##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920965_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2662334
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.051447714674417	31.0	28.0	33.0	16.0	34.0
2	28.07559795277377	31.0	28.0	34.0	16.0	34.0
3	28.183746291787582	31.0	28.0	34.0	16.0	34.0
4	31.52390534020149	35.0	32.0	37.0	17.0	37.0
5	31.668411251180355	35.0	32.0	37.0	17.0	37.0
6	31.569976945041457	35.0	32.0	37.0	17.0	37.0
7	31.46334344225781	35.0	32.0	37.0	17.0	37.0
8	31.458605869887098	35.0	32.0	37.0	16.0	37.0
9	32.582554630636125	37.0	32.0	39.0	13.0	39.0
10-11	32.53551395129236	37.0	32.0	39.0	12.0	39.0
12-13	32.461026490290095	37.0	32.0	39.0	10.5	39.0
14-15	33.3082301093702	38.0	32.0	40.0	10.0	41.0
16-17	33.169329618297326	38.0	32.0	40.0	10.0	41.0
18-19	33.29539005999999	38.0	32.0	40.0	10.0	41.0
20-21	33.331771671022494	38.0	32.0	40.0	10.0	41.0
22-23	33.281407404179944	38.0	32.0	40.0	10.0	41.0
24-25	33.21626080724657	38.0	32.0	40.0	10.0	41.0
26-27	33.064397817854555	38.0	32.0	40.0	10.0	41.0
28-29	32.97930856909764	38.0	32.0	40.0	9.0	41.0
30-31	32.84093242996559	38.0	31.0	40.0	8.0	41.0
32-33	32.72832709945484	38.0	31.0	40.0	8.0	41.0
34-35	32.54374901871816	38.0	31.0	40.0	4.0	41.0
36-37	32.370768280764175	38.0	30.0	40.0	2.0	41.0
38-39	32.223315895000404	38.0	30.0	40.0	2.0	41.0
40-41	32.1391008791534	38.0	30.0	40.0	2.0	41.0
42-43	31.905081030404148	38.0	30.0	40.0	2.0	41.0
44-45	31.71713522796163	37.5	30.0	40.0	2.0	41.0
46-47	31.499605797018706	37.0	30.0	40.0	2.0	41.0
48-49	31.31472535001243	37.0	30.0	40.0	2.0	41.0
50-51	31.091880282488972	37.0	29.0	40.0	2.0	41.0
52-53	30.921629479997627	36.5	29.0	40.0	2.0	41.0
54-55	30.712629219324096	36.0	28.5	40.0	2.0	41.0
56-57	30.519991856769288	36.0	28.0	40.0	2.0	41.0
58-59	30.274549324014192	36.0	28.0	39.0	2.0	41.0
60-61	30.098186966774264	35.0	28.0	39.0	2.0	41.0
62-63	29.992917680501392	35.0	28.0	39.0	2.0	41.0
64-65	29.78481850887229	35.0	28.0	39.0	2.0	41.0
66-67	29.532638091238738	35.0	28.0	39.0	2.0	40.0
68-69	29.232313075669694	35.0	28.0	38.0	2.0	40.0
70-71	28.906564503176536	34.0	27.0	37.5	2.0	40.0
72-73	28.624395924778785	34.0	26.0	37.0	2.0	39.5
74-75	28.316450340190222	34.0	26.0	36.5	2.0	39.0
76-77	28.020742889509734	34.0	26.0	36.0	2.0	39.0
78-79	27.691183375188835	34.0	26.0	36.0	2.0	38.0
80-81	27.3643770841675	34.0	26.0	35.0	2.0	37.0
82-83	27.074581739180736	33.5	25.5	35.0	2.0	37.0
84-85	26.821484832481573	33.0	25.0	35.0	2.0	36.0
86-87	26.73334224781714	33.0	25.0	35.0	2.0	36.0
88-89	26.79963727315957	34.0	25.0	35.0	2.0	36.0
90-91	26.697259059156366	34.0	25.0	35.0	2.0	35.5
92-93	26.53865893610644	33.0	25.0	35.0	2.0	35.0
94-95	26.44950727444415	33.0	25.0	35.0	2.0	35.0
96-97	26.346539164507533	33.0	25.0	35.0	2.0	35.0
98-99	26.28082915216498	33.0	24.0	35.0	2.0	35.0
100-101	26.10741909166919	33.0	24.0	35.0	2.0	35.0
102-103	24.79162907433853	31.0	19.5	34.0	2.0	35.0
104-105	25.780028351063393	33.0	23.0	35.0	2.0	35.0
