##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920964_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1908505
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.076527962986734	31.0	27.0	31.0	10.0	34.0
2	27.100752159412735	31.0	27.0	31.0	10.0	34.0
3	27.219589678832385	31.0	27.0	33.0	10.0	34.0
4	30.511194888145432	35.0	30.0	37.0	10.0	37.0
5	30.622172852573087	35.0	31.0	37.0	10.0	37.0
6	30.49467095973026	35.0	30.0	37.0	10.0	37.0
7	30.346718504798257	35.0	30.0	37.0	8.0	37.0
8	30.343014558515698	35.0	30.0	37.0	8.0	37.0
9	31.379099347394952	37.0	30.0	39.0	8.0	39.0
10-11	31.318588895496738	37.0	30.0	39.0	7.5	39.0
12-13	31.19499398744043	37.0	30.0	39.0	7.0	39.0
14-15	31.945353038110984	38.0	30.0	40.0	2.0	41.0
16-17	31.821560855224376	37.0	30.0	40.0	2.0	41.0
18-19	31.869098587637968	38.0	30.0	40.0	2.0	41.0
20-21	31.890195205147485	38.0	30.0	40.0	2.0	41.0
22-23	31.807312267979388	37.5	30.0	40.0	2.0	41.0
24-25	31.72289357376585	37.5	30.0	40.0	2.0	41.0
26-27	31.566475330166806	37.0	30.0	40.0	2.0	41.0
28-29	31.474499149858136	37.0	30.0	40.0	2.0	41.0
30-31	31.318927380331726	37.0	30.0	40.0	2.0	41.0
32-33	31.225474651625227	37.0	30.0	40.0	2.0	41.0
34-35	31.046421151634394	37.0	29.0	40.0	2.0	41.0
36-37	30.87376087565922	37.0	29.0	40.0	2.0	41.0
38-39	30.712399757925706	37.0	28.5	40.0	2.0	41.0
40-41	30.605786990340604	37.0	28.0	40.0	2.0	41.0
42-43	30.36839122768869	36.0	28.0	40.0	2.0	41.0
44-45	30.160949539037098	36.0	27.5	40.0	2.0	41.0
46-47	29.936414366218585	36.0	27.0	40.0	2.0	41.0
48-49	29.747471974136825	35.5	27.0	40.0	2.0	41.0
50-51	29.48727249863113	35.0	26.0	39.5	2.0	41.0
52-53	29.279147028695235	35.0	26.0	39.0	2.0	41.0
54-55	29.052851839528845	35.0	26.0	39.0	2.0	40.5
56-57	28.83136643603239	35.0	26.0	39.0	2.0	40.0
58-59	28.564627810773352	34.0	25.0	39.0	2.0	40.0
60-61	28.358712185716044	34.0	24.5	38.0	2.0	40.0
62-63	28.23354300879484	34.0	25.0	38.0	2.0	40.0
64-65	28.00181424727732	34.0	24.0	38.0	2.0	40.0
66-67	27.735500037987848	34.0	23.5	37.5	2.0	40.0
68-69	27.43163654273895	34.0	23.0	37.0	2.0	40.0
70-71	27.110520014356787	33.5	22.5	36.5	2.0	39.0
72-73	26.842942774580102	33.0	22.0	36.0	2.0	39.0
74-75	26.55784291893393	33.0	22.0	35.5	2.0	38.5
76-77	26.298886039072467	33.0	21.5	35.0	2.0	37.5
78-79	25.995330376394087	33.0	20.0	35.0	2.0	37.0
80-81	25.704659144199255	32.0	20.0	35.0	2.0	36.5
82-83	25.44779316795083	32.0	19.5	35.0	2.0	36.0
84-85	25.21346708549362	32.0	18.0	35.0	2.0	36.0
86-87	25.155147353556842	32.0	18.5	35.0	2.0	35.0
88-89	25.25161736542477	32.5	18.5	35.0	2.0	35.0
90-91	25.159145247196104	33.0	18.0	35.0	2.0	35.0
92-93	25.009860859678124	32.0	18.0	35.0	2.0	35.0
94-95	24.916355733938346	32.0	17.0	35.0	2.0	35.0
96-97	24.817893848850275	32.0	15.5	35.0	2.0	35.0
98-99	24.741070890566178	32.0	14.0	35.0	2.0	35.0
100-101	24.567756699615668	32.0	9.0	35.0	2.0	35.0
102-103	23.288986929560046	30.0	7.0	34.0	2.0	34.5
104-105	24.261957657957407	32.0	7.0	34.5	2.0	35.0
106-107	24.437415411539398	32.0	7.0	35.0	2.0	35.0
