FastQCFastQC Report
Mon 15 Aug 2016
SRR2920966_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920966_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3717132
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79550.042.1954351
GGTATCA51800.036.191561
TCAACGC93850.035.059554
ATCAACG97250.034.0182843
CAACGCA97950.033.833645
AACGCAG99700.033.1190766
TATCAAC101350.032.9360962
GTACATG156250.031.6905541
TACATGG159000.030.7615152
ACATGGG161000.029.8246143
ACGCAGA117850.027.9174677
CATGGGG104600.027.1332744
CGCAGAG121050.027.1303088
GCAGAGT136550.022.525759
AGAGTAC118700.020.7507110-11
ATGGGGA58550.019.1042045
ATGGGGG59250.018.9789165
CAGAGTA128350.018.17077810-11
GTATATG15850.018.0234741
GTATAGC19000.016.9147931
Produced by FastQC (version 0.11.5)