##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920966_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3717132 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.78489437555621 31.0 30.0 34.0 26.0 34.0 2 29.826637579725443 31.0 30.0 34.0 26.0 34.0 3 29.962824564745077 31.0 30.0 34.0 26.0 34.0 4 33.49957790038126 35.0 33.0 37.0 28.0 37.0 5 32.99023306140325 35.0 33.0 37.0 26.0 37.0 6 32.75578187699549 35.0 33.0 37.0 25.0 37.0 7 32.5812161096243 35.0 33.0 37.0 25.0 37.0 8 32.4944354948923 35.0 33.0 37.0 25.0 37.0 9 33.791175293209925 37.0 33.0 39.0 25.0 39.0 10-11 33.683299651451705 37.0 33.0 39.0 23.5 39.0 12-13 33.57338170933935 37.0 33.0 39.0 22.5 39.0 14-15 34.41785535192186 38.0 33.0 41.0 22.0 41.0 16-17 34.398646859998514 38.0 33.0 41.0 22.0 41.0 18-19 34.440861126266164 38.5 33.0 41.0 22.0 41.0 20-21 34.41982474660571 39.0 33.0 41.0 21.5 41.0 22-23 34.388918660946125 39.0 33.0 41.0 22.0 41.0 24-25 34.3584570039482 39.0 33.0 41.0 21.5 41.0 26-27 34.214850992647015 38.0 33.0 41.0 19.0 41.0 28-29 34.09319120224948 38.0 33.0 40.5 18.5 41.0 30-31 33.927052092850076 38.0 33.0 40.0 18.0 41.0 32-33 33.78939367770636 38.0 33.0 40.0 17.0 41.0 34-35 33.66260964098127 38.0 33.0 40.0 16.0 41.0 36-37 33.52075040649619 38.0 32.5 40.0 16.0 41.0 38-39 33.37488283978078 38.0 32.0 40.0 15.0 41.0 40-41 33.187915710284166 38.0 32.0 40.0 11.5 41.0 42-43 33.04121161153276 38.0 32.0 40.0 9.0 41.0 44-45 32.82224158302691 38.0 31.5 40.0 8.5 41.0 46-47 32.669389195756295 38.0 31.0 40.0 7.5 41.0 48-49 32.46122884524951 38.0 31.0 40.0 4.0 41.0 50-51 32.256299211327445 37.5 31.0 40.0 2.0 41.0 52-53 32.017136329837086 37.0 30.5 40.0 2.0 41.0 54-55 31.842066275827708 37.0 30.0 40.0 2.0 41.0 56-57 31.70251971143344 37.0 30.0 40.0 2.0 41.0 58-59 31.631929939534025 36.5 30.0 40.0 2.0 41.0 60-61 31.368758897989096 36.0 30.0 40.0 2.0 41.0 62-63 31.11067739859655 36.0 30.0 39.0 2.0 41.0 64-65 30.844496106137743 35.0 29.0 39.0 2.0 41.0 66-67 30.532256051170634 35.0 29.0 39.0 2.0 41.0 68-69 30.215008775582895 35.0 29.0 38.5 2.0 40.0 70-71 29.858029927374115 35.0 29.0 37.5 2.0 40.0 72-73 29.578647731638263 35.0 29.0 37.0 2.0 39.5 74-75 29.31356284899218 34.0 29.0 36.5 2.0 39.0 76-77 28.97866365789539 34.0 28.0 36.0 2.0 39.0 78-79 28.648478719615014 34.0 28.0 35.5 2.0 37.5 80-81 28.381401440680612 34.0 28.0 35.0 2.0 37.0 82-83 28.122472513755227 34.0 27.0 35.0 2.0 37.0 84-85 27.9195593538244 34.0 27.0 35.0 2.0 36.0 86-87 27.684482552677707 34.0 26.0 35.0 2.0 36.0 88-89 27.553650906128702 34.0 26.0 35.0 2.0 35.5 90-91 27.36424964192824 34.0 26.0 35.0 2.0 35.0 92-93 27.50064310333881 34.0 27.0 35.0 2.0 35.0 94-95 27.440531033065277 34.0 26.5 35.0 2.0 35.0 96-97 27.377564342616836 34.0 27.0 35.0 2.0 35.0 98-99 27.3273389268931 34.0 26.5 35.0 2.0 35.0 100-101 27.190574346028065 34.0 26.0 35.0 2.0 35.0 102-103 25.902281113503637 31.5 24.0 34.0 2.0 35.0 104-105 26.952273150375074 33.5 25.0 35.0 2.0 35.0 106-107 27.14387907128399 34.0 26.0 35.0 2.0 35.0 108-109 27.11787299993651 34.0 26.0 35.0 2.0 35.0 110-111 27.053114874586107 34.0 25.5 35.0 2.0 35.0 112-113 26.945487139009323 34.0 25.0 35.0 2.0 35.0 114-115 26.84671286895381 34.0 25.0 35.0 2.0 35.0 116-117 26.71335830957846 34.0 25.0 35.0 2.0 35.0 118-119 26.57642975821144 34.0 25.0 35.0 2.0 35.0 120-121 26.426630665792874 34.0 25.0 35.0 2.0 35.0 122-123 26.18311106519758 34.0 24.5 35.0 2.0 35.0 124-125 25.06266753507812 32.5 20.