FastQCFastQC Report
Mon 15 Aug 2016
SRR2920967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073832
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35350.1150030320459934No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79950.043.7512471
GGTATCA50250.042.5725061
TCAACGC99200.034.2310944
CAACGCA101700.033.230235
ATCAACG103250.033.003233
TATCAAC103700.032.854662
AACGCAG107650.031.4421566
GTACATG121900.027.9127881
ACGCAGA121350.027.7471877
CGCAGAG125500.026.7352818
ACATGGG121550.026.6130943
CATGGGG88200.026.4773834
TACATGG125700.026.2988912
GCAGAGT139600.023.5243249
AGAGTAC127800.021.97404510-11
GAGTACT69750.020.60222812-13
GTACTTT72050.020.02714314-15
GTATAGG7750.019.9913651
AGTACTT79150.019.73420512-13
CAGAGTA133050.019.63127510-11
Produced by FastQC (version 0.11.5)