FastQCFastQC Report
Mon 15 Aug 2016
SRR2920965_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920965_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662334
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85800.034.1309431
GGTATCA60950.031.4450761
AACGCAG99350.028.7467566
TCAACGC100950.028.5322574
CAACGCA102650.027.9416775
ATCAACG102600.027.9009653
TATCAAC105500.027.5289022
GTACATG119900.026.3103831
ACGCAGA109300.025.8032047
CATGGGG67650.025.5989534
CGCAGAG111050.025.2884738
TACATGG121950.025.2307572
ACATGGG122000.025.122853
GCAGAGT121400.021.8092379
GTATAGG6300.021.72991
GTATAGC12200.020.0029721
AGAGTAC108250.019.78597510-11
GAGTACT42200.019.73685612-13
ATGGGGA38650.019.5532345
CAGAGTA118600.018.78667610-11
Produced by FastQC (version 0.11.5)