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 144232.0 3 44493.0 4 21793.0 5 16748.0 6 18102.0 7 21591.0 8 24109.0 9 25566.0 10 26962.0 11 27741.0 12 29041.0 13 30943.0 14 33358.0 15 28355.0 16 22623.0 17 20237.0 18 19043.0 19 18272.0 20 17780.0 21 18788.0 22 20767.0 23 26862.0 24 36159.0 25 46912.0 26 57476.0 27 67486.0 28 79430.0 29 93286.0 30 109880.0 31 130629.0 32 158469.0 33 195436.0 34 249644.0 35 339490.0 36 533275.0 37 731696.0 38 230106.0 39 352.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.448978586614835 19.814076574848873 13.990148287093373 24.746796551442916 2 15.946993471032869 20.75861276733348 38.53513748655884 24.759256275074808 3 18.546024600870105 24.981201353879165 29.829597503163154 26.643176542087577 4 13.172084428737662 17.095014653277595 35.906940912813 33.825960005171744 5 13.707992873792893 37.83078513936653 34.34113931594977 14.120082670890799 6 32.71710525253819 36.55474732191523 17.006625196192005 13.721522229354575 7 28.299643601713143 32.318816522007936 21.12290077588663 18.258639100392287 8 26.422428011552373 35.513735801011556 19.60212428544292 18.461711901993144 9 26.509554273824325 15.581511315585306 19.355373453201185 38.55356095738918 10-11 25.216502257791003 26.099033351694175 27.703368697902693 20.981095692612136 12-13 25.96700588578058 23.439162216375056 27.773855897330957 22.81997600051341 14-15 23.145198043202452 24.87695337650107 25.313003780036407 26.664844800260067 16-17 23.218408313959966 27.919219653879257 25.871357434716824 22.991014597443954 18-19 23.12618828200529 26.87051467279316 27.657122371245674 22.346174673955872 20-21 24.121924517523162 25.94433149506366 27.404224105025836 22.529519882387337 22-23 23.70984410505549 25.86034050748502 27.201930149668136 23.227885237791355 24-25 23.311029216182476 26.106715062739493 27.45540264127066 23.126853079807372 26-27 23.179085139675028 26.287629782808462 27.37400455560025 23.15928052191626 28-29 23.141193818599632 26.40595106436223 27.423923192629246 23.02893192440889 30-31 23.58380008083652 26.12468791558557 27.321937643932877 22.96957435964503 32-33 23.30943961526073 26.145309469753748 27.283225592739207 23.26202532224632 34-35 23.43207603758284 26.249739471521096 27.341745684107753 22.976438806788313 36-37 23.521408040061175 26.057336587442613 27.278852030333294 23.14240334216292 38-39 23.40607984516339 26.13979268105289 27.290787173125775 23.163340300657953 40-41 23.5258063283379 26.07391583064077 27.31486191107473 23.085415929946603 42-43 23.538824471632278 25.950461775117482 27.150406983221732 23.36030677002851 44-45 23.54028530270456 26.126855262793182 