106-107	25.96042063092009	33.0	23.0	35.0	2.0	35.0
108-109	25.949050720157576	33.0	23.0	35.0	2.0	35.0
110-111	25.842279931819224	33.0	23.0	35.0	2.0	35.0
112-113	25.7444327045367	33.0	21.5	35.0	2.0	35.0
114-115	25.60542103282308	33.0	19.5	35.0	2.0	35.0
116-117	25.471786785579873	33.0	19.0	35.0	2.0	35.0
118-119	25.276392068012505	33.0	18.0	35.0	2.0	35.0
120-121	25.05389481560165	33.0	11.0	35.0	2.0	35.0
122-123	24.821775179222442	33.0	2.0	35.0	2.0	35.0
124-125	23.700876373888477	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	176490.0
3	24190.0
4	15253.0
5	14142.0
6	16892.0
7	17950.0
8	18188.0
9	19266.0
10	20319.0
11	21351.0
12	21891.0
13	22254.0
14	23473.0
15	21005.0
16	16765.0
17	15156.0
18	13578.0
19	13267.0
20	13413.0
21	14074.0
22	17082.0
23	21982.0
24	29866.0
25	38305.0
26	44721.0
27	50045.0
28	58236.0
29	66802.0
30	78425.0
31	92296.0
32	112305.0
33	138505.0
34	178094.0
35	240624.0
36	360944.0
37	467998.0
38	147025.0
39	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.5387934046891	19.697708654748656	13.388900453369384	24.374597487192858
2	15.337274194124022	20.767697714563717	39.79382798837653	24.101200102935735
3	18.518760639494932	25.27806863603694	30.002107704844676	26.201063019623444
4	12.371794775463343	16.98571765621689	37.362327312728326	33.28016025559144
5	13.243418487215648	38.3533576816185	34.727937591052495	13.675286240113364
6	31.457686862536438	37.376038187807055	17.572987265096568	13.59328768455994
7	27.19128455719425	33.45895544953698	21.529086471914198	17.82067352135457
8	25.489693025785655	36.08650510972595	20.179529693356344	18.24427217113206
9	25.96388716834187	16.73829108800883	19.84984977089075	37.44797197275855
10-11	24.301797846715843	27.149579532062145	28.113232884302057	20.435389736919948
12-13	24.94455074032204	24.86760074729957	27.820343083901033	22.367505428477358
14-15	22.489321135212535	26.180797725848787	25.467176858227646	25.862704280711036
16-17	22.39247603640056	28.93607215204185	26.313683037801994	22.357768773755602
18-19	22.239076689851835	28.065148850595005	27.997952172792743	21.697822286760417
20-21	23.463663647871506	27.21485157068751	27.504084951975987	21.817399829464993
22-23	22.966107122205134	27.01711236595076	27.237334281881427	22.779446229962687
24-25	22.634697340872673	27.209384960834647	27.505050140817982	22.650867557474697
26-27	22.52891916606427	27.305255641615506	27.479635494460318	22.686189697859906
28-29	22.51458638448857	27.42058106641655	27.63709022805206	22.42774232104282
30-31	22.93144629826688	27.315595083512157	27.29906806390097	22.453890554319994
32-33	22.49991924379135	27.389238164708107	27.260779451413686	22.850063140086856
34-35	22.710149584909995	27.477209538890516	27.299532499027567	22.51310837717192
36-37	22.941423622843494	27.11271620008125	27.20341566405475	22.74244451302051
38-39	22.658093600944902	27.41333922299643	27.202806304268336	22.725760871790335
