FastQCFastQC Report
Mon 15 Aug 2016
SRR2920963_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920963_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3801545
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71550.043.0875781
GGTATCA45150.041.127171
TCAACGC86900.034.7825664
CAACGCA87250.034.709865
AACGCAG92050.032.7025456
ATCAACG93100.032.531843
TATCAAC95400.031.374122
ACGCAGA105100.028.6419537
GTACATG146600.027.84981
CGCAGAG111600.026.8671258
TACATGG149200.026.7612042
ACATGGG149000.026.077593
CATGGGG104700.025.2889634
GCAGAGT128300.022.3035139
AGAGTAC110800.020.80646110-11
CAGAGTA120150.018.84070610-11
GTATAGA14100.016.8839041
ATGGGGA56050.016.3472865
GTATTAA26450.015.9758751
GTATTAG12300.015.9676431
Produced by FastQC (version 0.11.5)