256814 2 15.867992546076978 19.889720464871257 39.24131896822972 25.000968020822047 3 19.48602172510987 24.15595715893198 28.739433397784538 27.61858771817361 4 13.047528711975648 16.087849941184988 36.02475599498242 34.83986535185694 5 14.31448168961017 37.45112527774443 33.87351350401621 14.360879528629193 6 33.457841700965844 35.57103532595523 17.089790151183156 13.881332821895775 7 28.91771779196991 31.777635350344376 20.76920574293439 18.535441114751325 8 26.766085535755067 35.201235529475916 19.357496042180305 18.67518289258871 9 26.520098589734747 16.169347649839867 19.062525620187472 38.24802814023791 10-11 25.544232205741118 26.06022472027089 27.251074279497463 21.14446879449053 12-13 25.991415666692962 23.351338689120084 27.26961427436193 23.38763136982502 14-15 23.2379156849046 24.76085130056193 24.53576921481537 27.465463799718098 16-17 23.673112384392855 27.711700802792137 25.281852395037756 23.333334417777245 18-19 23.72806889125293 26.960225516700664 26.54505942098294 22.766646171063464 20-21 24.611261074118307 26.084117589072008 26.233691997419417 23.07092933939027 22-23 24.24971655651546 25.79210457397265 26.10486026276878 23.85331860674311 24-25 23.878853576104635 26.103635437032104 26.26216964787219 23.75534133899107 26-27 23.67853949969354 26.265096968048628 26.193085032318347 23.86327849993949 28-29 23.780688361464435 26.3763178878788 26.244102098958184 23.598891651698587 30-31 24.032172009148315 26.234485765130344 26.017815140266578 23.715527085454767 32-33 23.66337848002103 26.322111943658598 25.99237694187581 24.022132634444564 34-35 23.959406304597493 26.34300485094172 26.067028236970618 23.630560607490168 36-37 24.13418668703801 26.006243170667865 26.048945560065835 23.81062458222829 38-39 23.833213232459443 26.21602541475468 26.036963958351294 23.913797394434585 40-41 24.05015933021125 26.18790066383521 26.093213460190217 23.668726545763313 42-43 23.989776743653408 26.12672491424229 25.89871545616857 23.984782885935736 44-45 23.917595970598015 26.20041733277897 25.99519943678944 23.886787259833575 46-47 24.041163721818183 26.307569834161004 25.927997002464704 23.723269441556116 48-49 23.877949656250287 26.322993947454375 25.831118881573893 23.96793751472144 50-51 23.8239325699758 26.49737581612265 25.93434188149315 23.744349732408402 52-53 24.14554940028395 26.243953714439645 25.787139516453315 23.823357368823096 54-55 23.901648874764607 26.357970781341216 25.768298017111263 23.972082326782914 56-57 23.834279655764846 26.379590406623137 25.84576110121936 23.940368836392658 58-59 23.90887336980481 26.140677081045734 25.86540041351689 24.085049135632566 60-61 23.771227927030637 26.18855326312406 26.10746498523575 23.932753824609552 62-63 23.706348630515606 26.23380160070069 26.16397958177717 23.895870187006533 64-65 23.702575790481443 26.275421767694883 25.98700676503914 24.034995676784536 66-67 23.753017611088183 26.323637559065148 25.817184779707276 24.106160050139394 68-69 23.937519062704606 26.106165539510123 26.020520620254654 23.93579477753061 70-71 23.65707051950568 26.064249012137395 26.101809832931615 24.176870635425303 72-73 23.724705142435656 25.9969210751802 26.019485107332073 24.25888867505207 74-75 23.632579429887627 26.260174768526053 26.00148676873426 24.10575903285206 76-77 23.823815079976622 25.94919423144836 26.06346630047552 24.163524388099503 78-79 23.723257079325368 26.05600021471602 26.10392092594656 24.116821780012053 80-81 23.791757944751808 26.122799748772778 