FastQCFastQC Report
Mon 15 Aug 2016
SRR2920964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908505
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33150.1736961653231194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24720.1295254662680999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.10767590339035003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.054.3159641
GTATCAA50250.047.6819531
TCAACGC63800.037.256294
TATCAAC64700.036.7351532
CAACGCA65100.036.4587335
ATCAACG66450.035.7705273
AACGCAG68200.034.7142946
ACGCAGA79450.029.8744567
GTACATG73200.028.824131
CGCAGAG83050.028.5809758
ACATGGG72950.027.6835543
TACATGG75950.027.3722762
CATGGGG52600.026.3886764
GCAGAGT89800.025.3746689
CGTACAC2651.3606041E-924.72833
AGAGTAC81400.022.87753710-11
ACCGTAT2954.780304E-922.1827018
AGTACTT55050.021.94014212-13
GAGTACT52650.021.64069212-13
ATGGGGG26950.021.631025
Produced by FastQC (version 0.11.5)