40-41	22.82379733598568	27.37619905810694	27.29478443059342	22.505219175313957
42-43	22.802401349984645	27.28631112187894	27.173511903923465	22.737775624212954
44-45	22.891110321900783	27.379286033424915	27.075356586073934	22.65424705860037
46-47	22.80476041540457	27.380071513303783	27.011666229922692	22.803501841368952
48-49	22.71065566365786	27.409741002084147	27.02966654227274	22.84993679198525
50-51	22.61563477107453	27.656046531327828	26.865636038901826	22.86268265869582
52-53	22.790670245556623	27.49201999190167	26.763602448729994	22.953707313811705
54-55	22.81163591754652	27.3605162103582	26.786563100567527	23.04128477152775
56-57	22.795286600112043	27.481567329602385	26.63249727822435	23.090648792061224
58-59	22.777032236495504	27.166518445338262	26.884206117487082	23.17224320067916
60-61	22.84802191173256	27.219701027450515	26.955194683730493	22.977082377086433
62-63	22.586464154396573	27.285193844692845	27.022104951563243	23.10623704934734
64-65	22.678601883218796	27.17592686505602	26.952169728606428	23.193301523118752
66-67	22.82192665822794	27.315697152494245	26.727565703725688	23.13481048555213
68-69	23.031610895438572	27.055196518688383	26.83819676472411	23.07499582114893
70-71	22.780683399466565	26.94272900388085	27.085262753990953	23.191324842661633
72-73	22.981525238447638	26.931454051637672	26.98100307662255	23.106017633292137
74-75	22.92541502404607	27.057766719609898	26.830518364765915	23.18629989157812
76-77	22.87668827394384	26.937936124719347	27.03683566543559	23.148539935901226
78-79	22.9907937438024	27.00208840409868	26.91744418401995	23.08967366807897
80-81	22.948511714432478	26.951073498215294	26.869430726192455	23.23098406115977
82-83	22.961243052957535	26.984536585878892	26.918446717179684	23.135773643983885
84-85	23.014089733924852	26.916026898617098	26.8282266452265	23.24165672223155
86-87	22.86744948951848	26.85135600908733	26.961519714419612	23.319674786974574
88-89	22.96237061165128	26.763114620479623	26.88347893239541	23.391035835473687
90-91	22.79904894800202	26.82330978866137	27.020863421495957	23.356777841840653
92-93	22.873671379173686	26.818522462824912	27.00974619327477	23.29805996472663
94-95	22.73426901388796	26.85559833463962	27.025875742568957	23.384256908903463
96-97	22.854733457479128	26.748422587773046	26.986515813563443	23.41032814118438
98-99	22.74503676239281	26.83952350214542	26.815897309825587	23.599542425636184
100-101	22.67441303508688	26.92582438997818	26.92417143066446	23.475591144270478
102-103	22.56186478624362	26.94833319309296	26.995251110403228	23.494550910260187
104-105	22.485821906809146	27.077185736542432	26.824698759540105	23.612293597108316
106-107	22.39871306654835	27.144073544850606	26.856696225888076	23.60051716271297
108-109	22.457450593026433	27.184926651648013	26.83492967566396	23.522693079661597
110-111	22.428633417049085	27.28355220275116	26.757664499574535	23.53014988062522