26.07035500892 24.015087297555414 82-83 23.76685144829289 26.064782500276124 26.157094692138433 24.01127135929255 84-85 23.66767567486675 26.14007922739544 26.00537671401669 24.186868383721116 86-87 23.671472199211166 26.013111854550758 26.198346327620015 24.117069618618064 88-89 23.662239836139385 25.978631883590253 26.019899591129246 24.339228689141112 90-91 23.476479064930654 26.12503539969982 26.117813128949578 24.28067240641995 92-93 23.557624812209266 26.005399263234008 26.305701761877685 24.131274162679038 94-95 23.525875046402682 25.921359614033797 26.357325214395843 24.195440125167675 96-97 23.639660226977334 25.673968075598815 26.428336021191406 24.25803567623245 98-99 23.435239469986588 25.923617895581668 26.205401832059184 24.43574080237256 100-101 23.417956595904602 25.820987187340105 26.410120626584703 24.35093559017059 102-103 23.279012384239376 25.68976478274223 26.52673556343344 24.504487269584953 104-105 23.21057524704397 25.934114558127014 26.27352194541079 24.581788249418228 106-107 23.141714853558597 25.978003284441982 26.3194791748352 24.560802687164223 108-109 23.1029110299107 25.92422137160804 26.3542733764215 24.618594222059755 110-111 23.01222684347909 26.01604561703324 26.39105724971512 24.580670289772545 112-113 23.07975976470332 26.02280644420886 26.453747743178376 24.44368604790944 114-115 23.021543291079272 26.182300799149345 26.26273307709102 24.533422832680362 116-117 22.8993094868131 26.044049822101105 26.423174877084488 24.63346581400131 118-119 22.996060597532704 26.01594923625174 26.266446236680707 24.721543929534846 120-121 22.81875296983405 26.123933238862584 26.430770677324695 24.62654311397867 122-123 22.806620271409002 26.102635680100466 26.377102996821307 24.71364105166922 124-125 22.833116649338457 26.227253877037636 26.277741139338985 24.66188833428492 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 756.0 1 570.5 2 343.5 3 262.0 4 209.0 5 195.0 6 195.5 7 219.5 8 267.0 9 310.5 10 301.5 11 301.0 12 339.5 13 392.0 14 478.5 15 583.0 16 673.0 17 755.5 18 874.0 19 1018.5 20 1255.0 21 1659.0 22 2200.5 23 2830.0 24 3601.0 25 4844.5 26 6725.5 27 8964.5 28 11582.5 29 15274.0 30 19743.5 31 25280.5 32 31590.5 33 36438.5 34 41289.5 35 47260.0 36 53803.5 37 61152.5 38 69149.0 39 75470.0 40 81045.0 41 87558.0 42 93055.5 43 99500.0 44 105318.0 45 111349.0 46 118258.5 47 121658.0 48 124811.5 49 127817.5 50 130086.5 51 131991.0 52 131306.5 53 130036.5 54 127742.0 55 121343.5 56 111925.5 57 101708.5 58 92355.5 59 81879.0 60 69714.0 61 58983.5 62 48262.0 63 36282.5 64 26143.0 65 19330.5 66 14478.0 67 10912.0 68 7851.5 69 5712.5 70 4382.0 71 3226.0 72 2359.0 73 1841.5 74 1383.0 75 1018.0 76 750.0 77 503.0 78 328.0 79 205.0 80 139.5 81 103.0 82 76.0 83 53.0 84 45.5 85 42.5 86 27.5 87 17.5 88 18.5 89 16.5 90 14.0 91 11.5 92 8.0 93 7.5 94 8.0 95 8.0 96 7.0 97 7.0 98 6.5 99 6.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03689206176524937 2 0.017925507965301943 3 0.02296807372686601 4 0.020723318645911683 5 0.01343599780339329 6 0.004652173573572011 7 0.004619640891239339 8 0.005530555996554138 9 0.0027327453159443978 10-11 0.004684706255904681 12-13 0.006815596948694659 14-15 0.0020983580104573054 16-17 0.0019519609399602838 18-19 6.50653646653428E-5 20-21 0.003611127738926526 22-23 8.783824229821279E-4 24-25 0.0015453024108018916 26-27 0.0011711765639761704 28-29 0.0022447550809543268 30-31 0.0013663726579721989 32-33 0.0 34-35 0.006571601831199623 36-37 0.007596381324678772 38-39 