27.001913087331133 23.330946347171125 46-47 23.52031164337568 26.113239864363862 26.864263473653416 23.50218501860704 48-49 23.488492195275022 26.251484865007136 26.77280906048884 23.487213879229003 50-51 23.48938531340591 26.4031398580116 26.70238217930644 23.405092649276053 52-53 23.619713791774583 26.338196763310922 26.57544775203612 23.46664169287838 54-55 23.462830266697292 26.278798868477836 26.61488350200807 23.6434873628168 56-57 23.454955798601357 26.391687467641432 26.417843415213287 23.735513318543923 58-59 23.283830751952063 26.352115304582092 26.775433217666894 23.588620725798954 60-61 23.51163212885325 26.46836340597249 26.619073130484157 23.40093133469011 62-63 23.277481496594866 26.438127148816175 26.65047520582742 23.633916148761536 64-65 23.267652580005144 26.3561870882056 26.597522373609845 23.778637958179406 66-67 23.239162743078975 26.560450343716507 26.443254075695684 23.757132837508834 68-69 23.420480211583087 26.29995745410304 26.52576556376543 23.753796770548444 70-71 23.285453134976677 26.16425314493443 26.561621337581975 23.988672382506916 72-73 23.365515871563385 26.225032134990645 26.485516646678736 23.92393534676723 74-75 23.322592446235355 26.277971144242862 26.36361031044433 24.03582609907745 76-77 23.257776071789287 26.154660234498074 26.46742927581192 24.120134417900715 78-79 23.389098874956098 26.204978396627425 26.389162117466906 24.016760610949568 80-81 23.29161270791638 26.242822785448794 26.391644662708757 24.073919843926067 82-83 23.29499540921873 26.235167766377344 26.36696349188428 24.102873332519646 84-85 23.360158867797857 26.192857272594566 26.230408087619466 24.216575771988115 86-87 23.257413365520602 26.122241644026584 26.39711175790654 24.223233232546278 88-89 23.198382342809406 26.02846159477079 26.377624508039304 24.395531554380494 90-91 23.143887382045953 26.117814902512137 26.37934440400878 24.358953311433126 92-93 23.15024913430002 26.07308316209499 26.351657181730797 24.425010521874196 94-95 22.977809177846662 26.039297954616796 26.466374172423784 24.51651869511276 96-97 23.04240997162462 25.899698514069502 26.462228288983596 24.59566322532229 98-99 23.043909871392465 25.869699298621786 26.288779778435096 24.797611051550653 100-101 22.89616212912884 25.976891669306045 26.393573024117174 24.733373177447945 102-103 22.901415485435965 25.883490954849194 26.393075399714043 24.8220181600008 104-105 22.811237706630795 26.01084718743159 26.24330998389835 24.93460512203927 106-107 22.763229905648853 26.079565359412054 26.241705327156268 24.915499407782825 108-109 22.798377296028846 25.99395192565257 26.180342507156475 25.0273282711621 110-111 22.569106303625077 26.186771684478344 26.163441369465275 25.080680642431304 112-113 22.61648527086543 26.22837857163133 26.066146809318685 25.088989348184555 114-115 22.53499733754662 26.258019312271745 26.05651235558646 25.15047099459518 116-117 22.47645178033339 26.26017651444643 26.112175277806703 25.151196427413474 118-119 22.48459719991159 26.202039913360387 25.945379267593644 