108-109	24.422087969379174	32.0	4.5	35.0	2.0	35.0
110-111	24.304831268453583	32.0	2.0	35.0	2.0	35.0
112-113	24.201782547072185	32.0	2.0	35.0	2.0	35.0
114-115	24.06276535822542	32.0	2.0	35.0	2.0	35.0
116-117	23.91657370559679	32.0	2.0	35.0	2.0	35.0
118-119	23.69288369692508	32.0	2.0	35.0	2.0	35.0
120-121	23.45482170599501	32.0	2.0	35.0	2.0	35.0
122-123	23.200737750228583	32.0	2.0	35.0	2.0	35.0
124-125	22.061698816613003	30.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	164486.0
3	23126.0
4	14513.0
5	12614.0
6	13459.0
7	14708.0
8	15948.0
9	16720.0
10	17442.0
11	17603.0
12	17700.0
13	17644.0
14	17296.0
15	15458.0
16	13584.0
17	12055.0
18	11341.0
19	10826.0
20	11139.0
21	11923.0
22	14347.0
23	18860.0
24	24916.0
25	31113.0
26	35693.0
27	40807.0
28	46549.0
29	53781.0
30	61749.0
31	71805.0
32	85849.0
33	104113.0
34	130961.0
35	174125.0
36	249147.0
37	257195.0
38	57867.0
39	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.887513411602406	18.98275714765683	12.301826703489716	25.82790273725105
2	16.369100804158883	20.032229241617124	37.52070663267311	26.07796332155088
3	19.57309864292093	23.51107002561555	28.985764714267148	27.930066617196374
4	13.26614299990934	15.33577785685126	34.969403278777875	36.42867586446153
5	14.194977092620434	37.40214647622511	33.74954214061772	14.653334290536735
6	34.167866179909694	35.01825862640843	16.372085078020323	14.441790115661545
7	29.100274311405016	32.17167125598992	20.202471684617866	18.525582747987205
8	27.521684429392835	34.56660223213513	19.221629461794375	18.69008387667765
9	26.67078867460852	15.766837572801034	18.550555292108015	39.01181846048243
10-11	25.88800901272655	25.897912532012864	26.873409181715168	21.34066927354542
12-13	25.842071369023262	23.27835239535972	27.051213596175682	23.828362639441337
14-15	23.173791251803806	24.801646561472012	24.06906713363955	27.95549505308463
16-17	23.67513671288081	27.799486552189308	24.982125738909772	23.543250996020113
18-19	23.765545221402412	26.933916058358783	26.336458729858904	22.9640799903799
20-21	24.407565803607266	26.055550956204982	26.179157047328715	23.357726192859037
22-23	24.103078538936483	25.70272307401703	26.15719314755436	24.03700523949213
24-25	23.791213239272118	26.008377899928924	26.273092081184256	23.927316779614703
26-27	23.49629038896488	26.156117786405314	26.32971065846087	24.017881166168934
28-29	23.687881797558642	26.429527869829972	26.192691231830846	23.68989910078054
30-31	23.92293127745479	26.248029516706634	26.002022029590588	23.827017176247992
32-33	23.58131720412501	26.231952819602995	26.064072680991845	24.122657295280153
34-35	23.851587143560778	26.32203451997946	26.189047713863534	23.637330622596227
36-37	23.924879785334884	26.01871350789982	26.18950557543487	23.86690113133043
38-39	23.81731854713526	26.232498284639988	26.09105149276274	23.85913167546201
40-41	23.858795876027212	26.22384758287859	26.197125018895733	23.72023152219846
