FastQCFastQC Report
Mon 15 Aug 2016
SRR2920963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3801545
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94650.045.0718961
GGTATCA60500.043.824611
TCAACGC117500.035.999764
CAACGCA119350.035.2455375
ATCAACG124150.034.070563
TATCAAC125100.034.0427632
AACGCAG127050.033.5247466
ACGCAGA144200.028.9626987
CGCAGAG149250.027.7036918
GTACATG144300.025.1457391
CATGGGG98400.024.6421384
TACATGG146650.024.206962
ACATGGG144800.024.0688293
GCAGAGT165900.023.883659
AGAGTAC148000.022.67381110-11
CAGAGTA159900.019.57241610-11
AGTACTT84550.019.5312-13
GAGTACT79800.019.27571712-13
GTACTTT82650.018.89902314-15
TATACAG24000.017.8495985
Produced by FastQC (version 0.11.5)