25.36798361913438 120-121 22.39376541699417 26.338726789844337 25.917647877030213 25.349859916131283 122-123 22.380420073339305 26.299364805204238 25.89500318808061 25.42521193337584 124-125 22.301041539482345 26.349517932434214 25.759548701673268 25.589891826410177 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 80.0 1 97.5 2 202.0 3 321.0 4 283.5 5 219.5 6 273.0 7 558.0 8 886.5 9 940.5 10 821.0 11 725.5 12 739.5 13 852.5 14 1000.0 15 1119.0 16 1300.0 17 1497.5 18 1751.0 19 2073.0 20 2389.5 21 2781.5 22 3330.5 23 4233.5 24 5300.0 25 6709.0 26 9121.0 27 12349.0 28 15803.0 29 20713.0 30 27087.5 31 34204.0 32 42286.0 33 49533.0 34 57390.0 35 66550.0 36 76030.5 37 84318.0 38 91068.0 39 98432.5 40 105873.0 41 112427.0 42 118388.0 43 126069.0 44 133168.5 45 138401.5 46 144967.5 47 150384.5 48 154064.0 49 157211.5 50 158800.0 51 157816.5 52 152541.0 53 147511.0 54 142780.5 55 135032.5 56 124167.5 57 111397.0 58 99182.5 59 87014.5 60 73707.5 61 60394.5 62 49066.0 63 38025.0 64 28468.0 65 21690.5 66 16215.5 67 12172.5 68 8918.5 69 6351.0 70 4788.0 71 3671.0 72 2957.0 73 2475.0 74 1877.0 75 1324.0 76 928.0 77 585.0 78 351.5 79 208.5 80 131.0 81 87.0 82 55.0 83 33.5 84 23.0 85 17.5 86 10.5 87 7.5 88 7.5 89 7.5 90 6.5 91 6.0 92 7.5 93 7.0 94 4.5 95 6.0 96 7.0 97 3.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04947362644103034 2 1.3451230679997376E-4 3 0.0029323682882394274 4 0.12515025024669557 5 0.01907384510423628 6 0.0029592707495994224 7 0.005999248883278829 8 0.013289815911837404 9 0.030964733025353956 10-11 0.023916288149035334 12-13 0.02158922524139579 14-15 0.0312741113309939 16-17 0.041698815107991866 18-19 0.045303744930231156 20-21 0.023284080307075457 22-23 0.025382472293155045 24-25 0.022934348309395523 26-27 0.022248335544715656 28-29 0.022113823237915685 30-31 0.0266334367463948 32-33 0.025638045676074993 34-35 0.03033252518339408 36-37 0.03268649055239362 38-39 0.03268649055239362 40-41 0.03599549329967297 42-43 0.033735686545433415 44-45 0.030910928102633968 46-47 0.04312464556007158 48-49 0.03513461453615314 50-51 0.024561947221675207 52-53 0.033251442240953506 54-55 0.03369533285339343 56-57 0.02582636290559496 58-59 0.030413232567474063 60-61 0.04604356261763101 62-63 0.05393943502678947 64-65 0.04738868568563075 66-67 0.042236864335191755 68-69 0.030803318257193985 70-71 0.03859158082091246 72-73 0.04186022987615183 74-75 0.025786009213554967 76-77 0.02183134739363574 78-79 0.034354443156713294 80-81 0.02540937475451504 82-83 0.041254924495551945 84-85 0.02245010400491562 86-87 0.03704468929271277 88-89 0.03734061636767271 90-91 0.040084667426392176 92-93 0.028624218887034415 94-95 0.025113447679555097 96-97 0.01900658895083629 98-99 0.027440510587194645 100-101 0.026445119516874838 102-103 0.03128756256167389 104-105 0.02834174304275447 106-107 0.0158724522023969 108-109 0.019975077559796103 110-111 0.02520760629431508 112-113 0.014406268058277188 114-115 0.017083062963596664 116-117 0.016383598968236802 118-119 0.009590727474838127 120-121 0.006765969032038679 122-123 0.0043581987403191495 124-125 0.07722351533386493 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3717132.