FastQCFastQC Report
Mon 15 Aug 2016
SRR2920964_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920964_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908505
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31300.042.0194661
GGTATCA21000.038.5409281
TCAACGC36300.035.733274
CAACGCA37700.034.2484785
AACGCAG40700.032.015576
ATCAACG40200.031.9705893
TATCAAC40300.031.8912542
GTACATG70500.031.6551761
TACATGG71900.030.2883132
ACATGGG71150.029.3531763
CATGGGG55600.027.5030754
ACGCAGA47700.027.3172687
CGCAGAG51050.025.4081048
GTCTTAG7100.023.4694141
ATGGGGA27950.021.7140715
GCAGAGT56750.021.5979659
GAGTACA50150.021.0041391
CGTATAC2055.938969E-520.317353
AGAGTAC50050.019.49574710-11
ATGGGGG30600.019.444715
Produced by FastQC (version 0.11.5)