42-43	23.775206059016675	26.180819488869368	25.997114479502038	24.04685997261192
44-45	23.681200442023226	26.2028706358983	26.119323216122424	23.996605705956046
46-47	23.738879478503055	26.264749306011336	26.08632315046617	23.910048065019435
48-49	23.663430770028825	26.494207191351055	25.849246025778534	23.993116012841586
50-51	23.685138540362697	26.584532965003227	25.899415908234662	23.830912586399418
52-53	23.8774418416996	26.344617599808856	25.820697742413145	23.957242816078402
54-55	23.635599115012948	26.322627295484285	25.959646371959167	24.082127217543597
56-57	23.60363834615009	26.58217468179928	25.79236779373588	24.02181917831475
58-59	23.650228207380657	26.28098601936691	25.950065013100865	24.118720760151565
60-61	23.582962009716592	26.43495793528699	26.125210113975044	23.856869941021376
62-63	23.562663385807173	26.379043020933818	26.116682552216652	23.941611041042357
64-65	23.3867479409181	26.32294882378786	26.08759897832544	24.2027042569686
66-67	23.416580888334295	26.483013270338112	25.978639295846566	24.12176654548103
68-69	23.621904285336274	26.11555762817104	26.117994115751497	24.14454397074119
70-71	23.36967485455619	26.087717432140632	26.27496176254369	24.267645950759483
72-73	23.508562604358502	26.208855600715893	26.106491586736535	24.17609020818907
74-75	23.31078832934499	26.397487213639764	26.05291962866765	24.2388048283476
76-77	23.380914517610105	26.07408727045037	26.175764379477517	24.369233832462008
78-79	23.364968083615295	26.19344224070967	26.245760746973083	24.195828928701953
80-81	23.55821200211058	26.19499486764605	26.08359737403726	24.163195756206115
82-83	23.52610149900328	26.157380541749642	26.27176347516625	24.044754484080837
84-85	23.29861147498035	26.266780193869533	26.110689022792773	24.323919308357347
86-87	23.216893549926983	26.233954465449276	26.320728863019593	24.228423121604152
88-89	23.205519503485714	26.054608188084387	26.20409692403216	24.535775384397734
90-91	23.076131075404852	26.114085662360242	26.319691674816593	24.490091587418316
92-93	23.33216659182404	26.00006602219918	26.426249797610517	24.24151758836626
94-95	23.232162333305475	25.87642918916398	26.504341046236814	24.387067431293733
96-97	23.232987996705223	25.63003150157508	26.531536157646208	24.60544434407349
98-99	23.038918896543848	25.835599735812703	26.43059752071594	24.694883846927514
100-101	22.952502099329394	25.8289436279294	26.64551398526736	24.573040287473848
102-103	22.791528646349832	25.77779792279805	26.713903828080767	24.71676960277135
104-105	22.763127661118606	25.906204960268752	26.45033010905297	24.88033726955967
106-107	22.718027174612224	25.933369089631885	26.512946024670796	24.83565771108509
108-109	22.604667492879805	25.977870413796538	26.569547320789916	24.84791477253374
110-111	22.635100171482993	26.116401450989795	26.495760086252407	24.752738291274802
112-113	22.5495145676853	26.217248678052673	26.62252446810991	24.61071228615211
114-115	22.50750640599045	26.36925753391638	26.457474179535417	24.665761880557753