112-113	22.415323114543042	27.331366953359982	26.733696371775405	23.51961356032157
114-115	22.33599944701714	27.377328529294754	26.69815889015739	23.588513133530707
116-117	22.481731041565403	27.25891247953734	26.689601625142835	23.569754853754425
118-119	22.49953985252743	27.12933712469809	26.685811305729494	23.68531171704499
120-121	22.468178793400174	27.23456533665803	26.695761486089648	23.60149438385215
122-123	22.508987784201523	27.170802740933752	26.621834648060705	23.698374826804024
124-125	22.54027270077722	27.245799569570156	26.505597786554073	23.708329943098548
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1392.0
1	972.5
2	492.0
3	394.0
4	330.5
5	290.0
6	301.0
7	354.5
8	426.0
9	504.5
10	564.0
11	595.0
12	638.0
13	721.5
14	805.5
15	960.5
16	1120.0
17	1293.0
18	1507.5
19	1731.5
20	2084.5
21	2624.0
22	3360.5
23	4335.0
24	5659.0
25	7340.5
26	10271.0
27	14284.0
28	17816.5
29	21991.5
30	27176.5
31	33149.5
32	39869.0
33	45797.5
34	52338.5
35	58947.5
36	65269.0
37	72242.0
38	76537.5
39	78769.5
40	82314.5
41	86842.0
42	90198.0
43	92872.5
44	96671.5
45	99989.0
46	102012.5
47	103404.5
48	105375.0
49	106948.0
50	106412.5
51	102552.5
52	96614.0
53	91877.5
54	87302.5
55	81468.5
56	74221.5
57	66759.5
58	60262.0
59	53406.0
60	45883.5
61	38756.0
62	31800.5
63	24260.0
64	17981.5
65	13916.5
66	10525.0
67	8011.0
68	6305.0
69	4864.0
70	3865.5
71	3036.5
72	2389.0
73	2133.5
74	1683.5
75	1289.0
76	1001.5
77	699.0
78	519.0
79	354.0
80	226.5
81	166.0
82	129.5
83	87.5
84	63.0
85	49.5
86	36.0
87	30.5
88	29.0
89	22.5
90	12.5
91	15.0
92	17.5
93	11.5
94	11.5
95	10.5
96	10.0
97	12.0
98	13.0
99	14.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034856633314978513
2	0.017991732066675332
3	0.02520344930425709
4	0.022010761985536
5	0.015324899129861243
6	0.004620006355325815
7	0.004394640191651386
8	0.005859520255535181
9	0.002516588827697802
10-11	0.004563664814407208
12-13	0.008094401378639945
14-15	0.0024790278004187304
16-17	0.002516588827697802
18-19	0.0
20-21	0.0046575673826048875
22-23	7.324400319418976E-4
24-25	0.0012019528729302936
26-27	0.0015963436593605463
28-29	0.003418053482395522
30-31	0.0010141477365349352
32-33	0.0
34-35	0.007530985969453869
36-37	0.009221232197012095
38-39	0.0015963436593605463
40-41	0.00135219698204658
42-43	0.0030612237232443412
44-45	0.0014460995502442594
46-47	0.022217347635570893
48-49	0.003474395023314129
50-51	0.011249527670081966
52-53	0.0021034175276280135
54-55	3.1926873187210924E-4
56-57	3.5682975915118085E-4
58-59	0.007944157269523659
60-61	6.009764364651468E-4
62-63	0.011493674347395932
64-65	0.011118064074605215
66-67	1.502441091162867E-4
68-69	0.004469762246209529
70-71	0.005915861796453788
72-73	0.012169772838419222
74-75	0.001821709823034976
76-77	0.0013709774956861159
78-79	5.258543819070034E-4
80-81	0.004751469950802566
82-83	0.0018780513639535836
84-85	0.0015400021184419387
86-87	0.014517337043361202
88-89	0.0
90-91	7.136595183023617E-4
92-93	0.01010391633807028
