FastQCFastQC Report
Mon 15 Aug 2016
SRR2920965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662334
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52720.19802173581526583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44740.16804803604656668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33360.1253035870029831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108750.038.6769561
GGTATCA72100.037.844871
TCAACGC135850.030.3890934
ATCAACG137000.030.2627473
TATCAAC138150.030.0914082
CAACGCA137650.029.8270825
AACGCAG140650.029.3111926
CATGGGG63450.027.6032284
ACGCAGA155000.026.2924737
CGCAGAG155450.025.9881958
GTACATG119250.024.8298931
TACATGG120000.023.7238182
GCAGAGT166200.023.2704039
ACATGGG119700.023.2403353
GAGTACT93150.023.22072412-13
AGTACTT98700.022.63846612-13
AGAGTAC155050.022.0663210-11
GTACTTT101350.021.25391814-15
CAGAGTA164500.019.623110-11
TAAGGTG13200.018.932735
Produced by FastQC (version 0.11.5)