116-117	22.426339013193505	26.218757843452163	26.547351002573304	24.807552140781024
118-119	22.472415550254592	26.092800075453603	26.48219831984731	24.95258605444449
120-121	22.28004170967759	26.311863678521085	26.549676959595057	24.858417652206263
122-123	22.38500093010582	26.205063443697156	26.523893239571677	24.88604238662534
124-125	22.31616314211275	26.283465305864596	26.418937357021022	24.981434195001636
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	826.0
1	698.0
2	488.0
3	361.5
4	295.5
5	266.0
6	259.5
7	292.5
8	342.5
9	367.5
10	377.0
11	411.0
12	456.0
13	514.0
14	599.0
15	683.5
16	769.0
17	817.5
18	898.0
19	1075.0
20	1293.0
21	1578.0
22	2024.0
23	2627.5
24	3319.0
25	4220.0
26	5643.5
27	7610.0
28	9426.5
29	11240.5
30	13648.0
31	16868.5
32	20841.5
33	23289.5
34	25227.5
35	28268.5
36	32383.5
37	36417.0
38	39803.5
39	43314.0
40	46083.0
41	49363.0
42	52597.5
43	56810.0
44	62044.0
45	67284.0
46	72155.5
47	74706.0
48	77135.5
49	79635.5
50	80898.0
51	81891.0
52	81672.0
53	80197.5
54	78559.5
55	75117.0
56	69613.5
57	63551.0
58	58416.0
59	52455.0
60	44789.5
61	37827.5
62	31049.5
63	23454.0
64	17193.5
65	13097.0
66	9895.0
67	7715.5
68	5862.0
69	4407.5
70	3479.0
71	2619.0
72	1899.5
73	1474.5
74	1189.0
75	897.0
76	663.0
77	451.5
78	274.5
79	173.0
80	123.0
81	84.5
82	59.0
83	45.5
84	33.0
85	25.5
86	22.5
87	16.0
88	9.5
89	11.5
90	11.0
91	6.0
92	2.5
93	3.5
94	4.5
95	4.0
96	2.5
97	1.5
98	3.0
99	4.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033638895365744395
2	0.015719109983992705
3	0.015142742617912973
4	0.015404727784312854
5	0.008802701591035916
6	0.004820527061757764
7	0.004715732995197812
8	0.005658879594237375
9	0.002619851663998784
10-11	0.004846725578397751
12-13	0.004506144862077909
14-15	0.0022530724310389546
16-17	0.0018076976481591611
18-19	2.6198516639987846E-5
20-21	0.001205131765439441
22-23	9.693451156795503E-4
24-25	0.001650506548319234
26-27	8.645510491195988E-4
28-29	8.645510491195988E-4
30-31	0.0013885213819193557
32-33	2.6198516639987846E-5
34-35	0.002357866497598906
36-37	0.00230546946431893
38-39	7.073599492796719E-4
40-41	2.881836830398663E-4
42-43	5.501688494397447E-4
44-45	4.1917626623980554E-4
46-47	0.007990547575196292
48-49	9.431465990395624E-4
50-51	0.0037725863961582495
52-53	3.6677923295982983E-4
54-55	1.309925831999392E-4
56-57	2.6198516639987846E-5
58-59	0.0022792709476789426
60-61	8.907495657595867E-4
62-63	0.0032486160633584927
64-65	0.00364159381295831
66-67	2.6198516639987846E-5
68-69	0.0010479406655995137
70-71	0.0018862931980791248
72-73	0.006261445476957095
74-75	0.001362322865279368
76-77	6.54962915999696E-4
78-79	1.309925831999392E-4
80-81	0.0012575287987194165
82-83	1.8338961647991492E-4
84-85	2.619851663998784E-4
86-87	0.005658879594237375
88-89	0.0
90-91	2.6198516639987846E-5
92-93	0.0029342338636786387
94-95	0.0019124917147191126
96-97	0.001519513965119295
98-99	5.501688494397447E-4
100-101	0.00825253274159617
102-103	0.0063138425102370705