8.783824229821279E-4 40-41 8.783824229821279E-4 42-43 0.0018218302106295984 44-45 8.458497406494565E-4 46-47 0.020837183034076032 48-49 0.0027327453159443978 50-51 0.009922468111464779 52-53 0.0014314380226375417 54-55 2.7652779982770693E-4 56-57 9.759804699801421E-5 58-59 0.006408938419536266 60-61 6.994526701524351E-4 62-63 0.009857402746799434 64-65 0.010068865181961798 66-67 4.879902349900711E-5 68-69 0.0030743384804374474 70-71 0.004896168691067046 72-73 0.011597901251597355 74-75 0.001691699481298913 76-77 8.946487641484635E-4 78-79 1.463970704970213E-4 80-81 0.003920188221086904 82-83 7.64518034817778E-4 84-85 6.994526701524351E-4 86-87 0.013989053403048703 88-89 0.0 90-91 2.114624351623641E-4 92-93 0.008604894476991585 94-95 0.006343873054870924 96-97 0.004326846750245296 98-99 7.482516936514422E-4 100-101 0.01641273823683272 102-103 0.008068105218502509 104-105 0.007791577418674801 106-107 0.018055638694632628 108-109 0.011923228074924068 110-111 0.019324413305606813 112-113 0.003106871162770119 114-115 0.014281847544042747 116-117 0.005725752090550167 118-119 0.0046359072324056744 120-121 0.00707585840735603 122-123 0.007872909124506479 124-125 0.05758284772882838 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3073832.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.21266251505847 #Duplication Level Percentage of deduplicated Percentage of total 1 71.40808540893154 35.855900934851505 2 13.299729877488268 13.356296957595164 3 5.67451684469158 8.547977977755382 4 3.0024182034198463 6.0303764790955565 5 1.811130117365305 4.547083267706114 6 1.1203517237874985 3.37535058028234 7 0.7790897700851411 2.738412018595378 8 0.5577005443986053 2.240290337628124 9 0.4139922735615835 1.87088888845706 >10 1.8254530628059047 15.451959397685764 >50 0.07601801629209329 2.587806225040159 >100 0.028966187211419508 2.4336427574185535 >500 0.002155974582841383 0.7414635694341606 >1k 3.919953784185089E-4 0.22255060845472746 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.50653646653428E-5 7 0.0 0.0 0.0 0.0 6.50653646653428E-5 8 0.0 0.0 0.0 0.0 6.50653646653428E-5 9 0.0 0.0 0.0 0.0 6.50653646653428E-5 10-11 0.0 0.0 0.0 0.0 6.50653646653428E-5 12-13 0.0 0.0 0.0 0.0 9.75980469980142E-5 14-15 0.0 0.0 0.0 0.0 1.138643881643499E-4 16-17 0.0 0.0 0.0 3.25326823326714E-5 1.62663411663357E-4 18-19 0.0 0.0 0.0 3.25326823326714E-5 1.62663411663357E-4 20-21 0.0 0.0 0.0 1.301307293306856E-4 2.439951174950355E-4 22-23 0.0 0.0 0.0 2.9279414099404264E-4 3.25326823326714E-4 24-25 0.0 0.0 0.0 6.343873054870924E-4 3.25326823326714E-4 26-27 0.0 0.0 0.0 0.0010898448581444919 3.25326823326714E-4 28-29 0.0 0.0 0.0 0.002228488739787991 3.25326823326714E-4 30-31 0.0 0.0 0.0 0.007596381324678773 3.415931644930497E-4 32-33 1.62663411663357E-5 0.0 0.0 0.020332926457919627 3.5785950565938543E-4 34-35 3.25326823326714E-5 0.0 0.0 0.036517935918423644 3.5785950565938543E-4 36-37 3.25326823326714E-5 0.0 0.0 0.06368272566620427 3.5785950565938543E-4 38-39 3.25326823326714E-5 0.0 0.0 0.10952127507293827 3.5785950565938543E-4 40-41 3.25326823326714E-5 0.0 0.0 0.16264714532219066 3.903921879920568E-4 42-43 3.25326823326714E-5 0.0 0.0 0.21491089948962727 3.903921879920568E-4 44-45 3.25326823326714E-5 0.0 0.0 0.27595847788688516 3.903921879920568E-4 46-47 3.25326823326714E-5 0.0 0.0 0.34930341020589284 3.903921879920568E-4 48-49 3.25326823326714E-5 0.0 0.0 0.42546241954667663 4.229248703247282E-4 50-51 6.50653646653428E-5 0.0 0.0 0.5051837576028879 