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.91937758261992 #Duplication Level Percentage of deduplicated Percentage of total 1 73.85550523155939 41.299538835987306 2 13.779017116022382 15.410281216564767 3 5.166057767339978 8.66648204716532 4 2.4967160054071345 5.584592200917283 5 1.3594449709328025 3.8009658316192576 6 0.8282450647372597 2.7788969103590584 7 0.5523555608495408 2.1621165416903687 8 0.36871081311260917 1.6494463341791041 9 0.2942164785934431 1.4807162121745998 >10 1.2132875235963996 11.849173388357634 >50 0.06185587602671366 2.3549230241882557 >100 0.02376573805741262 2.314109055004084 >500 4.350990663598551E-4 0.15774253839736638 >1k 3.8675469853614386E-4 0.4910158633955681 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4147 0.11156450725989821 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.6902461359994747E-5 0.0 0.0 0.0 0.0 3 5.3804922719989495E-5 0.0 0.0 0.0 0.0 4 5.3804922719989495E-5 0.0 0.0 0.0 0.0 5 1.3451230679997373E-4 0.0 0.0 0.0 0.0 6 2.1521969087995798E-4 0.0 0.0 0.0 0.0 7 3.497319976799317E-4 0.0 0.0 5.3804922719989495E-5 2.6902461359994747E-5 8 3.497319976799317E-4 0.0 0.0 5.3804922719989495E-5 2.6902461359994747E-5 9 3.497319976799317E-4 0.0 0.0 8.070738407998425E-5 2.6902461359994747E-5 10-11 3.766344590399265E-4 0.0 0.0 8.070738407998425E-5 2.6902461359994747E-5 12-13 5.245979965198975E-4 0.0 0.0 1.614147681599685E-4 2.6902461359994747E-5 14-15 8.205250714798399E-4 0.0 0.0 1.8831722951996325E-4 2.6902461359994747E-5 16-17 9.281349169198189E-4 0.0 0.0 2.4212215223995274E-4 2.6902461359994747E-5 18-19 0.0012375132225597584 0.0 0.0 2.6902461359994747E-4 5.3804922719989495E-5 20-21 0.0014527329134397164 0.0 0.0 2.9592707495994225E-4 5.3804922719989495E-5 22-23 0.0014527329134397164 0.0 0.0 4.4389061243991335E-4 5.3804922719989495E-5 24-25 0.0019100747565596271 0.0 0.0 8.339763021598372E-4 5.3804922719989495E-5 26-27 0.0019100747565596271 0.0 0.0 0.0013854767600397296 6.725615339998687E-5 28-29 0.0022329042928795643 0.0 0.0 0.0036452835142792885 8.070738407998425E-5 30-31 0.0025288313678395063 0.0 0.0 0.013249462219797413 8.070738407998425E-5 32-33 0.002555733829199501 0.0 0.0 0.035457444072473074 8.070738407998425E-5 34-35 0.0028247584427994483 0.0 0.0 0.06437759003446744 8.070738407998425E-5 36-37 0.0031879416711593778 0.0 0.0 0.11058256742025842 8.070738407998425E-5 38-39 0.0034435150540793277 0.0 0.0 0.18526380015560384 8.070738407998425E-5 40-41 0.003456966284759325 0.0 0.0 0.26880939390906755 8.070738407998425E-5 42-43 0.0037528933597192676 0.0 0.0 0.3514672064376514 8.070738407998425E-5 44-45 0.004210235202839178 0.0 0.0 0.4498629588618322 9.415861475998161E-5 46-47 0.0050173090436390205 0.0 0.0 0.56318957734081 1.0760984543997899E-4 48-49 0.00532668734927896 0.0 0.0 0.681143419173707 1.0760984543997899E-4 50-51 0.00532668734927896 0.0 0.0 0.8036303257457631 1.0760984543997899E-4 