94-95	0.007793913160407372
96-97	0.005314885359988641
98-99	0.0012958554411279727
100-101	0.016583193543710142
102-103	0.008939524492419058
104-105	0.008131962405919017
106-107	0.018874416207733516
108-109	0.01168147948379129
110-111	0.020621003976210348
112-113	0.0030612237232443412
114-115	0.01528733810258217
116-117	0.006310252582884041
118-119	0.00540878792818632
120-121	0.008770499869663235
122-123	0.00933391527884931
124-125	0.0565481265686424
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2662334.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.10289948689109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.16341871613018	38.32086770713096
2	13.325078635053767	14.152006228243602
3	5.734496878436576	9.135552340305246
4	2.9807851322502414	6.331533330796156
5	1.6449372100652317	4.3675467664170515
6	1.079628245908208	3.439883413540326
7	0.7023359686240499	2.6107253443509846
8	0.4916437495747969	2.0886166893622953
9	0.347480032533441	1.6606977517192467
>10	1.4440459120423488	12.674504760541605
>50	0.05835912745969765	2.1243608578237945
>100	0.026719654964930634	2.447101566948897
>500	6.424421738275407E-4	0.22254705350460488
>1k	4.2829478253329724E-4	0.42405618931527855
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.756102727907167E-5
2	0.0	0.0	0.0	0.0	3.756102727907167E-5
3	0.0	0.0	0.0	0.0	3.756102727907167E-5
4	0.0	0.0	0.0	3.756102727907167E-5	3.756102727907167E-5
5	0.0	0.0	0.0	3.756102727907167E-5	3.756102727907167E-5
6	0.0	0.0	0.0	3.756102727907167E-5	7.512205455814334E-5
7	0.0	0.0	0.0	3.756102727907167E-5	7.512205455814334E-5
8	0.0	0.0	0.0	3.756102727907167E-5	7.512205455814334E-5
9	0.0	0.0	0.0	3.756102727907167E-5	7.512205455814334E-5
10-11	0.0	0.0	0.0	7.512205455814334E-5	7.512205455814334E-5
12-13	0.0	0.0	0.0	1.1268308183721502E-4	3.192687318721092E-4
14-15	0.0	0.0	0.0	1.3146359547675085E-4	4.3195181370932423E-4
16-17	0.0	0.0	0.0	1.6902462275582254E-4	4.5073232734886006E-4
18-19	0.0	0.0	0.0	1.8780513639535837E-4	4.695128409883959E-4
20-21	0.0	0.0	0.0	3.192687318721092E-4	5.258543819070034E-4
22-23	0.0	0.0	0.0	7.324400319418976E-4	5.446348955465393E-4
24-25	0.0	0.0	0.0	0.0017653682821163687	6.009764364651467E-4
26-27	0.0	0.0	0.0	0.0029109796141280546	6.197569501046826E-4
28-29	0.0	0.0	0.0	0.00685488747843058	6.385374637442184E-4
30-31	0.0	0.0	0.0	0.01825465925762883	6.573179773837542E-4
32-33	0.0	0.0	0.0	0.042744449043583564	7.136595183023618E-4
34-35	0.0	0.0	0.0	0.07852132752689933	7.887815728605051E-4
36-37	0.0	0.0	0.0	0.13373603762713468	7.887815728605051E-4
38-39	0.0	0.0	0.0	0.21683981048208076	8.639036274186485E-4
40-41	0.0	0.0	0.0	0.3103104268660506	9.014646546977201E-4
42-43	0.0	0.0	0.0	0.3941090787256595	9.390256819767918E-4
44-45	0.0	0.0	0.0	0.4945848266971762	9.765867092558634E-4
46-47	0.0	0.0	0.0	0.6130898677626473	0.0010141477365349352
48-49	0.0	0.0	0.0	0.7374544290836537	0.0010517087638140068