4.229248703247282E-4 52-53 6.50653646653428E-5 0.0 0.0 0.5858810761290792 5.205229173227424E-4 54-55 6.50653646653428E-5 0.0 0.0 0.6764845964255691 5.205229173227424E-4 56-57 6.50653646653428E-5 0.0 0.0 0.7838424481233848 5.367892584890782E-4 58-59 6.50653646653428E-5 0.0 0.0 0.8981460274992257 5.530555996554139E-4 60-61 6.50653646653428E-5 0.0 0.0 1.0178337658011238 5.530555996554139E-4 62-63 6.50653646653428E-5 0.0 0.0 1.1427917986409146 5.855882819880852E-4 64-65 6.50653646653428E-5 0.0 0.0 1.2772005756983464 5.855882819880852E-4 66-67 6.50653646653428E-5 0.0 0.0 1.4339430391771573 5.855882819880852E-4 68-69 6.50653646653428E-5 0.0 0.0 1.6110997608197195 5.855882819880852E-4 70-71 6.50653646653428E-5 1.62663411663357E-5 0.0 1.7991223983613938 5.855882819880852E-4 72-73 6.50653646653428E-5 3.25326823326714E-5 0.0 1.9882674134435452 5.855882819880852E-4 74-75 6.50653646653428E-5 3.25326823326714E-5 0.0 2.1878554195544844 5.855882819880852E-4 76-77 6.50653646653428E-5 3.25326823326714E-5 0.0 2.4096307150163057 5.855882819880852E-4 78-79 6.50653646653428E-5 3.25326823326714E-5 0.0 2.6570092314739386 5.855882819880852E-4 80-81 6.50653646653428E-5 3.25326823326714E-5 0.0 2.9163109760064962 5.855882819880852E-4 82-83 6.50653646653428E-5 3.25326823326714E-5 0.0 3.1934894294808567 6.181209643207566E-4 84-85 6.50653646653428E-5 3.25326823326714E-5 0.0 3.4871131538743825 6.181209643207566E-4 86-87 6.50653646653428E-5 3.25326823326714E-5 1.62663411663357E-5 3.7968080233402475 6.343873054870924E-4 88-89 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 4.139214504891614 6.669199878197637E-4 90-91 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 4.50932256544925 6.831863289860994E-4 92-93 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 4.892150904798961 7.482516936514423E-4 94-95 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 5.287764588305412 7.482516936514423E-4 96-97 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 5.698294506661393 8.458497406494565E-4 98-99 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 6.13973047323341 8.783824229821279E-4 100-101 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 6.621962423450599 8.783824229821279E-4 102-103 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 7.121827087492095 8.946487641484635E-4 104-105 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 7.626994578753816 9.109151053147993E-4 106-107 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 8.142474930314993 9.109151053147993E-4 108-109 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 8.686437645258426 9.109151053147993E-4 110-111 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 9.265568189803476 9.109151053147993E-4 112-113 6.50653646653428E-5 3.25326823326714E-5 3.25326823326714E-5 9.870415819732504 9.109151053147993E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6655 0.0 35.594013 1 GGTATCA 4485 0.0 33.04295 1 TCAACGC 7345 0.0 30.702177 4 CAACGCA 7565 0.0 30.12197 5 ATCAACG 7875 0.0 28.713297 3 TATCAAC 8230 0.0 28.198233 2 AACGCAG 8095 0.0 27.929768 6 GTACATG 12690 0.0 26.358252 1 ACGCAGA 8945 0.0 24.943161 7 ACATGGG 12660 0.0 24.864058 3 TACATGG 13060 0.0 24.330732 2 CATGGGG 9220 0.0 24.135841 4 CGCAGAG 9425 0.0 23.609718 8 GTATATA 1390 0.0 21.837215 1 GCAGAGT 10475 0.0 20.220716 9 AGAGTAC 9510 0.0 18.300676 10-11 CAGAGTA 10040 0.0 17.275341 10-11 GTACCGT 595 1.8189894E-12 16.999357 6 ATGGGGA 5090 0.0 16.948986 5 GTATAGC 1095 0.0 16.849596 1 >>END_MODULE