FastQCFastQC Report
Mon 15 Aug 2016
SRR2920966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3717132
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41470.11156450725989821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98750.046.1661071
GGTATCA61850.042.683791
TCAACGC124250.035.67314
CAACGCA127000.034.8555645
ATCAACG128100.034.832083
TATCAAC130600.034.1602482
AACGCAG133400.033.4464346
ACGCAGA155250.028.511557
GTACATG158900.028.277851
CGCAGAG157550.028.057948
TACATGG161200.027.3801352
ACATGGG162900.026.2207323
CATGGGG104150.023.6812924
GCAGAGT178500.023.531629
AGAGTAC159300.022.42804110-11
AGTACTT85350.020.7760212-13
TATACCG4301.8189894E-1220.755145
GAGTACT76400.020.4449312-13
CAGAGTA165300.019.97626310-11
GTATAAT18400.018.4610311
Produced by FastQC (version 0.11.5)