50-51	0.0	0.0	0.0	0.8630772848185089	0.0010892697910930785
52-53	0.0	0.0	0.0	0.9901274595899687	0.0012019528729302934
54-55	0.0	0.0	0.0	1.1314320442138364	0.0012019528729302934
56-57	0.0	0.0	0.0	1.296287392941682	0.0012207333865698294
58-59	0.0	0.0	0.0	1.4727303185851213	0.0012395139002093652
60-61	0.0	0.0	0.0	1.6523659315472816	0.0013146359547675085
62-63	0.0	0.0	0.0	1.8312503239638602	0.00135219698204658
64-65	0.0	0.0	0.0	2.029196937724568	0.00135219698204658
66-67	0.0	0.0	0.0	2.261192622713754	0.0014273190366047236
68-69	0.0	0.0	0.0	2.5224671284669764	0.0014273190366047236
70-71	0.0	0.0	0.0	2.7999492174911187	0.0014836605775233312
72-73	0.0	0.0	0.0	3.076905452133354	0.0016151241730000818
74-75	0.0	0.0	0.0	3.364266091331891	0.0016902462275582252
76-77	0.0	0.0	0.0	3.681468966703652	0.0016902462275582252
78-79	7.512205455814334E-5	0.0	0.0	4.036495796545437	0.0016902462275582252
80-81	7.512205455814334E-5	0.0	0.0	4.416857539286957	0.001727807254837297
82-83	7.512205455814334E-5	0.0	0.0	4.814365890981372	0.001727807254837297
84-85	7.512205455814334E-5	0.0	0.0	5.220607181518171	0.0017653682821163685
86-87	7.512205455814334E-5	0.0	0.0	5.659395102192287	0.0018592708503140476
88-89	7.512205455814334E-5	0.0	0.0	6.139594055441579	0.0019719539321512627
90-91	7.512205455814334E-5	0.0	0.0	6.659701600174884	0.0020095149594303342
92-93	7.512205455814334E-5	0.0	0.0	7.1868706180366555	0.002065856500348942
94-95	7.512205455814334E-5	0.0	0.0	7.730622829442137	0.002065856500348942
96-97	7.512205455814334E-5	0.0	0.0	8.293437262191746	0.0022536616367443
98-99	1.1268308183721502E-4	0.0	0.0	8.9103771352505	0.0023287836913024437
100-101	1.1268308183721502E-4	0.0	0.0	9.57103804406209	0.0023287836913024437
102-103	1.1268308183721502E-4	1.8780513639535835E-5	0.0	10.231060415409939	0.0023287836913024437
104-105	1.1268308183721502E-4	3.756102727907167E-5	0.0	10.900285238441157	0.0023287836913024437
106-107	1.1268308183721502E-4	3.756102727907167E-5	0.0	11.58879764898018	0.0023663447185815153
108-109	1.1268308183721502E-4	3.756102727907167E-5	0.0	12.312917913379763	0.002403905745860587
110-111	1.1268308183721502E-4	3.756102727907167E-5	0.0	13.07488091276301	0.002403905745860587
112-113	1.1268308183721502E-4	3.756102727907167E-5	0.0	13.855868572463109	0.002441466773139659
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8580	0.0	34.130943	1
GGTATCA	6095	0.0	31.445076	1
AACGCAG	9935	0.0	28.746756	6
TCAACGC	10095	0.0	28.532257	4
CAACGCA	10265	0.0	27.941677	5
ATCAACG	10260	0.0	27.900965	3
TATCAAC	10550	0.0	27.528902	2
GTACATG	11990	0.0	26.310383	1
ACGCAGA	10930	0.0	25.803204	7
CATGGGG	6765	0.0	25.598953	4
CGCAGAG	11105	0.0	25.288473	8
TACATGG	12195	0.0	25.230757	2
ACATGGG	12200	0.0	25.12285	3
GCAGAGT	12140	0.0	21.809237	9
GTATAGG	630	0.0	21.7299	1
GTATAGC	1220	0.0	20.002972	1
AGAGTAC	10825	0.0	19.785975	10-11
GAGTACT	4220	0.0	19.736856	12-13
ATGGGGA	3865	0.0	19.553234	5
CAGAGTA	11860	0.0	18.786676	10-11
>>END_MODULE