104-105	0.0063138425102370705
106-107	0.011343957705114737
108-109	0.010479406655995137
110-111	0.007990547575196292
112-113	0.002698447213918748
114-115	0.007073599492796718
116-117	0.003458204196478395
118-119	0.0024888590807988454
120-121	0.003929777495998176
122-123	0.006287643993597083
124-125	0.05766293512461325
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1908505.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.1291233384505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.26447394836129	49.06496927685224
2	10.595261915263073	12.953581448426075
3	3.6632823625410613	6.717997180900287
4	1.7078312841320842	4.175929168358978
5	0.9606806666914683	2.9362783481523813
6	0.6102567295664842	2.238267533387343
7	0.425892383887635	1.8224099644501766
8	0.30919074984363304	1.5120447585839552
9	0.22351440029861538	1.2296915409396474
>10	1.1646208818139614	12.487776050348133
>50	0.052652067548233254	2.1699214040047026
>100	0.02138677894255514	2.241919644444392
>500	8.689373725770439E-4	0.3848885341876848
>1k	8.68937372577044E-5	0.06432514696401964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.2397033279975685E-5
2	0.0	0.0	0.0	0.0	5.2397033279975685E-5
3	0.0	0.0	0.0	0.0	5.2397033279975685E-5
4	0.0	0.0	0.0	0.0	5.2397033279975685E-5
5	0.0	0.0	0.0	0.0	5.2397033279975685E-5
6	0.0	0.0	0.0	0.0	5.2397033279975685E-5
7	0.0	0.0	0.0	0.0	5.2397033279975685E-5
8	0.0	0.0	0.0	0.0	5.2397033279975685E-5
9	0.0	0.0	0.0	0.0	5.2397033279975685E-5
10-11	0.0	0.0	0.0	0.0	5.2397033279975685E-5
12-13	0.0	0.0	0.0	0.0	1.0479406655995137E-4
14-15	0.0	0.0	0.0	0.0	1.833896164799149E-4
16-17	0.0	0.0	0.0	0.0	2.881836830398663E-4
18-19	0.0	0.0	0.0	5.2397033279975685E-5	4.191762662398055E-4
20-21	0.0	2.6198516639987843E-5	0.0	7.859554991996353E-5	4.715732995197812E-4
22-23	0.0	5.2397033279975685E-5	0.0	1.8338961647991492E-4	5.501688494397447E-4
24-25	0.0	5.2397033279975685E-5	0.0	3.9297774959981763E-4	6.287643993597083E-4
26-27	0.0	5.2397033279975685E-5	0.0	7.859554991996353E-4	6.81161432639684E-4
28-29	0.0	7.859554991996353E-5	0.0	0.004741931511837799	6.81161432639684E-4
30-31	0.0	1.0479406655995137E-4	0.0	0.02053963704575047	6.81161432639684E-4
32-33	0.0	1.5719109983992706E-4	0.0	0.048493454300617495	7.335584659196597E-4
34-35	0.0	2.0958813311990274E-4	0.0	0.08336367994844132	7.335584659196597E-4
36-37	0.0	2.0958813311990274E-4	0.0	0.13607509542809687	7.335584659196597E-4
38-39	0.0	2.0958813311990274E-4	0.0	0.2217442448408571	7.859554991996354E-4
40-41	0.0	2.3578664975989057E-4	0.0	0.31810238904273236	7.859554991996354E-4
42-43	0.0	2.619851663998784E-4	0.0	0.41312440889596835	8.121540158396232E-4
44-45	0.0	2.619851663998784E-4	0.0	0.5204859300866385	8.38352532479611E-4
46-47	0.0	2.619851663998784E-4	0.0	0.6467627802913799	8.38352532479611E-4
48-49	0.0	2.619851663998784E-4	0.0	0.7792748774564384	8.38352532479611E-4
50-51	0.0	2.619851663998784E-4	0.0	0.9193321474138134	8.38352532479611E-4