52-53 0.005340138579958957 0.0 0.0 0.9249200727872995 1.0760984543997899E-4 54-55 0.00538049227199895 0.0 0.0 1.0623781991061927 1.0760984543997899E-4 56-57 0.0057033218083188866 0.0 0.0 1.2226361614276815 1.0760984543997899E-4 58-59 0.0057033218083188866 0.0 0.0 1.392323436455848 1.0760984543997899E-4 60-61 0.0057571267310388766 0.0 0.0 1.5655483851528544 1.0760984543997899E-4 62-63 0.005837834115118861 0.0 0.0 1.746642303797659 1.0760984543997899E-4 64-65 0.00589163903783885 0.0 0.0 1.9374076573013816 1.0760984543997899E-4 66-67 0.006295175958238771 0.0 0.0 2.162930990882218 1.0760984543997899E-4 68-69 0.006510395649118729 0.0 0.0 2.4091423172488895 1.0760984543997899E-4 70-71 0.006779420262718676 0.0 0.0 2.6678901906093193 1.0760984543997899E-4 72-73 0.007034993645638627 0.0 0.0 2.9322203247019476 1.0760984543997899E-4 74-75 0.007236762105838587 0.0 0.0 3.206719589188654 1.0760984543997899E-4 76-77 0.00727711579787858 0.0 0.0 3.5144299422242744 1.0760984543997899E-4 78-79 0.007371274412638561 0.0 0.0 3.8557280182678473 1.0760984543997899E-4 80-81 0.008326311790918375 0.0 0.0 4.218104172786976 1.0760984543997899E-4 82-83 0.008474275328398347 0.0 0.0 4.591631397539824 1.0760984543997899E-4 84-85 0.008743299941998293 0.0 0.0 4.974897313304989 1.0760984543997899E-4 86-87 0.00901232455559824 0.0 0.0 5.395315528208307 1.0760984543997899E-4 88-89 0.009039227016958236 0.0 0.0 5.855858764230057 1.0760984543997899E-4 90-91 0.009657983628238116 0.0 0.0 6.339295994869163 1.0760984543997899E-4 92-93 0.00968488608959811 0.0 0.0 6.838148335867546 1.0760984543997899E-4 94-95 0.00968488608959811 0.0 0.0 7.354204800905645 1.0760984543997899E-4 96-97 0.009711788550958105 0.0 0.0 7.9038758914130565 1.0760984543997899E-4 98-99 0.010074971779318034 0.0 0.0 8.483556677567545 1.0760984543997899E-4 100-101 0.010465057469037958 0.0 0.0 9.106254499436663 1.0760984543997899E-4 102-103 0.010828240697397885 0.0 0.0 9.742578417984618 1.0760984543997899E-4 104-105 0.012119558842677635 0.0 0.0 10.388116429548372 1.0760984543997899E-4 106-107 0.012415485917637577 0.0 0.0 11.055539593428481 1.0760984543997899E-4 108-109 0.012482742071037563 0.0 0.0 11.759698068295664 1.0760984543997899E-4 110-111 0.012724864223277517 0.0 0.0 12.502878563365519 1.0760984543997899E-4 112-113 0.012778669145997505 0.0 0.0 13.256725884364613 1.0760984543997899E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9875 0.0 46.166107 1 GGTATCA 6185 0.0 42.68379 1 TCAACGC 12425 0.0 35.6731 4 CAACGCA 12700 0.0 34.855564 5 ATCAACG 12810 0.0 34.83208 3 TATCAAC 13060 0.0 34.160248 2 AACGCAG 13340 0.0 33.446434 6 ACGCAGA 15525 0.0 28.51155 7 GTACATG 15890 0.0 28.27785 1 CGCAGAG 15755 0.0 28.05794 8 TACATGG 16120 0.0 27.380135 2 ACATGGG 16290 0.0 26.220732 3 CATGGGG 10415 0.0 23.681292 4 GCAGAGT 17850 0.0 23.53162 9 AGAGTAC 15930 0.0 22.428041 10-11 AGTACTT 8535 0.0 20.77602 12-13 TATACCG 430 1.8189894E-12 20.75514 5 GAGTACT 7640 0.0 20.44493 12-13 CAGAGTA 16530 0.0 19.976263 10-11 GTATAAT 1840 0.0 18.461031 1 >>END_MODULE