52-53	0.0	2.619851663998784E-4	0.0	1.0613805046358276	8.645510491195989E-4
54-55	0.0	2.619851663998784E-4	0.0	1.217707053426635	8.907495657595867E-4
56-57	0.0	3.143821996798541E-4	0.0	1.3952544006958325	8.907495657595867E-4
58-59	0.0	3.143821996798541E-4	0.0	1.5860058003515842	8.907495657595867E-4
60-61	0.0	3.143821996798541E-4	0.0	1.7872104081466906	8.907495657595867E-4
62-63	0.0	3.143821996798541E-4	0.0	1.991768426071716	8.907495657595867E-4
64-65	0.0	3.143821996798541E-4	0.0	2.2128315094799333	8.907495657595867E-4
66-67	0.0	3.143821996798541E-4	0.0	2.4622937849258975	8.907495657595867E-4
68-69	0.0	3.143821996798541E-4	0.0	2.7345749683652913	8.907495657595867E-4
70-71	0.0	3.143821996798541E-4	0.0	3.0268718185176358	8.907495657595867E-4
72-73	0.0	3.143821996798541E-4	0.0	3.3136669801755825	9.693451156795503E-4
74-75	0.0	3.143821996798541E-4	0.0	3.610207990023605	9.95543632319538E-4
76-77	0.0	3.40580716319842E-4	0.0	3.9479330680296885	9.95543632319538E-4
78-79	0.0	3.6677923295982983E-4	0.0	4.318406291835756	9.95543632319538E-4
80-81	0.0	3.6677923295982983E-4	0.0	4.7059609484910965	0.0010217421489595259
82-83	0.0	3.6677923295982983E-4	0.0	5.111802169761148	0.0011003376988794895
84-85	0.0	3.6677923295982983E-4	0.0	5.529406525002555	0.0011003376988794895
86-87	0.0	4.1917626623980554E-4	0.0	5.979287452744426	0.0011003376988794895
88-89	0.0	4.715732995197812E-4	0.0	6.4671824281309185	0.0011003376988794895
90-91	0.0	4.715732995197812E-4	0.0	6.984707925837239	0.0011003376988794895
92-93	0.0	4.715732995197812E-4	0.0	7.513917961964994	0.0011003376988794895
94-95	0.0	4.715732995197812E-4	0.0	8.046638599322506	0.001152734732159465
96-97	0.0	4.97771816159769E-4	0.0	8.615041616343682	0.001152734732159465
98-99	0.0	5.239703327997568E-4	0.0	9.219729578911242	0.001152734732159465
100-101	0.0	5.763673660797325E-4	0.0	9.874587700844378	0.001152734732159465
102-103	0.0	5.763673660797325E-4	0.0	10.542361691481029	0.001152734732159465
104-105	0.0	5.763673660797325E-4	0.0	11.200363635410962	0.001178933248799453
106-107	0.0	5.763673660797325E-4	0.0	11.87696652615529	0.001205131765439441
108-109	0.0	6.287643993597083E-4	0.0	12.597923505571114	0.0012575287987194165
110-111	0.0	6.81161432639684E-4	0.0	13.351707226336845	0.001309925831999392
112-113	0.0	6.81161432639684E-4	0.0	14.129488788344805	0.001309925831999392
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3130	0.0	42.019466	1
GGTATCA	2100	0.0	38.540928	1
TCAACGC	3630	0.0	35.73327	4
CAACGCA	3770	0.0	34.248478	5
AACGCAG	4070	0.0	32.01557	6
ATCAACG	4020	0.0	31.970589	3
TATCAAC	4030	0.0	31.891254	2
GTACATG	7050	0.0	31.655176	1
TACATGG	7190	0.0	30.288313	2
ACATGGG	7115	0.0	29.353176	3
CATGGGG	5560	0.0	27.503075	4
ACGCAGA	4770	0.0	27.317268	7
CGCAGAG	5105	0.0	25.408104	8
GTCTTAG	710	0.0	23.469414	1
ATGGGGA	2795	0.0	21.714071	5
GCAGAGT	5675	0.0	21.597965	9
GAGTACA	5015	0.0	21.004139	1
CGTATAC	205	5.938969E-5	20.31735	3
AGAGTAC	5005	0.0	19.495747	10-11
ATGGGGG	3060	0.0	19.44471	5
>>END_MODULE